A5005380598- Calcium Carbonate Crystallization and Inhibition
- Bone Tissue Engineering Materials
- Paleontology and Stratigraphy of Fossils
- RNA modifications and cancer
- Photovoltaic Systems and Sustainability
- Integrated Energy Systems Optimization
- RNA and protein synthesis mechanisms
- Cellular Mechanics and Interactions
- Renewable energy and sustainable power systems
- Cephalopods and Marine Biology
- Photovoltaic System Optimization Techniques
- Skin and Cellular Biology Research
- Silk-based biomaterials and applications
- Smart Grid Energy Management
- Electric Power System Optimization
- Marine Bivalve and Aquaculture Studies
- RNA Research and Splicing
- Polymer Surface Interaction Studies
- Energy Efficiency and Management
- Diatoms and Algae Research
- Global Energy and Sustainability Research
- Hemoglobinopathies and Related Disorders
- Global Energy Security and Policy
- Iron Metabolism and Disorders
- Renewable Energy and Sustainability
University of Stuttgart
2016-2024
Administration for Community Living
2024
Focus (Germany)
2019-2022
WIP - Renewable Energies
1995-2019
European Committee for Standardization
2018
Leibniz-Institute for New Materials
2009-2017
University Research Co (United States)
2016
Leibniz Association
2009-2014
University of Regensburg
2005-2013
Boston University
2013
Abstract The larval shells of the marine bivalves Mercenaria mercenaria and Crassostrea gigas are investigated by polarized light microscopy, infrared spectroscopy, Raman imaging scanning electron microscopy. Both species contain similar shell ultrastructures. We show that amorphous calcium carbonate (ACC), in addition to aragonite. aragonite is much less crystalline than nonbiogenic further initially deposited mineral phase predominantly ACC subsequently partially transforms into postset...
The pathways of thermal instability amino acids have been unknown. New mass spectrometric data allow unequivocal quantitative identification the decomposition products.Calorimetry, thermogravimetry and spectrometry were used to follow eight G, C, D, N, E, Q, R H between 185 °C 280 °C. Endothermic heats 72 151 kJ/mol are needed form 12 70% volatile products. This process is neither melting nor sublimation. With exception cysteine they emit mainly H2O, some NH3 no CO2. Cysteine produces CO2...
Essentials Platelet extracellular vesicles (EVs) concentrations measured by flow cytometers are incomparable. A model is applied to convert ambiguous scatter units EV diameter in nanometer. Most included lack the sensitivity detect EVs of 600 nm and smaller. The outperforms polystyrene beads for comparability platelet concentrations.Background Detection cytometry has poor interlaboratory comparability, owing differences cytometer (FCM) sensitivity. Previous workshops distributed set a...
AbstractThe highly stable nature of globin mRNA is central importance to erythroid cell differentiation. We have previously identified cytidine-rich (C-rich) segments in the human α-globin 3′ untranslated region (α-3′UTR) which are critical maintenance stability transfected cells. In present studies, we detected trans-acting factors interact with these cis elements mediate this stabilizing function. A sequence-specific ribonucleoprotein (RNP) complex assembled after incubation α-3′UTR a...
Perlucin isolated from abalone nacre consists of 155 amino acids including a glycosylated asparagine. The sequence the first 130 shows high similarity to C‐type carbohydrate‐recognition domains asialoglycoprotein receptors and other members group lectins but also weaker related proteins without carbohydrate‐binding activity. This module is followed by short C‐terminal domain containing two almost identical repeats with length 10 acids. Solid phase assays show divalent metal ion‐dependent...
Very little is known about the mechanisms mediating longevities of mRNAs. As a means identifying potential cis- and trans-acting elements which stabilize an individual mRNA, naturally occurring mutations that decreased stability normally long-lived globin mRNA were analyzed. Our previous studies demonstrated subset allowed translating ribosome to read through into alpha 2-globin 3' nontranslated region (NTR) targeted mutant mRNAs for accelerated turnover in erythroid cells but not several...
Although globin mRNAs are considered prototypes of highly stable messages, the mechanisms responsible for their longevity remain largely undefined. As an initial step in identifying potential cis-acting elements or structures which contribute to stability, we analyzed defect expression a naturally occurring alpha 2-globin mutant, Constant Spring (CS). The CS mutation is single-base change translation termination codon (UAA-->CAA) that allows ribosome read through into 3' nontranslated...
