A5083215991- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Liver Disease Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Reproductive System and Pregnancy
- Cervical Cancer and HPV Research
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Liver Disease and Transplantation
- MicroRNA in disease regulation
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Cholinesterase and Neurodegenerative Diseases
- Microscopic Colitis
- Corneal Surgery and Treatments
- Advanced biosensing and bioanalysis techniques
- Spondyloarthritis Studies and Treatments
- Hepatitis B Virus Studies
- Biosensors and Analytical Detection
- Garlic and Onion Studies
- IL-33, ST2, and ILC Pathways
- COVID-19 and Mental Health
Universidad de Guadalajara
2015-2024
Mexican Social Security Institute
2010-2011
University of California, Santa Cruz
2007-2008
Abstract Breast cancer is the most frequent in women worldwide, and drug resistance common all breast types. The combination of natural products with chemotherapies has attracted attention, as it was found that compounds enhance effects standard chemotherapeutic drugs protect from side effects. Into different products, garlic been recognized for its antitumor properties. It suggested anticancer are associated organo‐sulfur compounds, especially alliin allicin. Here, we evaluated both...
B7-H6 has been revealed as an endogenous immunoligand expressed in a variety of tumors, but not healthy tissues. Heretofore, no studies have reported describing women with cervical cancer. To investigate this question, our present study was conducted.This retrospective comprised total 62 paraffinized biopsies, which were distributed five groups: low-grade squamous intraepithelial lesions (LSIL), high-grade (HSIL), carcinoma (SCC), uterine adenocarcinoma (UCAC), and group cervicitis (as...
Background/Aims. Prostate cancer (PCa) is one of the neoplasms with highest incidence and mortality rate in men worldwide. Advanced stages disease are usually very aggressive, most treated chemotherapeutic drugs that generally cause side effects these patients. However, additional therapeutic targets such as IL6R/STAT-3 axis TIGIT have been proposed, mainly due to their relevance development PCa regulation NK cell-mediated cytotoxicity. Here, we evaluate effect inhibitors directed against...
Immune checkpoint therapy to reverse natural killer (NK) and T cell exhaustion has emerged as a promising treatment in various cancers. While anti-programmed death 1 (PD-1) pembrolizumab recently gained Food Drug Administration (FDA) approval for use recurrent or metastatic cervical cancer, other molecules, such immunoreceptor with immunoglobulin (Ig) tyrosine-based inhibition motif (ITIM) domains (TIGIT) mucin-domain containing-3 (Tim-3), have yet be fully explored this disease. We report...
Endosulfan (ENDO) is a widely used organochlorine (OC) pesticide and persistent organo-pollutant. Epidemiological studies have shown that high levels of OC exposure were related to colorectal cancer (CRC) incidence. The objectives the present study evaluate histological changes in colon, as well situ expression β-catenin P-selectin, serum select pro-inflammatory cytokines mice administered ENDO; there relationship between increased IL-6 P-selectin CRC patients aberrant signaling important...
Natural killer (NK) cells are an important resource of the innate immune system directly involved in spontaneous recognition and lysis virus-infected tumor cells. An exquisite balance inhibitory activating receptors tightly controls NK cell activity. At present, one best-characterized is NKG2D, which promotes NK-mediated target by binding to a family surface ligands encoded MHC class I chain-related (MIC) genes, among others. The goal this study was describe expression pattern MICA MICB at...
NKG2D, an activating immunoreceptor, is primarily restricted to NK cells and CD8+ T cells. The existence of atypical cytotoxic CD4+NKG2D+ cell population has also been found in patients with autoimmune dysfunctions. Nonetheless, contradictory evidence categorized this a regulatory rather than role other situations. These confounding data have led the proposal that two distinct subsets might exist. immune response elicited cervical cancer characterized by apparent contradictions concerning...
