Background Several variants of the SARS-CoV-2 have been documented globally during current COVID-19 pandemic. The N501Y, 69-70del, K417N, and E484K mutations among most relevant due to their potential pathogenic biological effects. This study aimed design, validate, propose a fast real-time RT-qPCR assay detect with possible clinical epidemiological relevance in Mexican population. Methods Targeting spike ( S ) gene (N501Y, E484K), specific primers, probes for three quantitative reverse...

The immunomodulatory effects of vitamin D are known to be beneficial in viral infections; it is also that its deficiency associated with a prognosis more critical Coronavirus Disease 2019. This study aimed determine baseline serum concentrations and the supplementation asymptomatic or mildly symptomatic 2019 outpatients.

Worldwide breast cancer ranks first in mortality and incidence rates women over 20 years old. Rather than one disease, is a heterogeneous group of diseases that express distinct molecular profiles. Neoadjuvant chemotherapy an important therapeutic strategy for patients independently their subtype, with the drawback resistance development. In addition, has adverse effects combined could contribute to lower overall survival. Although great efforts have been made find diagnostic prognostic...

SARS-CoV-2 has become a global pandemic due to its capacity for rapid transmission. In this context, an early and diagnosis of infected patients that do not require expensive equipment or highly trained personnel is crucial in order reduce the contagious rate. The aim study was evaluate chromatographic immunoassay's performance SARS-CoV-antigen.

Background:Currently, one of the most used strategies for treatment newly diagnosed patients with breast cancer is neoadjuvant chemotherapy based on application taxanes and anthracyclines. However, despite high number who develop a complete pathological clinical response, resistance relapse following this therapy continue to be challenge. As component innate immune system, cytotoxic function Natural Killer (NK) cells plays an important role in elimination tumor cells. NK systemic remains...

We aim to identify Th1 and Th2 cell clusters in young subjects, including their clinical metabolic characteristics the Th1/Th2 balance.A total of 100 participants were included. The frequencies cells peripheral blood determined by flow cytometry. Serum C-reactive protein was measured using a turbidimetric assay, insulin levels quantified with an enzyme-linked immunosorbent assay. Circulating cytokine analyzed multiplex system.A cluster analysis performed determine balance group people, 3...

Cervical cancer (CC) is the second most common in less developed countries and leading cause of death by women worldwide. The 99% CC patients are infected with Human Papilloma Virus (HPV), being HPV16 HPV18 infection frequent. Even though HPV considered to be a necessary factor for development CC, it not enough, as requires participation other factors such hormonal ones. Several studies have demonstrated requirement estrogen its receptors (ERα, ERβ, GPER) precursor lesions progress towards...

Abstract Background T‐cell activation pathways have been proposed as trigger mechanisms in the pathogenesis of rheumatoid arthritis (RA). CD28 and CTLA‐4 play major roles regulating stimulatory inhibitory co‐signals T cells. Objective To analyze association between soluble surface expression with clinical parameters RA patients. Methods A total 35 patients classified early (n = 14), chronic untreated 7), well 7 age‐ sex‐matched control subjects (CS) were included. Surface on cells was...

Macrophage migration inhibitory factor (MIF) significantly contributes to rheumatoid arthritis (RA) pathogenesis. We aimed evaluate the canonical (CD74/CD44) and non-canonical MIF receptors (CXCR2,4 7) expression sCD74 establish their association with RA clinical activity according DAS28-ESR.101 patients different activities (remission (n = 27), low 16), moderate 35) high 23)) 9 control subjects (CS) were included. Expression was evaluated by flow cytometry levels of soluble CD74 (sCD74)...

Natural killer (NK) cells play a crucial role in cervical cancer (CC). As estrogens and prolactin (PRL) have been reported to be involved CC, the present study attempted elucidate effects of both hormones on NK CC. For this purpose, NKL cells, as well CC-derived cell lines (HeLa, SiHa C33A) non-tumorigenic keratinocytes (HaCaT cells) were stimulated with 17β-estradiol (E2; 10 nM), PRL (200 ng/ml), or (E2 PRL) for 48 h. The expression hormone receptors (estrogen receptor α β, G...

