A5081895979- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Liver Disease Diagnosis and Treatment
- Immune Cell Function and Interaction
- SARS-CoV-2 detection and testing
- Liver Disease and Transplantation
- HIV-related health complications and treatments
- Diet and metabolism studies
- Animal Virus Infections Studies
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Intensive Care Unit Cognitive Disorders
- Vitamin C and Antioxidants Research
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Dialysis and Renal Disease Management
- Long-Term Effects of COVID-19
- Lipid metabolism and disorders
- COVID-19 diagnosis using AI
- Cell death mechanisms and regulation
- Biosensors and Analytical Detection
- Diet, Metabolism, and Disease
- Pregnancy and preeclampsia studies
- Influenza Virus Research Studies
Universidad de Guadalajara
2016-2024
Background Several variants of the SARS-CoV-2 have been documented globally during current COVID-19 pandemic. The N501Y, 69-70del, K417N, and E484K mutations among most relevant due to their potential pathogenic biological effects. This study aimed design, validate, propose a fast real-time RT-qPCR assay detect with possible clinical epidemiological relevance in Mexican population. Methods Targeting spike ( S ) gene (N501Y, E484K), specific primers, probes for three quantitative reverse...
Gut microbiota undergoes profound alterations in alcohol cirrhosis. Microbiota-derived products, e.g., short chain fatty acids (SCFA), regulate the homeostasis of gut-liver axis. The objective was to evaluate composition and functions intestinal patients with alcohol-decompensated Fecal samples 18 healthy controls (HC) were obtained. Microbial characterized by 16S rRNA amplicon sequencing, SCFA quantification performed gas chromatography (GC), metagenomic predictive profiles analyzed...
The immunomodulatory effects of vitamin D are known to be beneficial in viral infections; it is also that its deficiency associated with a prognosis more critical Coronavirus Disease 2019. This study aimed determine baseline serum concentrations and the supplementation asymptomatic or mildly symptomatic 2019 outpatients.
Introduction The variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been classified into interest (VOIs) or concern (VOCs) to prioritize global monitoring and research on with potential risks public health. SARS-CoV-2 high-rate mutation can directly impact the clinical disease progression, epidemiological behavior, immune evasion, vaccine efficacy, transmission rates. Therefore, surveillance is crucial for controlling COVID-19 pandemic. In present study, we aimed...
Abstract Background The study of stool microbiota has taken great relevance in the last years, given its role maintenance intestinal metabolic, physiological, and immunological homeostasis, as well as, effect over HIV biomarkers levels such CD4/CD8 ratio, high sensitivity C-Reactive Protein (hs-CRP), related to poor outcomes (rapid progression AIDS). Several efforts have been made characterize gut microbiome. In infection, most studies report presence a dysbiotic pattern; however, few them...
SARS-CoV-2 has become a global pandemic due to its capacity for rapid transmission. In this context, an early and diagnosis of infected patients that do not require expensive equipment or highly trained personnel is crucial in order reduce the contagious rate. The aim study was evaluate chromatographic immunoassay's performance SARS-CoV-antigen.
Understanding the evolution of SARS-CoV-2 virus in various regions world during Covid-19 pandemic is essential to help mitigate effects this devastating disease. We describe phylogenomic and population genetic patterns Mexico pre-vaccination stage, including asymptomatic carriers. A real-time quantitative PCR screening reconstructions directed at sequence/structure analysis spike glycoprotein revealed mutation concern E484K genomes from central Mexico, addition nationwide prevalence imported...
Animal digestive systems host microorganism ecosystems, including integrated bacteria, viruses, fungi, and others, that produce a variety of compounds from different substrates with healthy properties. Among these substrates, α-galacto-oligosaccharides (GOS) are considered prebiotics promote the grow gut microbiota metabolic output Short Chain Fatty Acids (SCFAs). In this regard, we evaluated
Gut microbiota disturbances may influence cognitive function, increasing uremic toxins and inflammation in dialysis patients; therefore, we aimed to evaluate the association of gut profile with impairment (CI) patients on automated peritoneal (APD). In a cross-sectional study, function was evaluated using Montreal Cognitive Assessment 39 APD classified as normal CI. The analyzed 16S rRNA gene sequencing approach. All had clinical, biochemical urea clearance evaluations. Eighty-two percent...
A remarkable increase in metabolic syndrome (MetS) has occurred HIV-infected subjects. Gut dysbiosis is involved the pathogenesis of disorders. Therefore, aim to explore profile gut microbiota Mexican population with HIV infection and MetS.In all, 30 patients MetS were compared a group without MetS, treated integrase inhibitors undetectable viral load included study. Stool samples analysed by 16S rRNA next-generation sequencing. High-sensitivity C-reactive protein >3 mg L-1 higher scores...
