A5013838935- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood properties and coagulation
- Blood Coagulation and Thrombosis Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Blood groups and transfusion
- Diabetes Treatment and Management
- Heparin-Induced Thrombocytopenia and Thrombosis
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell Adhesion Molecules Research
- Dialysis and Renal Disease Management
- Peripheral Artery Disease Management
- Biomarkers in Disease Mechanisms
- Vitamin K Research Studies
- Circular RNAs in diseases
- Apelin-related biomedical research
- Acute Myocardial Infarction Research
- Cholesterol and Lipid Metabolism
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Chronic Myeloid Leukemia Treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Liver physiology and pathology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Inflammatory Biomarkers in Disease Prognosis
Maastricht University
2014-2024
RWTH Aachen University
2018-2024
Universitätsklinikum Aachen
2018-2023
Maastricht University Medical Centre
2015-2021
Cardiff University
2016
University Medical Center
2016
Abstract Assays measuring platelet aggregation (thrombus formation) at arterial shear rate mostly use collagen as only platelet-adhesive surface. Here we report a multi-surface and multi-parameter flow assay to characterize thrombus formation in whole blood from healthy subjects patients with function deficiencies. A systematic comparison is made of 52 adhesive surfaces components activating the main receptors, eight output parameters reflecting distinct stages formation. Three types can be...
Novel platelet and megakaryocyte transcriptome analysis allows prediction of the full or theoretical proteome a representative human platelet. Here, we integrated established proteomes from six cohorts healthy subjects, encompassing 5.2 k proteins, with two novel genome-wide transcriptomes (57.8 mRNAs). For 14.8 protein-coding transcripts, assigned proteins to 21 UniProt-based classes, based on their preferential intracellular localization presumed function. This classified...
Abstract The leading cause of heart disease in developed countries is coronary atherosclerosis, which not simply a result ageing but chronic inflammatory process that can lead to acute clinical events upon atherosclerotic plaque rupture or erosion and arterial thrombus formation. composition location plaques determine the phenotype lesion whether it more likely erode. Although both initiate platelet activation on exposed vascular surface, contribution platelets formation differs between two...
In combination with microspotting, whole-blood microfluidics can provide high-throughput information on multiple platelet functions in thrombus formation. Based assessment of the inter- and intra-subject variability parameters microspot-based formation, we aimed to determine factors contributing this variation. Blood samples from 94 genotyped healthy subjects were analyzed for conventional phenotyping: i.e. hematologic parameters, glycoprotein (GP) expression levels activation markers (24...
Objective— Platelet- and fibrin-dependent thrombus formation is regulated by blood flow exposure of collagen tissue factor. However, interactions between these blood-borne vascular components are not well understood. Approach Results— Here, we developed a method to assess whole-blood on microspots with defined amounts factor, allowing determination the mechanical properties intrathrombus composition. Confining content resulted in diminished platelet deposition fibrin at high shear...
Coated platelets, formed by collagen and thrombin activation, have been characterized in different ways: i) the formation of a protein coat α-granular proteins; ii) exposure procoagulant phosphatidylserine; or iii) high fibrinogen binding. Yet, their functional role has remained unclear. Here we used novel transglutaminase probe, Rhod-A14, to identify subpopulation platelets with cross-linked coat, compared this other platelet subpopulations using panel assays. Platelet stimulation...
Scott syndrome is a rare bleeding disorder, characterized by altered Ca2+-dependent platelet signaling with defective phosphatidylserine (PS) exposure and microparticle formation, linked to mutations in the ANO6 gene, encoding anoctamin (Ano)6. We investigated how complex phenotype of this expression Anos or other ion channels. Mice were generated heterozygous homozygous deficiency Ano6, Ano1, KCa3.1 Gardos channel. Platelets from these mice extensively analyzed on molecular functions...
Abstract Atherothrombosis is a leading cause of cardiovascular mortality and long-term morbidity. Platelets coagulation proteases, interacting with circulating cells in different vascular beds, modify several complex pathologies including atherosclerosis. In the second Maastricht Consensus Conference on Thrombosis, this theme was addressed by diverse scientists from bench to bedside. All presentations were discussed audience members results these discussions incorporated final document that...
BackgroundEspecially in disease conditions, platelets can encounter activating agents circulation.ObjectivesTo investigate the extent to which previously activated be reactivated and whether in-and reactivation applies different aspects of platelet activation thrombus formation.MethodsShort-and long-term effects glycoprotein VI (GPVI) G protein-coupled receptor (GPCR) stimulation on aggregation potential were compared via flow cytometry plate-based aggregation. Using fluorescence electron...
Severe thrombocytopenia (≤50×109 platelets/L) due to hematological malignancy and intensive chemotherapy is associated with an increased risk of clinically significant bleeding. Since the bleeding not linked platelet count only, other hemostatic factors must be involved. We studied function in 77 patients acute leukemia, multiple myeloma or malignant lymphoma, who experienced chemotherapy-induced thrombocytopenia. Platelets from all - independent disease treatment type were a variable extent...
The Fourth Maastricht Consensus Conference on Thrombosis included the following themes. Theme 1: "coagulome" as a critical driver of cardiovascular disease. Blood coagulation proteins also play divergent roles in biology and pathophysiology, related to specific organs, including brain, heart, bone marrow, kidney. Four investigators shared their views these organ-specific topics. 2: Novel mechanisms thrombosis. Mechanisms linking factor XII fibrin, structural physical properties, contribute...
Summary Blood dilution after transfusion fluids leads to diminished coagulant activity monitored by rotational thromboelastometry, assessing elastic fibrin clot formation, or thrombin generation testing. We aimed determine the contributions of blood cells (platelets, red cells) and plasma factors (fibrinogen, prothrombin complex concentrate) formation under conditions haemodilution in vitro vivo. Whole diluted was supplemented with platelets, cells, fibrinogen concentrate (PCC)....
In patients with diabetes mellitus, increased platelet reactivity predicts cardiac events. Limited evidence suggests that DPP-4 (dipeptidyl peptidase 4) influences platelets via GLP-1 (glucagon-like peptide 1)-dependent effects. Because inhibitors are frequently used in mellitus to improve the GLP-1-regulated glucose metabolism, we characterized role of inhibition and native intact versus DPP-4-cleaved on flow-dependent thrombus formation mouse human blood. Approach Results: An ex vivo whole...
Platelets from healthy donors display heterogeneity in responsiveness to agonists. The response thresholds of platelets are controlled by multiple bioactive molecules, acting as negatively or positively priming substances. Higher circulating levels substances adenosine and succinate, well the occurrence hypercoagulability, have been described for patients with ischaemic heart disease. Here, we present an improved methodology flow cytometric analyses platelet activation characterisation...
In patients with acute coronary syndrome, dual antiplatelet therapy aspirin and a P2Y12 inhibitor like prasugrel is prescribed for one year. Here, we investigated how the hemostatic function of platelets recovers after discontinuation treatment. Therefore, 16 who suffered from ST-elevation myocardial infarction were investigated. Patients treated (100 mg/day, long-term) stopped taking (10 mg/day) Blood was collected at last day intake 1, 2, 5, 12 30 days later. Platelet in response to ADP...
The efficacy of mesenchymal stem cell infusion is currently tested in numerous clinical trials. However, therapy-induced thrombotic consequences have been reported several patients. aim this study was to optimize protocols for heterologous human adult liver-derived progenitor (HHALPC) infusion, order eliminate acute thrombogenesis liver-based metabolic or decompensated cirrhotic (ADC) In rats, effects were absent when HHALPCs infused at low dose (5 × 106 cells/kg), high 107 cells/kg)...