Breast cancer ranks first in terms of mortality and incidence rates worldwide among women. The HER2+ molecular subtype is one the most aggressive subtypes; its treatment includes neoadjuvant chemotherapy use a HER2 antibody. Some patients develop resistance despite positive results obtained using this therapeutic strategy.To identify prognostic markers for survival patients.Patients treated with were assigned to sensitive resistant groups based on their response. Differentially expressed...

NKG2D, an activating immunoreceptor, is primarily restricted to NK cells and CD8+ T cells. The existence of atypical cytotoxic CD4+NKG2D+ cell population has also been found in patients with autoimmune dysfunctions. Nonetheless, contradictory evidence categorized this a regulatory rather than role other situations. These confounding data have led the proposal that two distinct subsets might exist. immune response elicited cervical cancer characterized by apparent contradictions concerning...

Abstract Background The NKG2D receptor confers important activating signals to NK cells via ligands expressed during cellular stress and viral infection. This has generated great interest because not only is it on cells, but also seen in virtually all CD8 + cytotoxic T classically considered absent CD4 cells. However, recent studies have identified a distinctive population of that do express NKG2D, which could represent particular effector involved infections chronic diseases. On the other...

Introduction Respiratory viral infections represent a significant global health burden. Historically, influenza, rhinovirus, respiratory syncytial virus, and adenovirus have been the prevalent viruses; however, landscape shifted with widespread emergence of SARS-CoV-2. The aim this study is to present comprehensive epidemiological analysis in Jalisco, Mexico. Methods Data encompassing individuals flu-like symptoms from July 2021 February 2023 was scrutinized for diagnosis through PCR...

Cervical cancer (CC) poses a significant health burden, particularly in low- and middle-income countries. NK cells play crucial role against CC; however, they can become exhausted lose their cytotoxic capacity. This work explores the expression of costimulatory receptors (ICOS, 4-1BB, OX-40) from CC patients. Peripheral blood tumor biopsies were collected, flow cytometry was used to evaluate cells. There is an increase peripheral (PD-1+TIGIT+) patients; this subpopulation has selectively...

Abstract Background Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed determine of MetS RA from western Mexico and analyze interrelation components with clinical variables RA. Methods case‐control included 216 260 control subjects (CS). was determined according NCEP/ATP III Latin American Consensus Diabetes Association (ALAD) criteria. Results observed 30.6% 33.3% controls ( p > 0.05) 28.7% 31.1% for...

Understanding the humoral immune response dynamics carried out by B cells in COVID-19 vaccination is little explored; therefore, we analyze changes induced different cellular subpopulations of after with BNT162b2 (Pfizer-BioNTech).This prospective cohort study evaluated thirty-nine immunized health workers (22 prior and 17 without COVID-19) ten subjects not vaccinated against SARS-CoV-2 (control group). cell (transitional, mature, naïve, memory, plasmablasts, early plasmablast,...

Due to the COVID-19 pandemic, rapid development of vaccines against SARS-CoV-2 has been promoted. BNT162b2 is a lipid-nanoparticle mRNA vaccine with 95% efficacy and most administered globally. Nevertheless, little known about cellular immune response triggered by vaccination behavior over time. Therefore, we evaluated T-cell spike protein neutralization antibodies (nAbs) in naïve previously infected subjects vaccinated BTN162b2. Methods: Forty-six BTN162b2 were included (twenty-six twenty...

Developing countries have reported lower molecular diagnostic testing levels due to a lack of resources. Therefore, antibody tests represent an alternative detect exposure SARS-CoV-2 and analyze possible risk factors. We aimed describe compare the clinical-epidemiological characteristics quality food intake in Mexican individuals with positive or negative test antibodies against SARS-CoV-2. carried out applied survey 1799 individuals; 42% were positive, diabetes was more prevalent these...

Abstract Few studies analyze the role of B-cell subpopulations in rheumatoid arthritis (RA) pathophysiology. Therefore, this study aimed to differences and activation according disease activity, RA subtype, absence disease-modifying antirheumatic drugs (DMARDs) therapy. These subgroups were compared with control subjects (CS). One hundred thirty-nine included, which 114 patients, 25 controls. Patients divided into 99 seropositive RA, 6 seronegative 9 without DMARDs. The patients...