A5061625650- Adipose Tissue and Metabolism
- Diet and metabolism studies
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle metabolism and nutrition
- Regulation of Appetite and Obesity
- Obesity, Physical Activity, Diet
- Lipid metabolism and biosynthesis
- Advanced Proteomics Techniques and Applications
- Nutrition, Genetics, and Disease
- Metabolomics and Mass Spectrometry Studies
- Metabolism, Diabetes, and Cancer
- Peroxisome Proliferator-Activated Receptors
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Pancreatic function and diabetes
- Eating Disorders and Behaviors
- Protease and Inhibitor Mechanisms
- Birth, Development, and Health
- Digestive system and related health
- Drug Transport and Resistance Mechanisms
- Genetic Associations and Epidemiology
- Biochemical Analysis and Sensing Techniques
- Mass Spectrometry Techniques and Applications
- Amino Acid Enzymes and Metabolism
Maastricht University Medical Centre
2014-2024
Maastricht University
2015-2024
Liverpool John Moores University
2020
New York University Press
2016
Cambridge University Press
2016
Hasselt University
2009-2010
Goethe University Frankfurt
2010
Universität Ulm
2010
Transnational University Limburg
2009
Food & Nutrition
2009
Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and EV-free secretome to homeostasis disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates EVs free secreted molecules proteomic functional studies. Conditioned media BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, separation protein using sepharose CL-4B SEC....
<i>Objective:</i> Matrix γ-carboxyglutamate (Gla) protein (MGP) is a vitamin K-dependent and strong inhibitor of vascular calcification. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites arterial We hypothesized that as result ucMGP deposition around calcification, the circulating fraction decreased. Here we report on development an assay potential diagnostic utility monitoring serum levels. <i>Methods Results:</i> An...
Abstract The specificity of proteins identified by proteomics as biomarkers for defined conditions or components biological processes and pathways is crucial. We critically reviewed differentially expressed from comparative proteomic studies 2‐DE followed MS, especially with MALDI technique. Based on 66 those studies, a list 44 presented generally detected regardless species, in vivo vitro conditions, tissues organs, experimental objective. Similarly, 28 protein families presented. enriched...
Unexpected hepatotoxicity is one of the major reasons drugs failing in clinical trials. This emphasizes need for new screening methods that address toxicological hazards early drug discovery process. Here, proteomics techniques were used to gain further insight into mechanistic processes hepatotoxic compounds. Drug-induced mainly divided hepatic steatosis, cholestasis, or necrosis. For each class, a compound was selected, respectively amiodarone, cyclosporin A, and acetaminophen. The changes...
Lactobacillus plantarum is part of the natural microbiota many food fermentations as well human gastro-intestinal tract. The cytosolic fraction proteome L. WCFS1, whose genome has been sequenced, was studied. 2-DE used to investigate proteins from isolated mid- and late-log, early- late-stationary phase cells generate reference maps different growth conditions offering more knowledge metabolic behavior this bacterium. From fraction, a total 200 protein spots were identified by MALDI-MS...
In vitro models are indispensable study objects in the fields of cell and molecular biology, with advantages such as accessibility, homogeneity population, reproducibility, growth rate. The Caco-2 line, originating from a colon carcinoma, is widely used model for small intestinal epithelium. Cancer cells have an altered metabolism, making it difficult to infer their representativity tissue which they derived. This was designed compare protein expression pattern patterns epithelial human...
This study was designed to develop, optimize and validate protocols for blood processing prior proteomic analysis of plasma, platelets peripheral mononuclear cells (PBMC) determine analytical variation a single sample depleted platelet PBMC proteins within between four laboratories each using their own standard operating 2D gel electrophoresis. Plasma either the Beckman Coulter IgY-12 proteome partitioning kit or Amersham albumin IgG depletion columns gave good quality gels, but...
The thiazolidinedione (TZD) rosiglitazone is a peroxisome proliferator-activated receptor-gamma agonist that induces adipocyte differentiation and, hence, lipid accumulation. This in apparent contrast to the long-term glucose-lowering, insulin-sensitising effect of rosiglitazone. We tested whether action involves specific effects on mature adipocytes, which are different from those preadipocytes.Differentiated 3T3-L1 adipocytes were used as an vitro model. Transcriptomics, proteomics and...
