A5054781121- Gut microbiota and health
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- SARS-CoV-2 and COVID-19 Research
- Diet and metabolism studies
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Dialysis and Renal Disease Management
- Salivary Gland Disorders and Functions
- Intensive Care Unit Cognitive Disorders
- Animal Virus Infections Studies
- COVID-19 diagnosis using AI
- Immunotherapy and Immune Responses
- Hormonal and reproductive studies
- Influenza Virus Research Studies
- Nutrition and Health in Aging
- Pharmacological Effects and Toxicity Studies
- Listeria monocytogenes in Food Safety
- Diabetes and associated disorders
- Viral Infections and Vectors
- HIV-related health complications and treatments
- Ovarian function and disorders
- Oral microbiology and periodontitis research
- Respiratory viral infections research
Universidad de Guadalajara
2020-2024
Yonsei University
2022
Background Several variants of the SARS-CoV-2 have been documented globally during current COVID-19 pandemic. The N501Y, 69-70del, K417N, and E484K mutations among most relevant due to their potential pathogenic biological effects. This study aimed design, validate, propose a fast real-time RT-qPCR assay detect with possible clinical epidemiological relevance in Mexican population. Methods Targeting spike ( S ) gene (N501Y, E484K), specific primers, probes for three quantitative reverse...
Gut microbiota undergoes profound alterations in alcohol cirrhosis. Microbiota-derived products, e.g., short chain fatty acids (SCFA), regulate the homeostasis of gut-liver axis. The objective was to evaluate composition and functions intestinal patients with alcohol-decompensated Fecal samples 18 healthy controls (HC) were obtained. Microbial characterized by 16S rRNA amplicon sequencing, SCFA quantification performed gas chromatography (GC), metagenomic predictive profiles analyzed...
Abstract Alcohol use disorder is a major cause of morbidity, which requires newer treatment approaches. We previously showed in randomized clinical trial that alcohol craving and consumption reduces after fecal transplantation. Here, to determine if this could be transmitted through microbial transfer, germ-free male C57BL/6 mice received stool or sterile supernatants collected from the participants pre-/post-fecal transplant. found colonized with post-fecal transplant but not reduced...
Introduction The variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been classified into interest (VOIs) or concern (VOCs) to prioritize global monitoring and research on with potential risks public health. SARS-CoV-2 high-rate mutation can directly impact the clinical disease progression, epidemiological behavior, immune evasion, vaccine efficacy, transmission rates. Therefore, surveillance is crucial for controlling COVID-19 pandemic. In present study, we aimed...
SARS-CoV-2 has become a global pandemic due to its capacity for rapid transmission. In this context, an early and diagnosis of infected patients that do not require expensive equipment or highly trained personnel is crucial in order reduce the contagious rate. The aim study was evaluate chromatographic immunoassay's performance SARS-CoV-antigen.
Cirrhosis is complicated by a high rate of nosocomial infections (NIs), which result in poor outcomes and are challenging to predict using clinical variables alone. Our aim was determine predictors NI admission serum metabolomics gut microbiota inpatients with cirrhosis. In this multicenter inpatient cirrhosis study, collected on for liquid chromatography-mass spectrometry metabolomics, subset provided stool 16SrRNA analysis. Hospital course, including development death, were analyzed....
Gut microbiota disturbances may influence cognitive function, increasing uremic toxins and inflammation in dialysis patients; therefore, we aimed to evaluate the association of gut profile with impairment (CI) patients on automated peritoneal (APD). In a cross-sectional study, function was evaluated using Montreal Cognitive Assessment 39 APD classified as normal CI. The analyzed 16S rRNA gene sequencing approach. All had clinical, biochemical urea clearance evaluations. Eighty-two percent...
A remarkable increase in metabolic syndrome (MetS) has occurred HIV-infected subjects. Gut dysbiosis is involved the pathogenesis of disorders. Therefore, aim to explore profile gut microbiota Mexican population with HIV infection and MetS.In all, 30 patients MetS were compared a group without MetS, treated integrase inhibitors undetectable viral load included study. Stool samples analysed by 16S rRNA next-generation sequencing. High-sensitivity C-reactive protein >3 mg L-1 higher scores...
Objective First decompensation development is a critical milestone that needs to be predicted. Transkingdom gut microbial interactions, including archaeal methanogens, may important targets and predictors but longitudinal approach needed. Design Cirrhosis outpatients who provided stool twice were included. Group 1: compensated, group 2: 1 (decomp), 3: >1 decompensationwere followed divided into those remained stable or decompensated. Bacteria, viral presence, α/β diversity taxa changes...
Aim Intestinal dysfunction in cirrhosis patients is linked to death by bacterial infections. Currently, there no effective therapy for this complication. This study aims evaluate butyrate, a novel postbiotic, on the intestinal inflammatory response, tight junction proteins and microbiota cholestasis model. Methods Results Wistar rats underwent 15 days of bile duct ligation (BDL). We administered butyrate at concentration 1%. The BDL group did not receive treatment. results showed that could...
Abstract Background and Aims Gut microbiota, including bacteria phages, are altered in cirrhosis, but their role during infections spontaneous bacterial peritonitis (SBP) prophylaxis is unclear. Our aim was determine metagenomic changes gut bacteria; phages linkages centered around Gram‐negative Gram‐positive pathobionts patients with cirrhosis with/without or SBP prophylaxis. Approach Results We included uninfected ( n = 231) infected 30, 19 urinary tract infection 11 before antibiotics)...
