A5080737897- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Blood properties and coagulation
- Blood groups and transfusion
- Extracellular vesicles in disease
- Blood Coagulation and Thrombosis Mechanisms
- Inflammatory Biomarkers in Disease Prognosis
- Tendon Structure and Treatment
- Periodontal Regeneration and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Shoulder Injury and Treatment
- Mental Health and Psychiatry
- Cell Adhesion Molecules Research
- Blood transfusion and management
- Hemoglobinopathies and Related Disorders
- Erythrocyte Function and Pathophysiology
- Complement system in diseases
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Heparin-Induced Thrombocytopenia and Thrombosis
- Sepsis Diagnosis and Treatment
- Trauma and Emergency Care Studies
- Acute Myocardial Infarction Research
- Hemophilia Treatment and Research
- Prion Diseases and Protein Misfolding
University of Birmingham
2015-2024
Queen Elizabeth Hospital Birmingham
2016-2024
NIHR Surgical Reconstruction and Microbiology Research Centre
2017-2024
Institute of Art
2015-2022
University Hospitals Birmingham NHS Foundation Trust
2018-2021
Consejo Nacional de Investigaciones Científicas y Técnicas
2021
University of Buenos Aires
2021
Institute for Medical Research
2021
University of Liverpool
2019
Council of Science Editors
2015-2018
Cellular microvesicles and nanovesicles (exosomes) are involved in many disease processes have major potential as biomarkers. However, developments this area constrained by limitations the technology available for their measurement. Here we report on use of fluorescence nanoparticle tracking analysis (NTA) to rapidly size phenotype cellular vesicles. In system vesicles visualized light scattering using a microscope. A video is taken, NTA software tracks brownian motion individual calculates...
Introduction Nonoperative management is presently considered the treatment modality of choice in over 50% adult patients sustaining blunt hepatic trauma who meet inclusion criteria. A multicenter study was retrospectively undertaken to assess whether combined experiences at level I centers could validate currently reported high success rate, low morbidity, and virtually nonexistent mortality associated with this approach. Thirteen accrued 404 injuries managed nonoperatively last 5 years....
Summary The guideline writing group was selected to be representative of UK‐based medical experts. MEDLINE systematically searched for publications in English up the Summer 2010 using key words platelet, platelet function testing and aggregometry. Relevant references generated from initial papers published guidelines/reviews were also examined. Meeting abstracts not included. produced draft guideline, which subsequently revised agreed by consensus. Further comment made members Haemostasis...
The aim of this study was to measure neutrophil function longitudinally following burn injury and examine the relationship between dysfunction sepsis.Sepsis prevalence its associated mortality is high injury, sepsis diagnosis complicated by ongoing inflammatory response. Previous studies have suggested that may underlie infection rates postburn; however, has not been thoroughly characterized over time in burns patients.Neutrophil phagocytosis, oxidative burst capacity, extracellular trap...
Essentials Platelet extracellular vesicles (EVs) concentrations measured by flow cytometers are incomparable. A model is applied to convert ambiguous scatter units EV diameter in nanometer. Most included lack the sensitivity detect EVs of 600 nm and smaller. The outperforms polystyrene beads for comparability platelet concentrations.Background Detection cytometry has poor interlaboratory comparability, owing differences cytometer (FCM) sensitivity. Previous workshops distributed set a...
It is possible that platelet activation may play a pathogenic role in the increased risk of thrombosis associated with antiphospholipid antibodies (APA). In this study, levels vivo were measured 20 patients primary syndrome (PAPS) and 30 systemic lupus erythematosus (SLE) (14 whom had secondary APS) using sensitive flow cytometry. Soluble P-selectin also assayed. Platelet CD63 expression was significantly higher PAPS than normal controls (P = 0.007), as well SLE without APS 0.03 P 0.002...
The origin of platelet alpha-granule fibrinogen (Fg), whether from endogeneous synthesis or exogeneous derivation, remains unknown. Although Fg biosynthesis by megakaryocytes (MK) has been suggested, recent studies have demonstrated that certain alpha-granular proteins originate primarily plasma. To study the Fg, platelet-associated was measured ELISA and Western blotting, localized immunofluorescence immunoelectron microscopy in a patient with symptomatic congenital afibrinogenemia before...
We have previously shown that fresh-frozen plasma (FFP) contains red blood cell-derived procoagulant microparticles (MPs) are removable by 0.2 microm filtration. Given the limitations of current methods for accurately sizing MPs, we applied novel approach dynamic light scattering (DLS) to characterize size distributions these MPs within FFP.Fresh-frozen was prepared from Group A and O donations (n = 10 each) after an overnight hold whole at 4 degrees C. On day analysis, thawed 37 C daughter...