Visfatin with the official gene name pre-B cell colony-enhancing factor 1 (PBEF) and protein nicotinamide phosphoribosyltransferase (NAMPT) is a recently discovered adipocyte-secreted that was shown by some to be associated visceral fat insulin resistance. To explore link between PBEF/NAMPT/visfatin lipid metabolism, we analyzed relation of its plasma level several parameters adiposity, resistance circulating blood profile in group general population (n = 40) subjects who are genetically...
Adipose tissue is an endocrine organ secreting different types of proteins, known as adipokines. These adipokines play important roles in homeostasis and metabolism. Adipocyte differentiation leads to a change adipokine secretion profile which probably involved disruption homeostasis. Many have been identified but species differences limitations human adipose material urged the need for better model systems. Here we used cell strain derived from Simpson Golabi Behmel syndrome (SGBS) patient....
The influence of single nucleotide polymorphisms (SNP) the FTO gene has been shown to change over time.The aim was investigate relationship between a SNP (rs9939609) and obesity-related characteristics longitudinally during childhood puberty.From 101 children (58 boys 43 girls), genotype yearly anthropometric data from birth age 7 yr were determined. From ages 12 17 yr, we measured anthropometry, body composition, leptin concentrations, physical activity, hours watching television, attitude...
ABSTRACT Airway epithelial cells secrete extracellular vesicles (EVs) under basal conditions and when exposed to cigarette smoke extract (CSE). Getting insights into the composition of these EVs will help unravel their functions in homeostasis smoking‐induced pathology. Here, we characterized proteomic CSE‐induced airway EVs. BEAS‐2B were left unexposed or 1% CSE for 24 h, followed by EV isolation using ultrafiltration size exclusion chromatography. Isolated labelled with tandem mass tags...
Differential gel electrophoresis followed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is a commonly used protein profiling method. However, observed changes can be explained in multiple ways, one which the turnover rate. In order to easily and rapidly obtain information on both identity individual proteins, we applied combination labeling with L-(ring-2,3,4,5,6 2H5) phenylalanine MALDI-TOF MS. While spectrum reveals protein, isotopomer...
We have applied our recently designed proteomics apparoach to search for protein changes in the vivo adipocyte-enriched proteome from 8 overweight/obese subjects who underwent an intervention of 5 weeks a very low calorie diet followed by 3 normal diet. On average, persons lost 9.5 kg body weight largely contributed loss fat mass (7.1 kg). Various parameters adiposity and lipid metabolism changed significantly. Proteomics analysis revealed 6 significantly proteins. Analysis indicates that...
Background Weight regain after weight loss is common. In the Diogenes dietary intervention study, high protein and low glycemic index (GI) diet improved maintenance. Objective To identify blood predictors for change following within study. Design Blood samples were collected at baseline 8-week caloric diet-induced from 48 women who continued to lose regained during subsequent 6-month period with 4 diets varying in GI levels. Thirty-one proteins 3 steroid hormones measured. Results...
Abstract BACKGROUND Cardiac troponin T (cTnT) is the preferred biomarker for diagnosis of acute myocardial infarction (AMI). It has been suggested that cTnT present predominantly in fragmented forms human serum following AMI. In this study, we have used a targeted mass spectrometry assay and epitope mapping using Western blotting to confirm hypothesis. METHODS was captured from 12 patients diagnosed with AMI an immunoprecipitation technique employing M11.7 catcher antibody fractionated...
Background Short chain fatty acids (SCFA) are increasingly recognized for their potential ability to alleviate obesity-associated chronic low-grade inflammation and disturbed energy homeostasis. Evidence suggests that an increase in circulating SCFA might be necessary induce beneficial alterations metabolism. Objective To compare the bioaccessibility of two different SCFA-enriched triglycerides: Akovita SCT (butyrate hexanoate esterified with long acids) tributyrin/caproin (solely butyrate...