Introduction Respiratory viral infections represent a significant global health burden. Historically, influenza, rhinovirus, respiratory syncytial virus, and adenovirus have been the prevalent viruses; however, landscape shifted with widespread emergence of SARS-CoV-2. The aim this study is to present comprehensive epidemiological analysis in Jalisco, Mexico. Methods Data encompassing individuals flu-like symptoms from July 2021 February 2023 was scrutinized for diagnosis through PCR...
Microbial community control is crucial for maintaining homeostasis of the gut–liver axis in metabolic dysfunction-associated steatotic liver disease (MASLD). Here, we show that supplementation with a mixture Mexican foodstuffs (MexMix)—Opuntia ficus indica (nopal), Theobroma cacao (cocoa) and Acheta domesticus (crickets)—enriches several beneficial taxa MASLD mice overweight/obese humans. Thus, MexMix induces an important prebiotic effect. In mice, restoration intestinal health was observed...
Abstract Background and Aims Cirrhosis is associated with changes in gut microbiota both saliva stool. The relative linkage patterns of stool versus systemic metabolomics are unclear may differ across countries. We hypothesized that have greater linkages plasma metabolites than microbiota, which depend on country origin. Methods Age‐balanced controls outpatient patients cirrhosis, compensated decompensated, from the USA Mexico (MX) underwent collection dietary recall. Plasma were analysed...
Due to the COVID-19 pandemic, rapid development of vaccines against SARS-CoV-2 has been promoted. BNT162b2 is a lipid-nanoparticle mRNA vaccine with 95% efficacy and most administered globally. Nevertheless, little known about cellular immune response triggered by vaccination behavior over time. Therefore, we evaluated T-cell spike protein neutralization antibodies (nAbs) in naïve previously infected subjects vaccinated BTN162b2. Methods: Forty-six BTN162b2 were included (twenty-six twenty...
The NKp30 receptor is one of the three natural cytotoxic receptors reported in NK cells. This codified by NCR3 gene, which encodes isoforms, a consequence alternative splicing exon 4. A greater expression isoforms (A, B, and C), along with low levels ligand B7H6, has been as positive prognostic factor different cancer types. Here, patients cervical precursor lesions, we report an altered immune-phenotype, characterized non-fitness markers, that correlated increased disease stage, from CIN 1...
Proton pump inhibitors (PPIs) modulate the progression of cirrhosis to hepatic encephalopathy (HE) and can affect bacterial microbiome. However, impact PPI on virome in using viral-like particle (VLP) analysis is unclear.
SARS-CoV-2 variants surveillance is a worldwide task that has been approached with techniques such as Next Generation Sequencing (NGS); however, this technology not widely available in developing countries because of the lack equipment and limited funding science. An option to deploy RT-qPCR screening test which aids analysis higher number samples, shorter time at lower cost. In study, present samples positive for were identified mutation kit later confirmed by NGS. A sample an abnormal...
Obesity is a public health problem with growing prevalence worldwide. In Mexico, it estimated that one out of three adults suffer from obesity. these patients, the intestinal microbiota (IM) undergoes pathological changes are associated dysbiotic state; however, profile adult subjects obesity western Mexico has not been described. To assess this, fecal samples were obtained 65 participants (Obese = 38; Control 27). The microbial composition was characterized by 16S rRNA amplicon sequencing....
This study aimed to summarize the epidemiological and clinical characteristics of COVID-19 from Western Mexico people during 2020. A retrospective analysis an electronic database visiting a sentinel center for molecular SARS-CoV-2 confirmatory diagnosis by RT-PCR April December 2020 was carried out description COVID-19. Out 23,211 patients evaluated, 6918 (29.8%) were confirmed infection (mean age 38.5 ± 13.99), mostly females (53.8%). Comorbidities, such as diabetes (34.7%), obesity...
Polycystic ovary syndrome (PCOS) is the main endocrine disorder in women of reproductive age worldwide. This condition often associated with various metabolic alterations that contribute to development (MetS). Recent research suggests branched-chain amino acid (BCAA) dysregulation observed PCOS. study aims investigate relationship between dietary patterns, body composition, analytes, and serum levels Mexican Utilizing a cross-sectional design, we found both groups, PCOS (n = 24) + MetS 21),...
Abstract Background and Aims Cognitive impairment is very common in dialysis patients, with negative effects on quality of life mortality. In neurodegenerative conditions (Alzheimer, Parkinson) a gut-brain axis has been identified; however, there no information about the relationship gut microbiota alterations presence cognitive chronic kidney disease. The aim was to associate profile function patients automated peritoneal (APD). Method Cross-sectional study 39 APD patients; those visual or...
Alterations in the intestinal microbiota decompensated cirrhosis are recognized as being critical clinical evolution. The onset of hepatic encephalopathy (HE) worsens prognosis. Metabolic functions related to microbiota, such ammonia production and imbalance amino acid biosynthesis, believed play a key role on pathophysiology HE. This study aimed evaluate composition patients with Fecal samples from 31 cirrhotic (20 HE, 11 without HE) 18 age-balanced healthy controls (HC) were included....