A5088356352- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Monoclonal and Polyclonal Antibodies Research
- Liver physiology and pathology
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- T-cell and B-cell Immunology
- Liver Diseases and Immunity
- Carbohydrate Chemistry and Synthesis
- Phagocytosis and Immune Regulation
- Glycosylation and Glycoproteins Research
- Receptor Mechanisms and Signaling
- Immunodeficiency and Autoimmune Disorders
- Bacteriophages and microbial interactions
- Liver Disease and Transplantation
- Galectins and Cancer Biology
- Infection Control and Ventilation
- Pancreatic function and diabetes
- Antimicrobial agents and applications
- Macrophage Migration Inhibitory Factor
- SARS-CoV-2 detection and testing
University of Birmingham
2016-2025
Institute of Immunology
2023
National Health Service
2021
Birmingham City University
2020
Albany Molecular Research (United States)
2020
Shanghai Genon Biological Products (China)
2020
Cancer Research UK Clinical Trials Unit
2020
NIHR Birmingham Biomedical Research Centre
2016-2020
University Hospitals Birmingham NHS Foundation Trust
2019-2020
NIHR Birmingham Liver Biomedical Research Unit
2011-2018
Hepatitis C virus (HCV) infection of Huh-7.5 hepatoma cells results in focal areas where transmission is potentiated by cell-cell contact. To define route(s) transmission, HCV was allowed to infect the presence or absence antibodies that neutralize cell-free infectivity. Neutralizing (nAbs) reduced infectivity >95% and had minimal effect(s) on frequency infected culture. assess whether transfer viral occurs, HCV-infected were cocultured with fluorescently labeled naïve nAbs. Enumeration...
Liver fibrosis is a wound healing response to chronic liver injury and inflammation in which macrophages infiltrating monocytes participate both the development resolution phase. In humans, three monocyte subsets have been identified: classical CD14++CD16-, intermediate CD14++CD16+, nonclassical CD14+CD16++ monocytes. We studied phenotype function of these peripheral blood tissue from patients with inflammatory fibrotic diseases. The frequency intrahepatic increased disease compared control...
Acute liver failure (ALF) is characterised by overwhelming hepatocyte death and inflammation with massive infiltration of myeloid cells in necrotic areas. The mechanisms underlying resolution acute hepatic are largely unknown. Here, we aimed to investigate the impact Mer tyrosine kinase (MerTK) during ALF also examine how microenvironmental mediator, secretory leucocyte protease inhibitor (SLPI), governs this response.Flow cytometry, immunohistochemistry, confocal imaging gene expression...
The virological and cellular consequences of persistent hepatitis C virus (HCV) infection have been elusive due to the absence requisite experimental systems. Here, we report establishment characteristics in vitro human hepatoma-derived cells by a recently described HCV genotype 2a infectious molecular clone. Persistent was characterized selection viral variants that displayed accelerated expansion kinetics, higher peak titers, increased buoyant densities. Sequencing analysis revealed single...
Abstract The common lymphatic endothelial and vascular receptor (CLEVER-1; also known as FEEL-1 stabilin-1) is a recycling intracellular trafficking with multifunctional properties. In this study, we demonstrate increased expression of CLEVER-1/stabilin-1 at sites leukocyte recruitment to the inflamed human liver including sinusoids, septal vessels, lymphoid follicles in inflammatory disease tumor-associated vessels hepatocellular carcinoma. We used primary cultures hepatic sinusoidal cells...
Hepatitis C virus (HCV) enters cells via a pH- and clathrin-dependent endocytic pathway. Scavenger receptor BI (SR-BI) CD81 are important entry factors for HCV internalization into target cells. The SR-BI gene gives rise to at least two mRNA splice variants, SR-BII, which differ in their termini. remains poorly understood, but SR-BII is reported endocytose pathway, making it an attractive internalization. We demonstrate that soluble E2 can interact with human SR-BII. Increased expression of...
Abstract The glucagon-like peptide-1 receptor (GLP1R) is a class B G protein-coupled (GPCR) involved in metabolism. Presently, its visualization limited to genetic manipulation, antibody detection or the use of probes that stimulate activation. Herein, we present LUXendin645 , far-red fluorescent GLP1R antagonistic peptide label. produces intense and specific membrane labeling throughout live fixed tissue. signaling can additionally be evoked when allosterically modulated presence . Using...
Airborne pathogens pose high risks in terms of both contraction and transmission within the respiratory pathways, particularly nasal region. However, there is little way adequate intervention that can protect an individual or prevent further spread. This study reports on a formulation with capacity to combat such challenges, focusing severe acute syndrome coronavirus-2 (SARS-CoV-2). Formulation polysaccharide-based spray, known for its mucoadhesive properties, undertaken it characterized...
Vaccination prevents severe morbidity and mortality from COVID-19 in the general population. The immunogenicity efficacy of SARS-CoV-2 vaccines patients with antibody deficiency is poorly understood.
Superinfection exclusion is the ability of an established virus infection to interfere with by a second virus. In this study, we found that Huh-7.5 cells acutely infected hepatitis C (HCV) genotype 2a (chimeric strain J6/JFH) and harboring HCV 1a, 1b, or full-length subgenomic replicons were resistant cell culture-produced (HCVcc). Replicon-containing became permissive for HCVcc after treatment HCV-specific protease inhibitor. With exception J6/JFH-FLneo replicon, replicon-containing...
Abstract The COVID-19 pandemic has demonstrated the real need for mechanisms to control spread of airborne respiratory pathogens. Thus, preventing disease from pathogens come forefront public consciousness. This brought an increasing demand novel technologies prioritise clean air. In this study we report on efficacy biocide treated filters and their antimicrobial activity against bacteria, fungi viruses. reported here are shown kill pathogens, such as Candida albicans , Escherichia coli MRSA...
Thrombotic complications and vasculopathy have been extensively associated with severe COVID-19 infection; however, the mechanisms inducing endotheliitis disruption of endothelial integrity in microcirculation are poorly understood. We hypothesized that within vessel wall, pericytes preferentially take up viral particles mediate subsequent loss vascular integrity.
Hepatitis C virus (HCV) causes progressive liver disease and is a major risk factor for the development of hepatocellular carcinoma (HCC). However, role infection in HCC pathogenesis poorly understood. We investigated effect(s) HCV viral glycoprotein expression on hepatoma biology to gain insights into associated HCC.We assessed polarity, migration invasion.HCV glycoproteins perturb tight adherens junction protein expression, increase epithelial mesenchymal transition markers Snail Twist via...
CD4+ T cells play critical roles in directing immunity, both as helper and regulatory (Treg) cells. Here, we demonstrate that hepatocytes can modulate cell populations through engulfment of live lymphocytes. We term this phenomenon enclysis to reflect the specific enclosure hepatocytes. Enclysis is selective for but not CD8+ cells, independent antigen-specific activation, occurs human vitro, ex vivo, vivo. Intercellular adhesion molecule 1 (ICAM-1) facilitates early internalization, whereas...
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic has caused a significant number of fatalities and worldwide disruption. To identify drugs to repurpose treat SARS-CoV-2 infections, we established screen measure the dimerization angiotensin-converting enzyme 2 (ACE2), primary receptor for virus. This identified fenofibric acid, active metabolite fenofibrate. Fenofibric acid also destabilized receptor-binding domain (RBD) viral spike protein inhibited RBD binding ACE2...