A5079465551- Venous Thromboembolism Diagnosis and Management
- Lymphatic System and Diseases
- Inflammasome and immune disorders
- COVID-19 Clinical Research Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Platelet Disorders and Treatments
- Immune Cell Function and Interaction
- Erythrocyte Function and Pathophysiology
- Renal Diseases and Glomerulopathies
- Complement system in diseases
- Asthma and respiratory diseases
- Nanoplatforms for cancer theranostics
- Heme Oxygenase-1 and Carbon Monoxide
- IL-33, ST2, and ILC Pathways
- Biomarkers in Disease Mechanisms
- Photoacoustic and Ultrasonic Imaging
- Acute Ischemic Stroke Management
- Mast cells and histamine
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Lymphatic Disorders and Treatments
- Coronary Interventions and Diagnostics
- melanin and skin pigmentation
- Thermal Regulation in Medicine
- Eosinophilic Disorders and Syndromes
- S100 Proteins and Annexins
University of Birmingham
2019-2024
Monash Medical Centre
2023-2024
Monash Health
2023-2024
Monash University
2023-2024
Abstract Thrombo-inflammation describes the complex interplay between blood coagulation and inflammation that plays a critical role in cardiovascular diseases. The third Maastricht Consensus Conference on Thrombosis assembled basic, translational, clinical scientists to discuss origin potential consequences of thrombo-inflammation etiology, diagnostics, management patients with disease, including myocardial infarction, stroke, peripheral artery disease. This article presents state-of-the-art...
Abstract S100A8/A9, also known as “calprotectin” or “MRP8/14,” is an alarmin primarily secreted by activated myeloid cells with antimicrobial, proinflammatory, and prothrombotic properties. Increased plasma levels of S100A8/A9 in thrombo-inflammatory diseases are associated thrombotic complications. We assessed the presence lung autopsies from patients COVID-19 investigated molecular mechanism which affects platelet function thrombosis. were increased sustained high during hospitalization...
Thrombotic complications and vasculopathy have been extensively associated with severe COVID-19 infection; however, the mechanisms inducing endotheliitis disruption of endothelial integrity in microcirculation are poorly understood. We hypothesized that within vessel wall, pericytes preferentially take up viral particles mediate subsequent loss vascular integrity.
Background The systemic inflammatory response post-SARS-CoV-2 infection increases pro-inflammatory cytokine production, multi-organ damage, and mortality rates. Mast cells (MC) modulate thrombo-inflammatory disease progression ( e.g. , deep vein thrombosis) the post-infection. Objective To enhance our understanding of contribution MC their proteases in SARS-CoV-2 pathogenesis disease, which might help to identify novel therapeutic targets. Methods chymase (CMA1), carboxypeptidase A3 (CPA3),...
C-type lectin-like receptor 2 (CLEC-2) is highly expressed on platelets and a subpopulation of myeloid cells, critical in lymphatic development. CLEC-2 has been shown to support thrombus formation at sites inflammation, but have minor/negligible role hemostasis. This identifies as promising therapeutic target thromboinflammatory disorders, without hemostatic detriment. We utilized GPIbα-Cre recombinase mouse for more restricted deletion platelet-CLEC-2 than the previously used PF4-Cre mouse....
Platelets play a key role in the development, progression and resolution of inflammatory response during sterile inflammation infection, although mechanism is not well understood. Here we show that platelet CLEC-2 reduces tissue by regulating macrophage activation trafficking from inflamed tissues. The immune regulatory function depends on expression its ligand, podoplanin, upregulated macrophages independent secretion. Mechanistically, also recombinant CLEC-2-Fc accelerates actin...
The NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome has been described in both immune cells platelets, but its role the megakaryocyte (MK) lineage remains elusive. aim of this study was to explore NLRP3 megakaryocytes platelets. We generated Nlrp3 A350V/+/Gp1ba-CreKI/+ mice carrying a mutation genetically similar one observed human Muckle-Wells syndrome, which leads hyperactivity specifically MK Platelets from mutant expressed elevated levels precursor active form...
Free labile hemin acts as a damage-associated molecular pattern during acute and chronic hemolysis muscle injury, supporting platelet activation thrombosis.
Tissue microenvironments during physiology and pathology are highly complex, meaning dynamic cellular activities their interactions cannot be accurately modelled
Abstract Objective Thrombotic complications and vasculopathy have been extensively associated with severe COVID-19 infection, however the mechanisms by which endotheliitis is induced remain poorly understood. Here we investigate vascular permeability in context of SARS-CoV-2-mediated patient samples a organoid model. Methods Results We report presence Spike glycoprotein pericytes pericyte activation increased endothelial post-mortem lung autopsies. A pronounced decrease expression adhesion...
Abstract Macrophage recruitment during sterile inflammation and infection is essential to clear pathogens, apoptotic cells debris. However, persistent macrophage accumulation leads chronic inflammation. Platelets are emerging as key modulators of the inflammatory response. Here, we identify that platelet C-type-lectin-like receptor-2 (CLEC-2) a crucial immunomodulatory receptor through interaction with podoplanin, upregulated on macrophages. Mechanistically, CLEC-2 upregulates expression...
Abstract The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome has been described in both immune cells platelets but its role the megakaryocyte (MK) lineage remains elusive. Here, we generated Nlrp3 A350V/+ / Gp1ba -Cre KI/+ mice carrying MK/platelet-specific mutation associated with human Muckle-Wells syndrome. Platelets from these expressed elevated levels of precursor active form caspase-1 suggesting hyperactivity NLRP3 inflammasome. developed normally had normal...