A5037287374- Macrophage Migration Inhibitory Factor
- Advanced Glycation End Products research
- Nuclear Receptors and Signaling
- Cholesterol and Lipid Metabolism
- Biochemical effects in animals
- Antimicrobial Peptides and Activities
- Chemical Synthesis and Analysis
- Crystallization and Solubility Studies
- Peroxisome Proliferator-Activated Receptors
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- X-ray Diffraction in Crystallography
- Hemoglobin structure and function
- Biochemical Analysis and Sensing Techniques
- SARS-CoV-2 and COVID-19 Research
- Inflammatory mediators and NSAID effects
- Click Chemistry and Applications
- Regulation of Appetite and Obesity
- Receptor Mechanisms and Signaling
- Drug Transport and Resistance Mechanisms
- Pulmonary Hypertension Research and Treatments
- Synthesis of heterocyclic compounds
- Electron Spin Resonance Studies
- Alcohol Consumption and Health Effects
- COVID-19 Clinical Research Studies
Feinstein Institute for Medical Research
2010-2023
Northwell Health
2011-2022
Albert Einstein College of Medicine
2001-2004
Institute for Medical Research
2001
University of Aberdeen
1999
Smokers have a significantly higher risk for developing coronary and cerebrovascular disease than nonsmokers. Advanced glycation end products (AGEs) are reactive, cross-linking moieties that form from the reaction of reducing sugars amino groups proteins, lipids, nucleic acids. AGEs circulate in high concentrations plasma patients with diabetes or renal insufficiency been linked to accelerated vasculopathy seen these diseases. Because curing tobacco takes place under conditions could lead...
The scavenger receptor class B type I (SR-BI), which is expressed in the liver and intestine, plays a critical role cholesterol metabolism rodents. While hepatic SR-BI expression controls high density lipoprotein (HDL) metabolism, intestinal has been proposed to facilitate absorption. To evaluate further relevance of enterohepatic circulation bile salts, we studied biliary lipid secretion, sterol content synthesis, acid fecal neutral excretion, absorption knockout mice. deficiency...
During a disease outbreak/pandemic situation such as COVID-19, researchers are in prime position to identify and develop peptide-based therapies, which could be more rapidly cost-effectively advanced into clinical setting. One drawback of natural peptide drugs, however, is their proteolytic instability; peptidomimetics can help overcome this caveat. In review, we summarize therapeutics that target one main entry pathway SARS-CoV-2, involves the host ACE2 receptor viral spike (S) protein...
Human obesity is associated with elevated plasma leptin levels. Obesity also an important risk factor for cholesterol gallstones, which form as a result of hypersecretion into bile. Because levels are correlated gallstone prevalence, we explored the effects acute administration on biliary secretion using lean ( FA/−) and obese fa/fa) Zucker rats. rats become hyperleptinemic due to homozygosity missense mutation in receptor, diminishes but does not completely eliminate responsiveness leptin....
Abnormally low plasma concentrations of thyroid hormones during sepsis often occur in the absence thyroidal illness; however, mechanisms involved “euthyroid sick syndrome” remain poorly understood. Here, we describe a previously unrecognized interaction between hormone thyroxine (T 4 ) and proinflammatory cytokine macrophage migration inhibitory factor (MIF), together with its clinical relevance sepsis. We found that both patients severe sepsis, our rodent model, T were inversely correlated...
Peptidomimetics, synthetically modified peptides which have been engineered to eliminate certain undesirable features of native therapeutic including short half-lives, immunogenicity, and delivery challenges, are a growing class peptide-based therapeutics. Azapeptides (αC N substitution(s)), type peptidomimetic with several examples in clinical use, known exhibit increased resistance endogenous proteases, extending plasma half-life, while potentially retaining or enhancing biological...
Supplemental data for this article can be accessed here.High mobility group box-1 (HMGb1), an endogenous danger-associated molecular pattern protein (DAMP) whose extracellular release has been associated with sterile injury and various inflammatory diseases conditions, shown to a valuable clinical drug target. Elucidation of the specific interactions HMGb1 receptor, Toll-like receptor 4 (TLR4) adaptor myeloid differentiation factor-2 (MD-2), will lead more precisely targeted therapeutics. We...
To elucidate the control of hepatic cholesterol metabolism by leptin, rats were administered IV (intravenous) ICV (intracerebroventricular) leptin or saline. A single low dose was as effective a continuous infusion high-dose at decreasing activities 3-hydroxy-3-methylglutaryl-CoA reductase and 7alpha-hydroxylase. These results indicate that response to is transduced via central nervous system.
Typically, a diagnosis of diabetes mellitus is based on elevated circulating blood glucose levels. In an attempt to discover additional markers for the disease and predictors prognosis, we undertook characterization HbA1d3 in diabetic normal patients.PolyCAT A cation exchange chromatography liquid chromatography-mass spectroscopy was utilized separate alpha- beta-globin chains characterize their presence patients.We report as glutathionylated, minor hemoglobin subfraction that occurs higher...
Systemic lupus erythematosus is an autoimmune disease that can affect numerous tissues and characterized by the production of nuclear antigen-directed autoantibodies (e.g., anti-dsDNA). Using a combination virtual ELISA-based screens, we made intriguing discovery several HIV-protease inhibitors function as decoy antigens to specifically inhibit binding anti-dsDNA antibodies target such dsDNA pentapeptide DWEYS. Computational modeling revealed comprised structural features present in DWEYS...
Abstract Pulmonary hypertension ( PH ) is a complex disease comprising pathologic remodeling and thickening of the pulmonary vessels causing an after load on right heart ventricle that can result in ventricular failure. Triggered by oxidative stress, episodes hypoxia, other undetermined causes, associated with poor outcomes high rate morbidity. In neonate, this has similar etiology but further complicated transition to breathing birth, which requires reduction vascular resistance. Persistent...
Background: Cardiac arrest (CA) can lead to neuronal degeneration and death through various pathways, including oxidative, inflammatory, metabolic stress. However, current neuroprotective drug therapies will typically target only one of these most single attempts correct the multiple dysregulated pathways elicited following cardiac have failed demonstrate clear benefit. Many scientists opined on need for novel, multidimensional approaches disturbances after arrest. In study, we developed a...
Dicarbonyl-containing compounds such as methylglyoxal (MG) are toxic to cells since they can interact with the nucleophilic centers of macromolecules. MG has been found accumulate during hyperglycemia, and it suggested that this reactive dicarbonyl may contribute tissue damage long-term complications diabetes. A sensitive bacterial assay for investigating ability agents detoxify developed. This utilizes sensitivity exponential phase an Escherichia coli double mutant lacking KefB KefC...
Macrophage migration inhibitory factor (MIF), a cytokine involved in the pathogenesis of sepsis, diabetes, asthma, arthritis, and cancer, has an enzymatic active site that proven to be effective target for small molecule-based inhibitors. Herein, we describe synthesis novel arylazoarylmethane compound (3) from known arylhydrazone MIF inhibitor (2). This new improved stability vivo biological activity.
Abstract Peptides, polymers of amino acids, comprise a vital and expanding therapeutic approach. Their rapid degradation by proteases, however, represents major limitation to their utility chemical modifications native peptides have been employed mitigate this weakness. Herein, we describe functionalized thiocarbazate scaffolds as precursors aza-amino that, upon activation, can be integrated in peptide sequence generate azapeptides using conventional synthetic methods. This methodology...