A5032342739- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Venous Thromboembolism Diagnosis and Management
- Electrolyte and hormonal disorders
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Ion Transport and Channel Regulation
- Potassium and Related Disorders
- Osteoarthritis Treatment and Mechanisms
- Blood properties and coagulation
- RNA Interference and Gene Delivery
- Mesenchymal stem cell research
- Pomegranate: compositions and health benefits
- Hormonal Regulation and Hypertension
- Blood Coagulation and Thrombosis Mechanisms
- Nitric Oxide and Endothelin Effects
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Blood Pressure and Hypertension Studies
- Reproductive Biology and Fertility
- Antioxidant Activity and Oxidative Stress
- Cancer-related molecular mechanisms research
- Global Education Systems and Policies
- MicroRNA in disease regulation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Atherosclerosis and Cardiovascular Diseases
- Management and Organizational Practices
Medical University of Białystok
2019-2024
Gdańsk University of Technology
2015
Background: Adrenaline is believed to play a role in thrombosis and hemostasis. The complex effect of its clinically relevant concentrations on thrombus formation, coagulation fibrinolysis human blood has never been specifically studied. Methods: Confocal microscopy was used study formation under flow, exposure phosphatidylserine (PS) adhered platelets, evaluate clots density, measure kinetics fibrin external flow. Flow cytometry utilized assess PS non-adhered platelets. Kinetics clot...
Abstract Chondrocytes isolated from auricular cartilage of 7‐day‐old rabbits were grown in vitro until the onset phase III, occurring after 10‐14 population doublings (PD). The size cells and their dry mass measured at various PD levels. These data compared with results analogous measurements chondrocytes freshly 28‐day‐old rabbits. Both vivo, during growth, vitro, some increased considerably acquired two nuclei. cultured for 4 still capable depositing elastic fibers culture forming...
The aim of this study was to establish the role platelets and activated factor XIIIa (FXIIIa) in structuring fibrin network as well clarify effect compaction on clot lysis.  Turbidimetry used for one-stage clotting test where PFP is regarded single factor-deficient plasma (platelets lacking factor) autologous PRP deficiency corrected plasma. Structural features developed subsequently lysed network, formed under static flow conditions, were visualized by confocal microscopy....
This study was undertaken to establish the presence and role of aquaporins (AQPs) in human platelets. Immunodetection with polyclonal antibodies fluorescent microscopy suggest AQP isoforms – 0–7 9–12 localized (in resting platelets) plasma membrane dense alpha granules. In thrombin- or monensin-treated platelets, granules’ AQPs become visible whole cell body, indicating swelling. our studies on platelet responses we used tetrachloroauric acid (HAuCl4), a classical water channel blocker. We...
Background: Recent studies indicate that aquaporin water channels (AQPs) have a regulatory function in human platelet secretion and procoagulant response of murine platelets. However, the engagement AQPs morphological changes, thrombus formation blood has never been investigated. Methods: Confocal microscopy was used to study spreading, filopodia formation, ballooning, under flow. Flow cytometry utilized assess phosphatidylserine (PS) exposure microparticles shedding. Kinetics clot vitro...
In our previous study, we showed that ellagitannin- and procyanidin-rich tormentil extract (TE) decreased experimental arterial thrombosis in normoglycemic rats through platelet inhibition. TE also slightly increased coagulation attenuated fibrinolysis; however, these effects did not nullify the antithrombotic effect of TE. The present study aimed to assess whether exerts activity streptozotocin (STZ)-induced diabetes, which is characterized by pre-existing impaired fibrinolysis, vivo ex...
Adrenaline has recently been found to trigger phosphatidylserine (PS) exposure on blood platelets, resulting in amplification of the coagulation process, but mechanism is only fragmentarily established. Using a panel platelet receptors' antagonists and modulators signaling pathways, we evaluated importance these adrenaline-evoked PS by flow cytometry. Calcium sodium ion influx into cytosol, after adrenaline treatment, was examined fluorimetric measurements. We strong reduction blocking...
During pathogen invasion, activated neutrophils secrete myeloperoxidase (MPO), which generates high local concentrations of hypochlorous acid (HOCl), a strong antimicrobial agent. Prolonged or uncontrolled HOCl production may, however, affect hemostasis, manifesting in inhibition platelet aggregation and thrombus formation elevated fibrin density attenuated fibrinolysis. In this report, we investigated whether three plant-derived polyphenols with well-known antioxidant properties, i.e.,...
Streszczenie Zakrzepy i zatory stanowią obecnie główną bezpośrednią przyczynę zgonów u hospitalizowanych pacjentów w krajach rozwiniętych. Szczególnie istotną rolę procesie krzepnięcia, jak również stanach patologicznej nadkrzepliwości, odgrywają płytki krwi. Jednym z fizjologicznych aktywatorów płytek krwi o stosunkowo mało poznanym mechanizmie działania jest adrenalina (epinefryna). Co istotne, warunki predysponujące do pojawienia się we podwyższonego stężenia adrenaliny korelują ze...