Aim Intestinal dysfunction in cirrhosis patients is linked to death by bacterial infections. Currently, there no effective therapy for this complication. This study aims evaluate butyrate, a novel postbiotic, on the intestinal inflammatory response, tight junction proteins and microbiota cholestasis model. Methods Results Wistar rats underwent 15 days of bile duct ligation (BDL). We administered butyrate at concentration 1%. The BDL group did not receive treatment. results showed that could...
Introduction Respiratory viral infections represent a significant global health burden. Historically, influenza, rhinovirus, respiratory syncytial virus, and adenovirus have been the prevalent viruses; however, landscape shifted with widespread emergence of SARS-CoV-2. The aim this study is to present comprehensive epidemiological analysis in Jalisco, Mexico. Methods Data encompassing individuals flu-like symptoms from July 2021 February 2023 was scrutinized for diagnosis through PCR...
Cervical cancer (CC) is the fourth leading among women and one of principal gynecological malignancies. In tumor microenvironment, cancer-associated fibroblasts (CAFs) play a crucial role during malignant progression, exhibiting variety heterogeneous phenotypes. CAFs express phenotypic markers like fibroblast activation protein (FAP), vimentin, S100A4, α-smooth muscle actin (αSMA), functional such as MMP9. This study aimed to evaluate expression αSMA, FAP, MMP9 in mesenchymal stem cells...
Microbial community control is crucial for maintaining homeostasis of the gut–liver axis in metabolic dysfunction-associated steatotic liver disease (MASLD). Here, we show that supplementation with a mixture Mexican foodstuffs (MexMix)—Opuntia ficus indica (nopal), Theobroma cacao (cocoa) and Acheta domesticus (crickets)—enriches several beneficial taxa MASLD mice overweight/obese humans. Thus, MexMix induces an important prebiotic effect. In mice, restoration intestinal health was observed...
HIV/HCV coinfection is associated with a rapid progression to liver damage. Specifically, NK cell population dysregulation of particular interest, as these cells have been shown block HCV replication effectively and an anti-fibrogenic activity. The NKp30 receptor linked tumor lysis has crucial role during viral infections. In the present study, we determined subpopulations based on CD56 CD16 expression, its isoforms A, B, C, along cytotoxicity molecules in patients HIV/HCV.
Background and rationale for the study. Bacterial translocation is an important triggering factor of infection mortality in cirrhosis. In a rat model using bile duct ligation (BDL), bacterial appears within 24 h after ligation. The dynamic between TH1/TH2/TH17 cytokines integrity colonic mucosa context cirrhosis little known. This study aims to determine link intestinal inflammation cholestasis model. Additionally, alterations mucus layer load were also addressed. Results. detected by...
Several studies have reported the benefits and safety of heterologous vaccination among different approved vaccines; however, there are no specific reports on effects with Ad5-nCoV other vaccines same or technologies. In present study, we evaluated neutralizing antibodies percentage against SARS-CoV-2 in Mexican patients immunized vaccine six months after its application. Moreover, effect a booster dose ChAdOx1-S-Nov-19, Ad26.COV2.S, BNT162b2, mRNA-127 were determined. Our results suggest...
Due to the COVID-19 pandemic, rapid development of vaccines against SARS-CoV-2 has been promoted. BNT162b2 is a lipid-nanoparticle mRNA vaccine with 95% efficacy and most administered globally. Nevertheless, little known about cellular immune response triggered by vaccination behavior over time. Therefore, we evaluated T-cell spike protein neutralization antibodies (nAbs) in naïve previously infected subjects vaccinated BTN162b2. Methods: Forty-six BTN162b2 were included (twenty-six twenty...
Developing countries have reported lower molecular diagnostic testing levels due to a lack of resources. Therefore, antibody tests represent an alternative detect exposure SARS-CoV-2 and analyze possible risk factors. We aimed describe compare the clinical-epidemiological characteristics quality food intake in Mexican individuals with positive or negative test antibodies against SARS-CoV-2. carried out applied survey 1799 individuals; 42% were positive, diabetes was more prevalent these...
The NKp30 receptor is one of the three natural cytotoxic receptors reported in NK cells. This codified by NCR3 gene, which encodes isoforms, a consequence alternative splicing exon 4. A greater expression isoforms (A, B, and C), along with low levels ligand B7H6, has been as positive prognostic factor different cancer types. Here, patients cervical precursor lesions, we report an altered immune-phenotype, characterized non-fitness markers, that correlated increased disease stage, from CIN 1...
SARS-CoV-2 variants surveillance is a worldwide task that has been approached with techniques such as Next Generation Sequencing (NGS); however, this technology not widely available in developing countries because of the lack equipment and limited funding science. An option to deploy RT-qPCR screening test which aids analysis higher number samples, shorter time at lower cost. In study, present samples positive for were identified mutation kit later confirmed by NGS. A sample an abnormal...