A5037344212- Platelet Disorders and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Venous Thromboembolism Diagnosis and Management
- Nitric Oxide and Endothelin Effects
- Antiplatelet Therapy and Cardiovascular Diseases
- Erythrocyte Function and Pathophysiology
- Blood Coagulation and Thrombosis Mechanisms
- Advanced NMR Techniques and Applications
- Ion Transport and Channel Regulation
- Atomic and Subatomic Physics Research
- Electrolyte and hormonal disorders
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Renin-Angiotensin System Studies
- Solid-state spectroscopy and crystallography
- Blood properties and coagulation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Potassium and Related Disorders
- Asthma and respiratory diseases
- Hemoglobinopathies and Related Disorders
- Atrial Fibrillation Management and Outcomes
- Redox biology and oxidative stress
- Heme Oxygenase-1 and Carbon Monoxide
- Apelin-related biomedical research
- Bee Products Chemical Analysis
- Nutrition and Health Studies
Medical University of Białystok
2015-2024
Maastricht University Medical Centre
2018
University of Tübingen
2017
Patients suffering from chronic kidney disease (CKD) are at a 20-fold higher risk of dying due to cardiovascular diseases (CVDs), primarily thrombosis following vascular injury. CKD is connected with retention uremic toxins, especially indoxyl sulfate (IS), which currently considered as non-classical CKD-specific factor for CVDs. The present study aimed examine the effect exposure IS on hemostatic system and arterial in model without greater interferences milieu consisting additional toxins....
Chronic kidney disease (CKD) patients are at high risk for thrombotic events. Indoxyl sulfate (IS) is one of the most potent uremic toxins that accumulates during CKD. Even though IS associated with an increased cardiovascular disease, its impact on events still remains not fully understood. The purpose study was to evaluate direct effect process. We examined acute exposure thrombus development induced by electric current in Wistar rats, intravital formation after laser-induced injury mice...
Background: Adrenaline is believed to play a role in thrombosis and hemostasis. The complex effect of its clinically relevant concentrations on thrombus formation, coagulation fibrinolysis human blood has never been specifically studied. Methods: Confocal microscopy was used study formation under flow, exposure phosphatidylserine (PS) adhered platelets, evaluate clots density, measure kinetics fibrin external flow. Flow cytometry utilized assess PS non-adhered platelets. Kinetics clot...
Ultra-low-field (ULF) nuclear magnetic resonance (NMR) is a promising spectroscopy method allowing for, e.g., the simultaneous detection of multiple nuclei. To overcome low signal-to-noise ratio that usually hampers wider application, we present here an alternative approach to ULF NMR, which makes use hyperpolarizing technique signal amplification by reversible exchange (SABRE). In contrast standard parahydrogen hyperpolarization, SABRE can continuously hyperpolarize 1 H as well other...
We tested the hypothesis that hypertension associated with polycythemia vera (PV) may be related to hemoglobin released from erythrocytes (cell-free hemoglobin, fHb). assessed hematocrit, mean arterial pressure (MAP), blood viscosity, and level of fHb nitrite/nitrate (NOx) in plasma 73 PV patients 38 healthy controls. The effect isovolemic erythrocytapheresis (ECP) on considered parameters was also studied. From whole group a subset subjects normal (normotensive patients, n = 16) elevated...
The aim of this study was to assess whether steroid-naïve asthma modulates hemostasis. We evaluated the clot retraction rate (CRR), fibrinolysis (FR), density (CD) (by confocal microscopy), plasma levels plasminogen activator inhibitor (PAI-1), and factor XIII (FXIII), NO in exhaled breath (FENO ), spirometry (FEV1 ) eosinophil count (EOS) 36 patients with allergic, 34 healthy controls. observed significantly (P < 0.001) reduced CRR, FR, FEV1 increased FENO , EOS, PAI-1, FXIII, CD compared...
