A5087773614- Gut microbiota and health
- Reproductive tract infections research
- Atherosclerosis and Cardiovascular Diseases
- Extracellular vesicles in disease
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Bacterial Infections and Vaccines
- Systemic Lupus Erythematosus Research
- Pneumonia and Respiratory Infections
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Clostridium difficile and Clostridium perfringens research
- Neuropeptides and Animal Physiology
- Nitric Oxide and Endothelin Effects
- Pelvic floor disorders treatments
- Inflammatory Bowel Disease
- MicroRNA in disease regulation
- Herpesvirus Infections and Treatments
- Air Quality and Health Impacts
- Receptor Mechanisms and Signaling
- Cardiac and Coronary Surgery Techniques
- Inflammasome and immune disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammatory Biomarkers in Disease Prognosis
Maastricht University
2012-2024
Maastricht University Medical Centre
2008-2024
University Medical Center
2011-2018
University Hospital and Clinics
2015
Czech Academy of Sciences, Institute of Microbiology
2009
Cardiovascular Institute Hospital
2007
University of Cologne
2007
Appropriate isolation methods are essential for unravelling the relative contribution of extracellular vesicles (EVs) and EV-free secretome to homeostasis disease. We hypothesized that ultrafiltration followed by size exclusion chromatography (UF-SEC) provides well-matched concentrates EVs free secreted molecules proteomic functional studies. Conditioned media BEAS-2B bronchial epithelial cells were concentrated on 10 kDa centrifuge filters, separation protein using sepharose CL-4B SEC....
Cardiac hypertrophy and subsequent heart failure triggered by chronic hypertension represent major challenges for cardiovascular research. Beyond neurohormonal myocyte signaling pathways, growing evidence suggests inflammatory pathways as therapeutically targetable contributors to this process. We recently reported that microRNA-155 is a key mediator of cardiac inflammation injury in infectious myocarditis. Here, we investigated the impact manipulation hypertensive disease.Genetic loss or...
Viral myocarditis results from an adverse immune response to cardiotropic viruses, which causes irreversible myocyte destruction and heart failure in previously healthy people. The involvement of microRNAs their usefulness as therapeutic targets this process are unknown.To identify involved viral pathogenesis susceptibility.Cardiac were profiled both human Coxsackievirus B3-injected mice, comparing myocarditis-susceptible with nonsusceptible mouse strains longitudinally. MicroRNA responses...
Abdominal aortic aneurysms (AAA) are characterized by extensive transmural inflammation and C-reactive protein (CRP) has emerged as an independent risk factor for the development of cardiovascular disease. Therefore, we evaluated a possible association between serum CRP aneurysm dimension in patients with asymptomatic AAA. Furthermore, possibility production aneurysmal tissue been examined.Serum was determined highly sensitive (hsCRP) size measured 39 The presence mRNA assessed 16 patients....
Next-generation sequencing (NGS) has instigated the research on role of microbiome in health and disease. The compositional nature such datasets makes it however challenging to identify those microbial taxa that are truly associated with an intervention or outcome. Quantitative profiling overcomes structure data by integrating absolute quantification abundances into NGS data. Both cell-based methods (e.g. flow cytometry) molecular (qPCR) have been used determine abundances, but what extend...
ABSTRACT Extracellular vesicles (EVs) are mediators of cell communication during health and disease, abundantly released by platelets upon activation or ageing. Platelet EVs exert modulatory effects on immune vascular cells. may modulate the function smooth muscle cells (SMC). were isolated from platelet‐rich plasma incubated with SMC in order to assess binding, proliferation, migration pro‐inflammatory phenotype firmly bound resting through platelet integrin α IIb β 3 , while binding also...
Colonization of the airways with potential pathogenic bacteria is observed in a number chronic respiratory diseases, such as COPD or cystic fibrosis. Infections viruses are known triggers exacerbations these diseases. We here investigated if pre-exposure to alters response lung epithelial cells subsequent viral infection.Bronchial (BEAS-2B and primary bronchial cells) were exposed heat-inactivated Haemophilus influenzae, Pseudomonas aeruginosa Streptococcus pneumoniae subsequently infected...
Nontypeable Haemophilus influenzae (NTHI), a common colonizer of lungs patients with chronic obstructive pulmonary disease (COPD), can enhance expression the cellular receptor intercellular adhesion molecule 1 (ICAM-1), which in turn be used by major group human rhinoviruses (HRVs) for attachment. Here, we evaluated effect NTHI-induced up-regulation ICAM-1 on viral replication and inflammatory responses toward different respiratory viruses. Therefore, bronchial epithelial cells were...
Persistent C. trachomatis infections are assumed to increase the risk of tubal pathology. We studied whether serological markers, be associated with persistent infections, could identify subfertile women at pathology.Sera 313 women, who all underwent a laparoscopy testing assess grade pathology, were tested for presence immunoglobulin (Ig) G and IgA antibodies trachomatis, IgG chlamydia heat shock protein 60 (cHSP60) C-reactive (CRP).C. IgA, cHSP60 CRP, markers significantly more prevalent...
ABSTRACT Airway epithelial cells secrete extracellular vesicles (EVs) under basal conditions and when exposed to cigarette smoke extract (CSE). Getting insights into the composition of these EVs will help unravel their functions in homeostasis smoking‐induced pathology. Here, we characterized proteomic CSE‐induced airway EVs. BEAS‐2B were left unexposed or 1% CSE for 24 h, followed by EV isolation using ultrafiltration size exclusion chromatography. Isolated labelled with tandem mass tags...
The human gastrointestinal tract harbors a diverse and complex microbiome, which interacts in variety of ways with the host. There is compelling evidence that gut microbial dysbiosis, defined as an alteration diversity abundance intestinal microbes, etiological factor inflammatory bowel disease (IBD). Membrane vesicles (MVs), are nano-sized particles released by bacteria, have been found to interact host modulate development function immune system. As result MVs suggested play critical role...