A5020928977- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- Advanced Glycation End Products research
- Apelin-related biomedical research
- Rheumatoid Arthritis Research and Therapies
- Galectins and Cancer Biology
- Autoimmune and Inflammatory Disorders Research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Natural Antidiabetic Agents Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Atherosclerosis and Cardiovascular Diseases
- Gastroesophageal reflux and treatments
- Osteoarthritis Treatment and Mechanisms
- Systemic Lupus Erythematosus Research
- Lanthanide and Transition Metal Complexes
- Glycosylation and Glycoproteins Research
- Alzheimer's disease research and treatments
- Chronic Kidney Disease and Diabetes
- Medical Imaging and Pathology Studies
- Biochemical effects in animals
- Antioxidant Activity and Oxidative Stress
- GDF15 and Related Biomarkers
- Systemic Sclerosis and Related Diseases
- Metabolism, Diabetes, and Cancer
- Pneumocystis jirovecii pneumonia detection and treatment
Yale University
2016-2025
Yale Cancer Center
2011-2025
American College of Rheumatology
2018-2024
Manitoba Beekeepers' Association
2024
Cedars-Sinai Medical Center
2023
Johnson University
2023
Rutgers, The State University of New Jersey
2012-2023
Rutgers Sexual and Reproductive Health and Rights
2023
Fairview Southdale Hospital
2023
Cleveland Clinic
2023
Nitric oxide (an endothelium-derived relaxing factor) induces smooth muscle relaxation and is an important mediator in the regulation of vascular tone. Advanced glycosylation end products, glucose-derived moieties that form nonenzymatically accumulate on long-lived tissue proteins, have been implicated many complications diabetes normal aging. We demonstrate advanced products quench nitric activity vitro vivo. Acceleration process vivo results a time-dependent impairment...
Abstract Fibrocytes are a distinct population of blood-borne cells that display unique cell surface phenotype (collagen I+/CD11b+/CD13+/CD34+/CD45RO+/MHC class II+/CD86+) and exhibit potent immunostimulatory activities. Circulating fibrocytes rapidly enter sites tissue injury, suggesting an important role for these in wound repair. However, the regulatory processes govern differentiation mechanisms underlie migration to currently not known. We report herein ex vivo cultured can differentiate...
Alzheimer disease (AD) is characterized by deposits of an aggregated 42-amino-acid beta-amyloid peptide (beta AP) in the brain and cerebrovasculature. After a concentration-dependent lag period during vitro incubations, soluble preparations synthetic beta AP slowly form fibrillar aggregates that resemble natural amyloid are measurable sedimentation thioflavin T-based fluorescence. Aggregation these assays enhanced addition small amounts pre-aggregated "seed" material. We also have prepared...
Endogenous advanced glycation endproducts (AGEs) include chemically crosslinking species (glycotoxins) that contribute to the vascular and renal complications of diabetes mellitus (DM). Renal excretion catabolic products endogenous AGEs is impaired in patients with diabetic or nondiabetic kidney disease (KD). The aim this study was examine oral absorption clearance kinetics food DM KD whether circulating diet-derived contain active glycotoxins. Thirty-eight diabetics (DM) without five...
The protein known as macrophage migration inhibitory factor (MIF) was one of the first cytokines to be discovered and described 30 years ago a T-cell-derived that inhibited random macrophages in vitro. A much broader role for MIF has emerged recently result studies have demonstrated it released from anterior pituitary gland vivo. also is been identified secreted monocytes/macrophages upon glucocorticoid stimulation. Once released, acts "override" or counter-regulate suppressive effects...
The importance of the macrophage in innate immunity is underscored by its secretion an array powerful immunoregulatory and effector molecules. We report herein that migration inhibitory factor (MIF), a product activated macrophages, sustains survival function suppressing activation-induced, p53-dependent apoptosis. Endotoxin administration to MIF −/− mice results decreased viability, proinflammatory function, increased apoptosis when compared with wild-type controls. Moreover, inhibition p53...
To address potential mechanisms for oxidative modification of lipids in vivo, we investigated the possibility that phospholipids react directly with glucose to form advanced glycosylation end products (AGEs) then initiate lipid oxidation. Phospholipid-linked AGEs formed readily vitro, mimicking absorbance, fluorescence, and immunochemical properties result from proteins. Oxidation unsaturated fatty acid residues, as assessed by reactive aldehyde formation, occurred at a rate paralleled...
Smokers have a significantly higher risk for developing coronary and cerebrovascular disease than nonsmokers. Advanced glycation end products (AGEs) are reactive, cross-linking moieties that form from the reaction of reducing sugars amino groups proteins, lipids, nucleic acids. AGEs circulate in high concentrations plasma patients with diabetes or renal insufficiency been linked to accelerated vasculopathy seen these diseases. Because curing tobacco takes place under conditions could lead...
Atherosclerosis develops rapidly in patients with diabetes or renal insufficiency. Plasma lipoprotein profiles are frequently abnormal these conditions and reflect an elevation the level of apoprotein B (ApoB)-containing components very low density (VLDL) (LDL). High levels circulating advanced glycation end products (AGEs) also occur end-stage disease (ESRD). These arise from glucose-derived Amadori include AGE-modified peptides (AGE-peptides) which result catabolism tissue proteins....
Advanced glycation end products (AGEs) are linked with the development of diabetic retinopathy; however, pathogenic mechanisms poorly defined. Vascular endothelial growth factor (VEGF) levels increased in ischemic and nonischemic retina, VEGF is required for retinal iris neovascularization. Moreover, alone can induce much concomitant pathology retinopathy. In this study, we found that AGEs mRNA ganglion, inner nuclear, pigment epithelial (RPE) cell layers rat retina. vitro, secreted protein...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification, Bioactivity, and Secondary Structure Analysis of Mouse Human Macrophage Migration Inhibitory Factor (MIF)Jurgen Bernhagen, Robert A. Mitchell, Thierry Calandra, Wolfgang Voelter, Anthony Cerami, Richard BucalaCite this: Biochemistry 1994, 33, 47, 14144–14155Publication Date (Print):November 1, 1994Publication History Published online1 May 2002Published inissue 1 November...
Advanced glycosylation end products (AGEs) have been implicated in many of the complications diabetes and normal aging. Markedly elevated vascular tissue circulating AGEs were linked recently to accelerated vasculopathy end-stage diabetic renal disease. To determine pathogenic role vivo, AGE-modified albumin was administered healthy nondiabetic rats rabbits alone or combination with AGE-crosslink inhibitor aminoguanidine. Within 2-4 weeks AGE treatment, content aortic samples rose six times...