A5006955960- Extracellular vesicles in disease
- Inflammatory Biomarkers in Disease Prognosis
- Blood Coagulation and Thrombosis Mechanisms
- Platelet Disorders and Treatments
- Blood properties and coagulation
- Angiogenesis and VEGF in Cancer
- Venous Thromboembolism Diagnosis and Management
- Complement system in diseases
- Medical Imaging and Pathology Studies
- Lymphoma Diagnosis and Treatment
- Caveolin-1 and cellular processes
- Cancer, Lipids, and Metabolism
- Occupational and environmental lung diseases
- T-cell and B-cell Immunology
- Wnt/β-catenin signaling in development and cancer
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac and Coronary Surgery Techniques
- Pleural and Pulmonary Diseases
- Prostate Cancer Treatment and Research
- Blood groups and transfusion
- Congenital heart defects research
- Multiple Sclerosis Research Studies
- Mitochondrial Function and Pathology
Aix-Marseille Université
2012-2024
Inserm
2012-2024
Hôpital de la Conception
2011-2024
Unité de recherche sur les maladies cardiovasculaires et métaboliques
2019-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2024
Alphabio
2024
Hôpital de la Timone
2023
Imperial College London
2023
Assistance Publique Hôpitaux de Marseille
2013-2016
Pulmonary Vascular Research Institute
2016
Objective To compare response to rituximab (RTX) between adult patients positive for myelin oligodendrocyte glycoprotein (MOG) and aquaporin‐4 (AQP4) antibodies. Methods We prospectively studied with MOG or AQP4 antibodies who received RTX under an individualized dosing schedule adapted the biological effect of monitored by memory B‐cell measurement. Memory B cells were counted monthly when relapse occurred. The was considered significant <0.05% in peripheral blood lymphocytes. Results In...
Platelets promote metastasis, however, their role in tumor growth remains controversial. Here, we investigated the effect of platelet interactions with colorectal cells. extravasated into microenvironment and interacted cells a cadherin-6-dependent manner. The interaction induced spreading, release granule content, generation three types microparticles (iMP) that expressed markers, or both. presence iMPs was confirmed cancer tissue specimens. significantly reduced increased intratumoral...
Background The activation of complement during platelet is incompletely understood. Objectives: We sought to explore the formation C5b-9 and anaphylatoxins binding collagen-activated platelets. Methods C5b-9, C3a, C4a C5a, anaphylatoxin receptors C3aR1 C5aR were measured by flow cytometry and/or confocal microscopy. Platelet microparticles quantified cytometry, their content was determined western blot analyses. In all experiments, sodium citrate used for blood anticoagulation. Results...
ABSTRACT Introduction The causes of cytopenias are numerous, and the bone marrow aspirate helps to identify them. In rare cases, these due metastases from solid cancers. techniques used in hematology laboratories limited characterizing cells. Interaction with cytopathology laboratory becomes critical for tumor cells completing a comprehensive diagnosis aspirate. Methods This article describes series 38 aspirates 36 patients bicytopenias who underwent aspiration whom hematologists sent sample...
The level of circulating platelet-, erythrocyte-, leucocyte- and endothelial-derived microparticles detected by high-sensitivity flow cytometry was investigated in 37 β-thalassaemia major patients receiving a regular transfusion regimen. phospholipid procoagulant potential the microparticle-dependent tissue factor activity were evaluated. A high erythrocyte- platelet-microparticles found. In contrast, number endothelial within normal range. Platelet significantly higher splenectomized than...
// Elisa Roca 1, 2, 3 , Romaric Lacroix 4 Coralie Judicone 5 Sophie Laroumagne 2 Stéphane Robert 1 Sylvie Cointe Alexandre Muller Elise Kaspi 6, 7 Patrice Roll Alain R. Brisson 8 Claudio Tantucci Philippe Astoul 9, * Françoise Dignat-George 4, VRCM, UMR-S1076, Aix-Marseille Université, INSERM, Faculté de Pharmacie, Marseille, France Division of Thoracic Oncology, Pleural Diseases, and Interventional Pulmonology, Hôpital Nord, AP-HM, Cattedra di Malattie dell'Apparato Respiratorio, Università...
The disruption of endothelial homeostasis is a major determinant in the pathogenesis systemic sclerosis (SSc) and reflected by soluble cellular markers activation, injury repair. We aimed to provide combined assessment delineate specific profiles associated with SSc disease its severity. conducted an observational, single-centre study comprising 45 patients 41 healthy control subjects. Flow cytometry was used quantify circulating microparticles (EMPs) CD34+ progenitor cell subsets....
Maintenance chemotherapy is an important part of the treatment ALL in children. It relies on long-term oral administration daily low-dose mercaptopurin and weekly methotrexate. Although it has been used clinic for decades, its mechanisms action remain unclear. Here, we investigated different angiogenic immune biomarkers to gain insights into maintenance therapy children with ALL. We thus monitored circulating endothelial cells (CEC), progenitor (EPC) microparticles (EMP), pro-angiogenic...
ABSTRACT Among extracellular vesicles, leukocyte‐derived microvesicles (LMVs) have emerged as complex vesicular structures. Primarily identified procoagulant entities, they were more recently ascribed to plasmin generation capacity (MV‐PGC). The objectives of this work (1) develop a new hybrid bio‐assay combining the specific isolation LMVs and measurement their PGC, compare its performance original method based on centrifugation, (2) validate MV‐PGC in septic shock, increased levels...
Microvesicles (MVs) have previously been shown to exert profibrinolytic capacity, which is increased in patients with septic shock (SS) a favorable outcome. We, therefore, hypothesized that the plasmin generation capacity (PGC) could confer MVs protective effect supported by their lyse thrombus, and we investigated mechanisms involved. Using an MV-PGC kinetic assay, ELISA, flow cytometry, found granulocyte (Gran-MVs) from SS display heterogeneous PGC profile driven uPA (urokinase)/uPAR...
CD34 + progenitor cells are growing in use for vascular repair. However, diabetic individuals with cardiovascular diseases, these have dysfunctional engraftment capabilities, which compromise their autologous cell therapy. The thrombospondin-1-derived peptide RFYVVMWK has previously been reported to stimulate adhesiveness through CD47 and integrin activation pathways. Our aim was test whether preconditioning could modulate phenotype enhance its proadhesive properties patients. Peripheral...
<b>Background:</b> Pleural biomarkers are needed for the diagnosis of malignant pleural effusion at an early stage before true carcinomatosis. Tumor cells produce microparticles, and, therefore, we hypothesized that tumor-derived microparticles could be present in liquid and help to identify patients with effusions by a non-invasive method. <b>Methods:</b> Fifty benign (n=11) or (n=39) were included this study. Among them, 39 consecutive histologically shown primary metastatic cancer...