A5059275904- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Lattice Boltzmann Simulation Studies
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Immune cells in cancer
- T-cell and B-cell Immunology
- Musculoskeletal synovial abnormalities and treatments
- Osteoarthritis Treatment and Mechanisms
- Lymphoma Diagnosis and Treatment
- Nanopore and Nanochannel Transport Studies
- Inflammasome and immune disorders
- Blood disorders and treatments
- Immune Response and Inflammation
- Tendon Structure and Treatment
- Immunotherapy and Immune Responses
- Icing and De-icing Technologies
- Nanoplatforms for cancer theranostics
- Blood groups and transfusion
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Cell death mechanisms and regulation
- Inflammatory mediators and NSAID effects
- Platelet Disorders and Treatments
- RNA Interference and Gene Delivery
Utrecht University
2016-2023
University of Cambridge
2018-2020
Addenbrooke's Hospital
2018
University Medical Center
2016
Sanquin
2011-2014
Amsterdam UMC Location University of Amsterdam
2010-2014
University of Amsterdam
2011-2014
Radboud University Nijmegen
2006
Radboud University Medical Center
2006
ABSTRACT Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application analysis EVs other submicron particles has presented many challenges produced number controversial results, in part due limitations instrument detection, lack robust methods ambiguities how data should be interpreted. These complications exacerbated by field's reporting framework, EV‐FC manuscripts...
To explore the involvement of synovial macrophages in early cartilage damage osteoarthritis (OA), and to identify role matrix metalloproteinase 3 (MMP-3) pathology late OA.The MMP-mediated OA was studied by depleting prior elicitation a collagenase-induced instability model OA. The expression MMP synovium monitored using TaqMan analysis. In spontaneous induced OA, scored MMP-3-knockout mice control mice, histologic assessment VDIPEN staining.On day 14 following induction neoepitopes were...
BACKGROUND. Cell-free circulating nucleic acids, including 22-nt microRNAs (miRNAs), represent noninvasive biomarkers for treatment response monitoring of cancer patients. While the majority plasma miRNA is bound to proteins, a smaller, less well-characterized pool associated with extracellular vesicles (EVs). Here, we addressed whether EV-associated miRNAs reflect metabolic disease in classical Hodgkin lymphoma (cHL)
Extracellular vesicles (EVs) in blood plasma are recognized as potential biomarkers for disease. Although is easily obtainable, analysis of EVs at the single particle level still challenging due to biological complexity this body fluid. Besides EVs, contains different types lipoproteins particles (LPPs), that outnumber by orders magnitude and which partially overlap biophysical properties such size, density molecular makeup. Consequently, during EV isolation LPPs often co-isolated....
Essentials Extracellular vesicles (EVs) in biological fluids are promising biomarkers for disease. Fluorescence-based flow cytometric analysis is suitable to detect low abundant EV subsets. Particles of non-interest can induce false-positive light scatter and fluorescent signals. Interference particles be monitored by analyzing serial dilutions.Background plasma increasingly being recognized as potential biomarkers. diagnostic purposes should robust allow subsets with a wide range abundance...
ABSTRACT Extracellular vesicles (EVs) in plasma are commonly identified by staining with antibodies and generic dyes, but the specificity of dyes to stain EVs is often unknown. Previously, we showed that platelet‐depleted platelet concentrate contains two populations particles >200 nm, one population a refractive index (RI) < 1.42 included majority EVs, second an RI > 1.42, which was thought include lipoproteins. In this study, investigated whether can be distinguished from...
During intervertebral disc ageing, chondrocyte-like cells (CLCs) replace notochordal (NCs). NCs have been shown to induce regenerative effects in CLCs. Since vesicles released by may be responsible for these effects, we characterized NC-derived extracellular (EVs) and determined their effect on EVs were purified from porcine NC-conditioned medium (NCCM) through size exclusion chromatography, ultracentrifugation or density gradient centrifugation. Additionally, the quantitatively analyzed...
ABSTRACT Features like small size, low refractive index and polydispersity pose challenges to the currently available detection methods for Extracellular Vesicles (EVs). In addition, lack of appropriate standards set up experimental conditions makes it difficult compare analyses obtained by different technical approaches. By modifying synthetic nanovesicles with recombinant antigenic regions EV‐enriched tetraspanins, we aimed construct an EV‐mimetic that can be used as a suitable standard EV...
Abstract Tumour hypoxia is a hallmark of solid tumours and contributes to tumour progression, metastasis development therapy resistance. In response hypoxia, cells secrete pro‐angiogenic factors induce blood vessel formation restore oxygen supply hypoxic regions. Extracellular vesicles (EVs) are emerging as mediators intercellular communication in the microenvironment. Here we demonstrate that increased expression LC3/GABARAP protein family member GABARAPL1, required for endosomal...
Extracellular vesicles (EVs) in synovial fluid (SF) are gaining increased recognition as important factors joint homeostasis, regeneration, and biomarkers of disease. A limited number studies have investigated EVs SF samples patients with disease, but knowledge on the role healthy joints is lacking. In addition, no standardized protocol available for isolation from SF. Based high viscosity caused by concentrations hyaluronic acid (HA) – a prominent extracellular matrix component it was...
Inflammation is the hallmark of most joint disorders. However, precise regulation induction, perpetuation, and resolution inflammation not entirely understood. Since extracellular vesicles (EVs) are critical for intercellular communication, we aim to unveil their role in these processes. Here, investigated EVs' dynamics phospholipidome profile from synovial fluid (SF) healthy equine joints horses with lipopolysaccharide (LPS)-induced synovitis. LPS injection triggered a sharp increase SF-EVs...
Abstract Flow cytometry allows multiparameter analysis on a single‐cell basis and is currently the method of choice to rapidly assess heterogeneity cell populations in suspension. With research field extracellular vesicles (EV) expanding, there an increased demand address EV biological samples. Although flow would be ideal technique do so, available instruments are general not equipped optimally detect dim light scatter signals generated by submicron‐sized particles like EV. sideward...
Apoptosis plays an essential role in the control of erythropoiesis under normal and pathological conditions. However, contribution individual proteins within cell death signalling pathways remains poorly defined. Here, we investigated pro-apoptotic Bcl-2 family member Noxa regulation erythropoiesis. We found that expression is induced during erythroid differentiation human murine precursor cells. Using vitro model systems for progenitors, observed rapid induction upon cytokine deprivation....
ABSTRACT Inflammation is the hallmark of most joint disorders. However, precise regulation induction, perpetuation, and resolution inflammation not entirely understood. Since extracellular vesicles (EVs) are critical for intercellular communication, we aim to unveil their role in these processes. Here, investigated EVs’ dynamics phospholipidome profile from synovial fluid (SF) healthy equine joints horses with lipopolysaccharide (LPS)–induced synovitis. LPS injection triggered a sharp...
Abstract Extracellular vesicles (EVs) in blood plasma are recognized as potential biomarkers for disease. Although is easily obtainable, analysis of EVs at the single particle level still challenging due to biological complexity this body fluid. Besides EVs, contains different types lipoproteins particles (LPPs), that outnumber by orders magnitude and which partially overlap biophysical properties such size, density molecular makeup. Consequently, during EV isolation LPPs often co-isolated....