To design new material for blood-related applications one needs to consider various factors such as cytotoxicity, platelet adhesion, or anti-thrombogenic properties. The aim of this work is the new, highly effective materials possessing high blood compatibility. do this, composites based on poly(vinylidene fluoride) (PVDF) support covered with a single-walled carbon nanohorns (CNHs) layer were prepared. PVDF-CNHs subsequently used first time in hemocompatibility studies. raise never applied...
The aim of this study was to establish the role platelets and activated factor XIIIa (FXIIIa) in structuring fibrin network as well clarify effect compaction on clot lysis.  Turbidimetry used for one-stage clotting test where PFP is regarded single factor-deficient plasma (platelets lacking factor) autologous PRP deficiency corrected plasma. Structural features developed subsequently lysed network, formed under static flow conditions, were visualized by confocal microscopy....
Rise in mean platelet volume (MPV) has been demonstrated to be associated with increased reactivity. In diabetes patients, augmented MPV was proposed contribute risk of thrombotic complications. Therefore, the aim this study investigate whether under hyperglycemic conditions, aldose reductase (AR)-mediated sorbitol formation and rise cell volume, which subsequently results hyperactivation. Platelets were obtained from 30 healthy volunteers 13 patients diabetes. We evaluated changes size,...
Asthma enhances the risk of pulmonary embolism. The mechanism this phenomenon is unclear.We evaluated kinetics clot formation, retraction rate (CRR), volume at 40 min, lactate production (a marker aerobic glycolysis in platelets contracting clots), blood eosinophil count (EOS), nitric oxide exhaled breath (FENO), and spirometry (FEV1) 50 healthy controls 81 allergic asthmatics (41 subjects with steroid-naïve asthma steroid-treated asthma).Thromboelastometry revealed that only had slightly...
In our previous study, we introduced the platelet endothelial cell adhesion molecule 1 (PECAM-1)/thrombus ratio, which is a parameter indicating proportion of PECAM-1 in laser-induced thrombi mice. Because an antithrombotic molecule, higher PECAM-1/thrombus less activated platelets. this used extracorporeal model thrombosis (flow chamber model) to verify its usefulness assessment ratio animal and human studies. Using lipopolysaccharide (LPS)-induced inflammation model, also evaluated whether...
Using porcine blood, we examined the impact of hypochlorite, product activated inflammatory cells, on clot retraction (CR), an important step hemostasis. We found that, in vitro, HOCl is able to reduce CR rate and enlarge final size whole blood (t.c. 100 μM), platelet-rich plasma (PRP) threshold concentration 50 artificial system (washed platelets fibrinogen) 25 nM). Combination low peroxynitrite concentrations resulted synergistic inhibition by these stressors. Concentrations completely...
This study was undertaken to establish the presence and role of aquaporins (AQPs) in human platelets. Immunodetection with polyclonal antibodies fluorescent microscopy suggest AQP isoforms – 0–7 9–12 localized (in resting platelets) plasma membrane dense alpha granules. In thrombin- or monensin-treated platelets, granules’ AQPs become visible whole cell body, indicating swelling. our studies on platelet responses we used tetrachloroauric acid (HAuCl4), a classical water channel blocker. We...
Abstract Sodium-glucose cotransporter 2 inhibitors (SGLT2i) are antihyperglycemic drugs that decrease mortality from cardiovascular diseases. However, their effects on hemostasis in the cardioprotective have not been evaluated. Therefore, of canagliflozin (CANA, 100 mg/kg, p.o. ) and dapagliflozin (DAPA, 10 parameters were investigated female male normoglycemic streptozotocin (180 i.p. )-induced diabetic mice. CANA DAPA reduced platelet activity thrombus mice both diabetic. decreased...
Background: Recent studies indicate that aquaporin water channels (AQPs) have a regulatory function in human platelet secretion and procoagulant response of murine platelets. However, the engagement AQPs morphological changes, thrombus formation blood has never been investigated. Methods: Confocal microscopy was used to study spreading, filopodia formation, ballooning, under flow. Flow cytometry utilized assess phosphatidylserine (PS) exposure microparticles shedding. Kinetics clot vitro...