A5076076501- Platelet Disorders and Treatments
- Venous Thromboembolism Diagnosis and Management
- Antiplatelet Therapy and Cardiovascular Diseases
- Atrial Fibrillation Management and Outcomes
- Blood properties and coagulation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Blood Coagulation and Thrombosis Mechanisms
- Oral microbiology and periodontitis research
- Blood transfusion and management
- COVID-19 Clinical Research Studies
- Salivary Gland Disorders and Functions
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hemophilia Treatment and Research
- Acute Myocardial Infarction Research
- Blood groups and transfusion
- Blood donation and transfusion practices
- Muscle and Compartmental Disorders
- Periodontal Regeneration and Treatments
- Heparin-Induced Thrombocytopenia and Thrombosis
- Oral and gingival health research
- Maternal and fetal healthcare
- Clinical Laboratory Practices and Quality Control
- Genomics and Rare Diseases
- Blood disorders and treatments
- Inflammatory Biomarkers in Disease Prognosis
Maastricht University
2016-2025
Maastricht University Medical Centre
2016-2025
Radboud University Nijmegen
2019-2024
Radboud University Medical Center
2019-2024
Sanquin
2024
GGz centraal
2021
Radboud Institute for Molecular Life Sciences
2020
DNA Diagnostic (Denmark)
2017
Cardiff University
2016
University Medical Center
2014-2016
Mean platelet volume (MPV) is associated with various diseases. Several authors reported anticoagulant and time dependency. Therefore, standardized laboratory methods are essential. The aim of this study was to standardize the MPV measurement. Blood collected in potassium-ethylenediaminetetra-acid (EDTA) sodium-citrate tubes. First, count were determined every half hour for 4 hours 20 healthy volunteers. same parameters acquired from a second group 100 donors. We measured at point highest...
Excessive bleeding at surgery is a feared complication in patients with inherited platelet disorders. However, very few studies have evaluated the frequency of surgical these hemorrhagic We performed worldwide, multicentric, retrospective study to assess complications surgery, preventive and therapeutic approaches adopted, their efficacy disorders: Surgery Platelet disorders And Therapeutic Approach (SPATA) study. rated outcome 829 procedures carried out 423 well-defined forms 238 function...
ABSTRACT Extracellular vesicles (EVs) are mediators of cell communication during health and disease, abundantly released by platelets upon activation or ageing. Platelet EVs exert modulatory effects on immune vascular cells. may modulate the function smooth muscle cells (SMC). were isolated from platelet‐rich plasma incubated with SMC in order to assess binding, proliferation, migration pro‐inflammatory phenotype firmly bound resting through platelet integrin α IIb β 3 , while binding also...
Thus far, validated whole blood assays used in vitro fibrinolysis experiments using thromboelastometry (ROTEM) are lacking or have yet to be tested humans. The objective was first, establish a standardized modified ROTEM approach detect both hypo- and hyperfibrinolysis. And second, perform technical clinical validation of the assay.Blood healthy volunteers, patients with sepsis, after cardiothoracic surgery, pregnant women, cirrhotic liver disease patients. A tissue factor (TF) activated...
Abstract Thrombo-inflammation describes the complex interplay between blood coagulation and inflammation that plays a critical role in cardiovascular diseases. The third Maastricht Consensus Conference on Thrombosis assembled basic, translational, clinical scientists to discuss origin potential consequences of thrombo-inflammation etiology, diagnostics, management patients with disease, including myocardial infarction, stroke, peripheral artery disease. This article presents state-of-the-art...
Salivary protein, albumin and cystatin concentrations were investigated in subjects with a healthy periodontium patients gingivitis or periodontitis. Protein saliva of individuals periodontitis significantly increased compared subjects. protein appeared to be positively correlated all the groups, which suggests that increase salivary concentration is caused by leakage plasma proteins. Cystatin when group (p < 0.01). In group, was only weakly concentrations, activity found derived from...
Cystatins are physiological inhibitors of cysteine proteinases and they widely distributed in human tissues body fluids including saliva. We previously reported an increased cystatin activity whole saliva gingivitis periodontitis subjects. Based on this result we decided to investigate the type origin cystatins involved by collecting both parotid 25 healthy 30 Saliva samples were quantified for S C enzyme-linked immunosorbent assay activities measured toward papain. Besides, three other...
Objective— Platelet- and fibrin-dependent thrombus formation is regulated by blood flow exposure of collagen tissue factor. However, interactions between these blood-borne vascular components are not well understood. Approach Results— Here, we developed a method to assess whole-blood on microspots with defined amounts factor, allowing determination the mechanical properties intrathrombus composition. Confining content resulted in diminished platelet deposition fibrin at high shear...
Light transmission aggregation (LTA) is the gold standard for diagnosis of platelet function disorders (PFDs), but it time-consuming and limited to specialized laboratories. Whole-blood impedance aggregometry (Multiplate) analyzer (PFA) may be used as rapid screening tools exclude PFDs. The aim this study assess diagnostic performance Multiplate PFA PFDs, detected by LTA.Data from preoperative patients, patients referred hematologist bleeding evaluation, with a diagnosed disorder were used....
Background: Coronavirus Disease 2019 (COVID-19) patients often present with thromboembolic events. In COVID-19 patients, routine hemostatic assays cannot correctly identify at risk for Viscoelastic testing rotational thromboelastometry (ROTEM) might improve the characterization of COVID-19-associated coagulopathy. Objective: To unravel underlying coagulopathy and fibrinolysis over time as measured by serial assessment heparin-independent (FIBTEM EXTEM) illustrating (tissue plasminogen...
Cystatins are inhibitors of cysteine proteinases and could play a protective regulatory role under inflammatory conditions. Since total cystatin activity whole saliva was increased in periodontal patients (Henskens et al., 1993), we wanted to investigate the types or origins cystatins involved this increase. Distinct were identified by isoelectric focusing immunoblotting with specific antibodies against one salivary acidic isoforms, S. widely distributed basic C. Clarified human (CHWS)...
Coated platelets, formed by collagen and thrombin activation, have been characterized in different ways: i) the formation of a protein coat α-granular proteins; ii) exposure procoagulant phosphatidylserine; or iii) high fibrinogen binding. Yet, their functional role has remained unclear. Here we used novel transglutaminase probe, Rhod-A14, to identify subpopulation platelets with cross-linked coat, compared this other platelet subpopulations using panel assays. Platelet stimulation...
ObjectivesRotational thromboelastometry (ROTEM)-guided transfusion algorithms in cardiac surgery have been proven to be successful reducing blood loss randomized controlled trials. Using an institutional hemostasis registry of patients (HEROES-CS), the authors hypothesized that use ROTEM-guided would save products and overall costs every day practice.DesignObservational, prospective open cohort database.SettingSingle-center academic hospital.ParticipantsCardiac...
Traditional coagulation tests are included in emergency guidelines for management of patients on direct oral anticoagulants (DOACs) who experience acute bleeding or require surgery. We determined the ability traditional and fast whole blood thromboelastography (ROTEM®) to screen anticoagulation activity dabigatran rivaroxaban as low 30 ng/mL. One hundred eighty-four citrated samples (75 dabigatran, 109 rivaroxaban) were collected from with non-valvular atrial fibrillation (NVAF), perform...