Abstract Background Although great progress has been made in treatment regimens, cervical cancer remains as one of the most common women worldwide. Studies focusing on molecules that regulate carcinogenesis may provide potential therapeutic strategies for cancer. B7-H6, an activating immunoligand expressed by several tumor cells, is known to activate NK cell-mediated cytotoxicity once engaged with its natural receptor NKp30. However, opposite, is, effects cell triggered B7-H6 after...
Background and rationale for the study. Bacterial translocation is an important triggering factor of infection mortality in cirrhosis. In a rat model using bile duct ligation (BDL), bacterial appears within 24 h after ligation. The dynamic between TH1/TH2/TH17 cytokines integrity colonic mucosa context cirrhosis little known. This study aims to determine link intestinal inflammation cholestasis model. Additionally, alterations mucus layer load were also addressed. Results. detected by...
Abstract Background The NKG2D receptor confers important activating signals to NK cells via ligands expressed during cellular stress and viral infection. This has generated great interest because not only is it on cells, but also seen in virtually all CD8 + cytotoxic T classically considered absent CD4 cells. However, recent studies have identified a distinctive population of that do express NKG2D, which could represent particular effector involved infections chronic diseases. On the other...
Endosulfan is a DDT-era organochlorine pesticide. Due to past and current environmental contamination, investigation of endosulfan exposure importance. Acute high dose precipitates neural/endocrine system damage, but the effects on immune lower doses are not well-characterized. Two relatively low concentrations (i.e. 0.1 17 µM ENDO) were investigated in an vitro study using human peripheral blood mononuclear cells (PBMC) understand (0.1–25.0 [≈0.04–10 ppm/40–10,000 ppb]) ENDO upon normal T-...
Objectives: Exosomes are the smallest of extracellular vesicles and can contain a variety different cargos, including nucleic acids, lipids, proteins. Ultracentrifugation followed by electron microscopy has historically been used for isolation visualization exosomes; Western blot ELISA have also used, but these techniques only semiquantitative unable to distinguish exosome markers in same sample. To resolve some issues, we propose modification bead-based flow cytometry method. Methods:...
Abstract Aims To evaluate the composition and functions of gut microbiota in patients with decompensated alcohol-associated cirrhosis, without hepatic encephalopathy (HE). Methods results Faecal samples from 31 inpatients (20 HE, 11 HE), 18 age-balanced healthy controls (HC), were included. Microbial was determined by 16S rRNA amplicon sequencing analysed using QIIME2. Metabolic pathways inferred PICRUSt2, short-chain fatty acids (SCFAs) quantification performed gas chromatography. The HE...
The NKp30 receptor is one of the three natural cytotoxic receptors reported in NK cells. This codified by NCR3 gene, which encodes isoforms, a consequence alternative splicing exon 4. A greater expression isoforms (A, B, and C), along with low levels ligand B7H6, has been as positive prognostic factor different cancer types. Here, patients cervical precursor lesions, we report an altered immune-phenotype, characterized non-fitness markers, that correlated increased disease stage, from CIN 1...
A streamlined method of skin grafting in mice is described. This procedure eliminates bandages, sutures, and dressings. The elimination bandages renders the overall fast easy to learn. More importantly, bandaging results an increased survival engrafted a drastic reduction graft displacement. Finally lack also makes it possible monitor even earliest stages after engraftment.
Las células NK son parte del sistema inmune, capaces de eliminar dañadas o malignas. Son fundamentales en la detección y eliminación tumores, siendo primera defensa contra su desarrollo. inmunoterapias aprovechan estas para tratar el cáncer. Se investigan estrategias utilizar las manera específica segura tumoral. herramientas biológicas prometedoras lucha cáncer, ofreciendo mejores perspectivas a los pacientes.
CMTM6 is a membrane protein that acts as regulator of PD-L1, maintaining its expression on the cell surface, and can prevent lysosome-mediated degradation. It unknown if present in plasma patients with cervical cancer, it has non-canonical subcellular localizations lines derived from cancer. Our objective was to determine whether found cancer localization lines. Patient separated into exosome-enriched, exosome-free, total fractions. The levels each fraction were determined using ELISA...