A5046792925- Extracellular vesicles in disease
- Blood Coagulation and Thrombosis Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Platelet Disorders and Treatments
- Inflammatory Biomarkers in Disease Prognosis
- Blood properties and coagulation
- MicroRNA in disease regulation
- Protease and Inhibitor Mechanisms
- Pregnancy and preeclampsia studies
- Atherosclerosis and Cardiovascular Diseases
- Cardiovascular Disease and Adiposity
- Hemoglobinopathies and Related Disorders
- Cell Adhesion Molecules Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Complement system in diseases
- Peripheral Neuropathies and Disorders
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Medical Imaging and Pathology Studies
- Systemic Sclerosis and Related Diseases
- Labor Movements and Unions
- Systemic Lupus Erythematosus Research
- Parasites and Host Interactions
- Long-Term Effects of COVID-19
- Single-cell and spatial transcriptomics
- Inflammasome and immune disorders
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Aix-Marseille Université
2016-2025
Inserm
2016-2025
Hôpital de la Timone
2022-2025
Alphabio
2023-2025
Hôpital de la Conception
2014-2023
Assistance Publique Hôpitaux de Marseille
2013-2023
Imperial College London
2023
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2019-2022
Center for Vascular Biology Research
2021
Abstract Purpose: Despite the strong association between malignant disease and thromboembolic disorders, molecular cellular basis of this relationship remains uncertain. We evaluated hypothesis that tumor-derived tissue factorbearing microparticles in plasma contribute to cancer-associated thrombosis. Experimental Design: developed impedance-based flow cytometry detect, quantitate, size platelet-poor plasma. number a cohort cancer patients different histologies (N = 96) conducted...
ABSTRACT Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application analysis EVs other submicron particles has presented many challenges produced number controversial results, in part due limitations instrument detection, lack robust methods ambiguities how data should be interpreted. These complications exacerbated by field's reporting framework, EV‐FC manuscripts...
Essentials Platelet extracellular vesicles (EVs) concentrations measured by flow cytometers are incomparable. A model is applied to convert ambiguous scatter units EV diameter in nanometer. Most included lack the sensitivity detect EVs of 600 nm and smaller. The outperforms polystyrene beads for comparability platelet concentrations.Background Detection cytometry has poor interlaboratory comparability, owing differences cytometer (FCM) sensitivity. Previous workshops distributed set a...
Abstract Coronavirus disease 2019 (COVID-19) has become one of the biggest public health challenges this century. Severe forms are associated with a thrombo-inflammatory state that can turn into thrombosis. Because tissue factor (TF) conveyed by extracellular vesicles (EVs) been implicated in thrombosis, we quantified EV-TF activity cohort hospitalized patients COVID-19 (n = 111) and evaluated its link inflammation, severity, thrombotic events. Patients severe were compared those who had...
Abstract Extracellular vesicles (EVs) are nanosized with a lipid bilayer that released from cells of the cardiovascular system, and considered important mediators intercellular extracellular communications. Two types EVs particular interest exosomes microvesicles, which have been identified in all tissue body fluids carry variety molecules including RNAs, proteins, lipids. potential for use diagnosis prognosis diseases as new therapeutic agents, particularly setting myocardial infarction...
Recent publications have demonstrated the presence of tissue factor (TF)–bearing microparticles (MPs) in blood patients suffering from cancer. However, whether these MPs are involved thrombosis remains unknown. We show that pancreatic and lung cancer cells produce express active TF P-selectin glycoprotein ligand 1 (PSGL-1). Cancer cell–derived aggregate platelets via a TF-dependent pathway. In vivo, MPs, but not their parent cells, infused into living mouse accumulate at site injury reduce...
Cellular microparticles (MP) are promising biomarkers in many pathological situations. Although flow cytometry (FCM) is widely used for their measurement, it has raised controversies because the smallest MP size falls below detection limit of standard FCM (sd-FCM). Following recent technological improvements leading to high sensitivity (hs-FCM), our objectives were (1) evaluate potential hs-FCM extended detection, (2) set up a standardized protocol enumeration, and (3) compare counts...
Objective To compare response to rituximab (RTX) between adult patients positive for myelin oligodendrocyte glycoprotein (MOG) and aquaporin‐4 (AQP4) antibodies. Methods We prospectively studied with MOG or AQP4 antibodies who received RTX under an individualized dosing schedule adapted the biological effect of monitored by memory B‐cell measurement. Memory B cells were counted monthly when relapse occurred. The was considered significant <0.05% in peripheral blood lymphocytes. Results In...
Abstract Beside the commonly described pulmonary expression of coronavirus disease 2019 (COVID-19), major vascular events have been reported. The objective this study was to investigate whether increased levels circulating endothelial cells (CECs) might be associated with severe forms COVID-19. Ninety-nine patients COVID-19 were enrolled in retrospective study. Patients intensive care units (ICU) had significantly higher CEC counts than non-ICU and extent injury correlated putative markers...
We recently assigned a new fibrinolytic function to cell-derived microparticles in vitro. In this study we explored the relevance of novel property vivo situation.Circulating were isolated from plasma patients with thrombotic thrombocytopenic purpura or cardiovascular disease and healthy subjects. Microparticles also obtained purified human blood cell subpopulations. The plasminogen activators on identified by flow cytometry enzyme-linked immunosorbent assays; their capacity generate plasmin...