A5089227561- Cell Adhesion Molecules Research
- Chemokine receptors and signaling
- Atherosclerosis and Cardiovascular Diseases
- Platelet Disorders and Treatments
- Extracellular vesicles in disease
- Blood Coagulation and Thrombosis Mechanisms
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood properties and coagulation
- Monoclonal and Polyclonal Antibodies Research
- Protease and Inhibitor Mechanisms
- Inflammatory Biomarkers in Disease Prognosis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Liver Disease Diagnosis and Treatment
- Nuclear Receptors and Signaling
- Venous Thromboembolism Diagnosis and Management
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- Liver physiology and pathology
- T-cell and B-cell Immunology
- Galectins and Cancer Biology
- Macrophage Migration Inhibitory Factor
- Cardiac Fibrosis and Remodeling
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
Maastricht University
2016-2025
Imperial College London
2023
Children's Hospital of Philadelphia
2023
University of Pennsylvania
2023
Cardiovascular Institute Hospital
2018-2022
Ludwig-Maximilians-Universität München
2010-2021
Institute for Sports Medicine
2011-2021
RWTH Aachen University
2004-2017
German Centre for Cardiovascular Research
2013-2017
University of Medicine and Pharmacy of Craiova
2017
Macrophages in atherosclerotic plaques drive inflammatory responses, degrade lipoproteins, and phagocytose dead cells. MicroRNAs (miRs) control the differentiation activity of macrophages by regulating signaling key transcription factors. However, functional role macrophage-related miRs immune response during atherogenesis is unknown. Here, we report that miR-155 specifically expressed proinflammatory macrophages, where it was induced treatment with mildly oxidized LDL (moxLDL) IFN-γ....
Inflammation has been closely linked to auto-immunogenic processes in atherosclerosis. Plasmacytoid dendritic cells (pDCs) are specialized produce type-I interferons response pathogenic single-stranded nucleic acids, but can also sense self-DNA released from dying or neutrophil extracellular traps complexed the antimicrobial peptide Cramp/LL37 autoimmune disease. However, exact role of pDCs atherosclerosis remains elusive.Here we demonstrate that be detected murine and human atherosclerotic...
Platelet activation mediates multiple cellular responses, including secretion of chemokines such as RANTES (CCL5), and formation platelet microparticles (PMPs). We studied the role PMPs in delivering promoting monocyte recruitment.Here we show that contain substantial amounts deposit on activated endothelium or murine atherosclerotic carotid arteries. deposition is facilitated by flow conditions more efficient than conferred PMP supernatants. Interactions with were mostly characterized...
Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and EV-free secretome to homeostasis disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates EVs free secreted molecules proteomic functional studies. Conditioned media BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, separation protein using sepharose CL-4B SEC....
Rationale: Acute lung injury (ALI) causes high mortality, but its molecular mechanisms and therapeutic options remain ill-defined. Gram-negative bacterial infections are the main cause of ALI, leading to neutrophil infiltration, permeability increases, deterioration gas exchange, damage. Platelets activated during insights into their mechanistic contribution accumulation in elusive.Objectives: To determine platelet-mediated recruitment ALI.Methods: Interference with platelet–neutrophil...
Activation of hepatic stellate cells in response to chronic inflammation represents a crucial step the development liver fibrosis. However, molecules involved interaction between immune and remain obscure. Herein, we identify chemokine CCL5 (also known as RANTES), which is induced murine human after injury, central mediator this interaction. First, showed patients with fibrosis that haplotypes intrahepatic mRNA expression were associated severe Consistent this, detected Ccl5 protein 2 mouse...
Growth differentiation factor (GDF) 15 is a member of the transforming growth β (TGF-β) superfamily, which operates in acute phase responses through currently unknown receptor. Elevated GDF-15 serum levels were recently identified as risk for coronary syndromes. We show that expression up-regulated disease progresses murine atherosclerosis and primarily colocalizes with plaque macrophages. Hematopoietic deficiency low density lipoprotein receptor−/− mice led to impaired initial lesion...
Research Article18 February 2013Open Access Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment classical monocytes Oliver Soehnlein Corresponding Author [email protected] Institute for Cardiovascular Prevention, Ludwig-Maximilians-University, Munich, Germany Department Pathology, Academic Medical Center Amsterdam, University Netherlands Molecular (IMCAR), RWTH Aachen University, Search more papers by this author Maik Drechsler Yvonne Döring Dirk...
We have recently identified the archaic cytokine macrophage migration inhibitory factor (MIF) as a non-canonical ligand of CXC chemokine receptors CXCR2 and CXCR4 in inflammatory atherogenic cell recruitment. Because its affinity for was particularly high, we hypothesized that MIF may feature structural motives shared by canonical ligands, namely conserved N-terminal Glu-Leu-Arg (ELR) motif. Sequence alignment modeling indeed revealed pseudo-(E)LR motif (Asp-44-X-Arg-11) constituted...
Platelets shed microparticles not only upon activation, but also ageing by an apoptosis-like process (apoptosis-induced platelet microparticles, PMap). While the activation-induced have widely been studied, much is known about (patho)physiological consequences of PMap formation. Flow cytometry and scanning electron microscopy demonstrated that display activated integrins interact to form microparticle aggregates. were chemotactic for monocytic cells, bound these furthermore stimulated cell...
Chemokines orchestrate leukocyte trafficking and function in health disease. Heterophilic interactions between chemokines a given microenvironment may amplify, inhibit, or modulate their activity; however, systematic evaluation of the chemokine interactome has not been performed. We used immunoligand blotting surface plasmon resonance to obtain comprehensive map chemokine-chemokine confirm specificity. Structure-function analyses revealed that activity can be enhanced by CC-type heterodimers...
Chemoattractant cytokines or chemokines constitute a family of structurally related proteins found in vertebrates, bacteria, viruses. So far, 48 chemokine genes have been identified humans, which bind to around 20 receptors. These receptors belong the seven transmembrane G-protein-coupled receptor family. Chemokines and their were originally studied for role cellular trafficking leukocytes during inflammation immune surveillance. It is now known that they exert different functions under...
Background— Junctional adhesion molecule (JAM)-A expressed in endothelial, epithelial, and blood cells can regulate permeability leukocyte extravasation. Atherosclerosis develops at sites of disturbed flow large arteries, but the mechanisms guiding inflammatory into these predilection remain unknown. Methods Results— To characterize cell-specific functions JAM-A atherosclerosis, we used apolipoprotein E–deficient mice with a somatic or endothelium-specific deficiency bone marrow chimeras...
ABSTRACT Extracellular vesicles (EVs) are mediators of cell communication during health and disease, abundantly released by platelets upon activation or ageing. Platelet EVs exert modulatory effects on immune vascular cells. may modulate the function smooth muscle cells (SMC). were isolated from platelet‐rich plasma incubated with SMC in order to assess binding, proliferation, migration pro‐inflammatory phenotype firmly bound resting through platelet integrin α IIb β 3 , while binding also...
The ageing population continues to suffer from its primary killer, cardiovascular disease (CVD). Despite recent advances in interventional medicinal and surgical therapies towards the end of 20th century, epidemic has not been halted. Yet, rather than receding globally, burden CVD risen become a top cause morbidity mortality worldwide. Most arises thrombotic rupture an atherosclerotic plaque, pathologic thickening coronary carotid artery segments subsequent distal ischemia heart or brain. In...