Luis Fernando García-Ortega

ORCID: 0000-0002-2021-7165
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About
Contact & Profiles
Research Areas
  • Fermentation and Sensory Analysis
  • Plant Stress Responses and Tolerance
  • Plant Molecular Biology Research
  • Yeasts and Rust Fungi Studies
  • Antifungal resistance and susceptibility
  • Fungal Biology and Applications
  • Fungal Infections and Studies
  • Plant Genetic and Mutation Studies
  • Plant-Microbe Interactions and Immunity
  • Plant Gene Expression Analysis
  • Fungal and yeast genetics research
  • Horticultural and Viticultural Research
  • Legume Nitrogen Fixing Symbiosis
  • Nematode management and characterization studies
  • Probiotics and Fermented Foods
  • Microbial Metabolites in Food Biotechnology
  • Plant pathogens and resistance mechanisms
  • Polyamine Metabolism and Applications
  • Seed and Plant Biochemistry
  • Genomics and Phylogenetic Studies
  • Plant Pathogens and Fungal Diseases
  • Molecular Biology Techniques and Applications
  • Plant biochemistry and biosynthesis
  • Food Chemistry and Fat Analysis
  • Natural Antidiabetic Agents Studies

Center for Research and Advanced Studies of the National Polytechnic Institute
2015-2025

Instituto Tecnológico Superior de Irapuato
2021-2024

Instituto Politécnico Nacional
2015-2023

Institute for Scientific and Technological Research
2018-2021

The American cranberry ( Vaccinium macrocarpon Ait.) is an iconic North fruit crop of great cultural and economic importance. Cranberry can be considered a model due to its unique nutrient composition, overlapping generations, recent domestication, both sexual asexual reproduction modes, the existence cross-compatible wild species. Development molecular resources started very recently; however, further genetic studies are now being limited by lack high-quality genome assembly. Here, we...

10.3389/fpls.2021.633310 article EN cc-by Frontiers in Plant Science 2021-02-10

The environmental deterioration produced by heavy metals derived from anthropogenic activities has gradually increased. worldwide dissemination of toxic in crop soils represents a threat for sustainability and biosafety agriculture requires strategies the recovery metal-polluted soils. biorestoration using technologies that combine plants microorganisms gained attention recent decades due to beneficial synergistic effects its biotic interactions. In this context, native metal-resistant plant...

10.1128/spectrum.00656-22 article EN cc-by Microbiology Spectrum 2022-08-18

RNA-seq experiments estimate the number of genes expressed in a transcriptome as well their relative frequencies. However, an undetermined can remain undetected due to low expression sample size (sequence depth). Estimation true is essential order determine which are exclusively specific tissues or under particular conditions. A reliable also required accurately measure changes and predict sequencing depth needed increase proportion detected genes. This problem analogous ecological sampling...

10.1371/journal.pone.0130262 article EN cc-by PLoS ONE 2015-06-24

Abstract Yeasts are a diverse group of fungal microorganisms that widely used to produce fermented foods and beverages. In Mexico, open fermentations obtain spirits from agave plants. Despite the prevalence this traditional practice throughout country, yeasts have only been isolated studied limited number distilleries. To systematically describe diversity yeast species fermentations, here we generate YMX‐1.0 culture collection by isolating 4524 strains 68 sites with climatic, geographical,...

10.1002/yea.3913 article EN cc-by Yeast 2023-12-06

Epigenetic regulation is a key component of stress responses, acclimatization and adaptation processes in plants. DNA methylation stable mark plausible for the inheritance epigenetic traits, such that it potential scheme plant breeding. However, effect modulators as hydrogen peroxide (H2O2), methylome status has not been elucidated. A transgenic tobacco model to CchGLP gene displayed high H2O2 endogen levels correlated with biotic abiotic stresses resistance. The present study aimed...

10.3390/plants10010178 article EN cc-by Plants 2021-01-19

The ascomycetous yeast Kazachstania humilis is an active species in backslopped sourdough and the spontaneous fermentation of several traditional foods beverages. Here, we report draft genome sequence a

10.1128/mra.01154-21 article EN Microbiology Resource Announcements 2022-03-02

ABSTRACT Yeasts are a diverse group of fungal microorganisms that widely used to produce fermented foods and beverages. In Mexico, open fermentations obtain spirits from agave plants. Despite the prevalence this traditional practice throughout country, yeasts have only been isolated studied limited number distilleries. To systematically describe diversity yeast species fermentations, here we generate YMX.1.0 culture collection by isolating 4,524 strains 68 sites in climatic, geographical,...

10.1101/2023.07.02.547337 preprint EN cc-by-nc bioRxiv (Cold Spring Harbor Laboratory) 2023-07-03

Candida maltosa is closely related to important pathogenic species, especially C. tropicalis and albicans, but it has been rarely isolated from humans. For this reason, through comparative studies, could be a powerful model understand the genetic underpinnings of pathogenicity species. Here, we generated cohesive assembly genome developed engineering tools that will facilitate studying species at molecular level. We used combination short long-read sequencing build polished genomic draft...

10.1099/mic.0.001447 article EN Microbiology 2024-03-08

From yeasts to humans, introgressive hybridization significantly influences the evolutionary history of living organisms by introducing new genetic diversity. Strains Saccharomyces cerevisiae worldwide exhibit introgressions from sister species S. paradoxus , despite average sequence identity between these being lower than 90%. While isolates Neotropics are known for their high levels introgression, events leading unusually prevalence them remain unclear. Here, we sequenced 216 in open agave...

10.1101/2024.09.27.615306 preprint EN cc-by-nc bioRxiv (Cold Spring Harbor Laboratory) 2024-09-28

SARS-CoV-2 variants surveillance is a worldwide task that has been approached with techniques such as Next Generation Sequencing (NGS); however, this technology not widely available in developing countries because of the lack equipment and limited funding science. An option to deploy RT-qPCR screening test which aids analysis higher number samples, shorter time at lower cost. In study, present samples positive for were identified mutation kit later confirmed by NGS. A sample an abnormal...

10.3390/ijms23063143 article EN International Journal of Molecular Sciences 2022-03-15

Polyamines are organic and aliphatic molecules essential for the growth, development, survival of both eukaryotes prokaryotes. In fungi, polyamines play a crucial role in cellular differentiation pathogenesis. Since fungi animals closely related evolutionarily, can be easily genetically manipulated lab, they serve as excellent models studying polyamine metabolism molecular mechanisms controlled by these biomolecules. Although has been extensively studied model such Saccharomyces cerevisiae...

10.1002/jobm.202400561 article EN Journal of Basic Microbiology 2024-11-11

Candida dubliniensis is the most closely related species to C. albicans , one of leading causes fungal infections in humans. However, despite sharing many characteristics, significantly less pathogenic. To better understand molecular underpinnings these dissimilarities, we focused on regulation filamentation, a developmental trait fundamental for host colonization. We generated collection 44 null mutants transcription regulators whose orthologs had been previously implicated filamentous...

10.1101/2024.11.20.624441 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2024-11-20

Plants require effective sensing and responses to environmental stimuli, for example in the case of biotic abiotic stresses. Reactive oxygen species (ROS) are well-recognised key signal molecules stress response. In previous work our group, several transgenic tobacco lines were generated constitutively expressing a germin-like gene from Capsicum chinense (CchGLP), producing higher endogen levels hydrogen peroxide that correlated with tolerance geminivirus infections drought stress....

10.1080/14620316.2017.1421485 article EN The Journal of Horticultural Science and Biotechnology 2018-01-12
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