Chitin is a key component in mollusk nacre formation. However, the enzyme complex responsible for chitin deposition shell remained unknown. We cloned and characterized synthase of marine bivalve Atrina rigida . present here first sequence from invertebrates containing an unconventional myosin motor head domain. further show that homologous gene expressed forming tissue larval Mytilus galloprovincialis even early embryonic stages. The new data presented are clear‐cut indication functional...
Chitin self-assembly provides a dynamic extracellular biomineralization interface. The insoluble matrix of larval shells the marine bivalve mollusc Mytilus galloprovincialis consists chitinous material that is distributed and structured in relation to characteristic shell features. Mollusc chitin synthesized via complex transmembrane synthase with an intracellular myosin motor domain.Enzymatic synthesis was investigated vivo by using small-molecule drug NikkomycinZ, structural analogue sugar...
This paper reviews the present status and outlook of building integrated photovoltaics (BIPV) market on a global European scale. In particular, it provides comprehensive review situation future trends for Austria, Cyprus, France, Germany, Italy Netherlands until year 2020. addition, as education is seen one barriers BIPV deployment, results survey are presented that was conducted among stakeholders with aim to identify major knowledge gaps in target audiences well teaching goals. From...
High-resolution synchrotron X-ray powder diffraction (XRD) combined with the Rietveld refinement method and confocal laser scanning microscopy (CLSM) were utilized in this study to elucidate interaction between a recombinant biomineralization protein (perlucin) fused green fluorescent (GFP) synthetic calcite. Although perlucin is insoluble, its solubility was increased via fusion highly soluble GFP. We demonstrate that GFP-perlucin derivatives become incorporated into calcite structure...
Synthesis of normal human hemoglobin A, alpha 2 beta 2, is based upon balanced expression genes in the alpha-globin gene cluster on chromosome 16 and beta-globin 11. Full levels erythroid-specific activation depend sequences located at a considerable distance 5' to gene, referred as locus-activating or dominant control region. The existence an analogous element(s) upstream has been suggested from observations naturally occurring deletions experimental studies. We have identified individual...
Abstract The feathers in the train of peacock serve not for flying but sexual display. They are long, slender beams loaded bending by their own weight. An outer circular conical shell, cortex, is filled a closed foam 7.6% relative density, medulla, both feather keratin. Outer diameter and thickness cortex decrease linearly from body toward tip. This self‐similar geometry leads to division labor. (longitudinal Young's modulus 3.3 GPa, transverse 1 GPa) provides 96% longitudinal strength...
We collected test reactions which can be applied in an easy and reproducible way to the chemical composition of abalone organic matrix. Several biochemical were interlamellar matrix nacre study content nature polysaccharides. The preparation polysaccharide was examined parallel by light microscopy. core is covered a honeycomb-like structure, completely removed protease subtilisin under release hydrophobic amino acid proline. honeycomb pattern its size shape exactly inverse aragonite tablets,...
Abstract The size, morphology and species-specific texture of mollusc shell biominerals is one the unresolved questions in nature. In search molecular control principles, chitin has been identified by Weiner Traub (FEBS Lett. 1980, 111:311–316) as organic compounds with a defined co-organization mineral phases. Chitin fibers can be aligned certain mineralogical axes crystalline calcium carbonate manner. These original observations motivated functional characterization forming enzymes...
Although globin mRNAs are considered prototypes of highly stable messages, the mechanisms responsible for their longevity remain largely undefined. As an initial step in identifying potential cis-acting elements or structures which contribute to stability, we analyzed defect expression a naturally occurring alpha 2-globin mutant, Constant Spring (CS). The CS mutation is single-base change translation termination codon (UAA-->CAA) that allows ribosome read through into 3' nontranslated region...
Chondrocytes grown in suspension contain both type I and II collagen mRNAs, yet synthesize only collagen. The inability of chondrocytes to the alpha 2 subunit collagen, 2(I), results from a severely reduced translation elongation rate (Bennett, V.D., Adams, S.L. (1987) J. Biol. Chem. 262, 14806-14814). Furthermore, 2(I) mRNAs are translated inefficiently vitro appear slightly smaller than those other cells (Focht, R.J., (1984) Mol. Cell. 4, 1843-1852). These observations suggest that may be...