A5038465935- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Sepsis Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- HIV/AIDS Research and Interventions
- HIV Research and Treatment
- Viral Infections and Outbreaks Research
- SARS-CoV-2 and COVID-19 Research
- HIV/AIDS drug development and treatment
- Respiratory Support and Mechanisms
- HIV-related health complications and treatments
- Pneumonia and Respiratory Infections
- Respiratory viral infections research
- Vaccine Coverage and Hesitancy
- Venous Thromboembolism Diagnosis and Management
- Pleural and Pulmonary Diseases
- Hepatitis B Virus Studies
- COVID-19 and healthcare impacts
- Pulmonary Hypertension Research and Treatments
- Hepatitis C virus research
- Influenza Virus Research Studies
- Virology and Viral Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Long-Term Effects of COVID-19
- Leptospirosis research and findings
Erasmus MC
2016-2025
Erasmus University Rotterdam
2016-2025
Misgav Ladach
2022
Universidad de Salamanca
2022
Rotterdam University of Applied Sciences
2015
Leiden University
2015
University of Applied Sciences Leiden
2015
Slotervaartziekenhuis
2002-2012
Academic Medical Center
2003-2012
Netherlands Organisation for Health Research and Development
2012
SARS-CoV-2 has been identified as the causative agent of a global outbreak respiratory tract disease (COVID-19). In some patients infection results in moderate to severe acute distress syndrome (ARDS), requiring invasive mechanical ventilation. High serum levels IL-6, IL-10 and an immune hyperresponsiveness referred 'cytokine storm' have associated with poor clinical outcome. Despite large numbers COVID-19 cases deaths, information on phenotype kinetics SARS-CoV-2-specific T cells is...
Key questions in COVID-19 are the duration and determinants of infectious virus shedding. Here, we report that shedding is detected by cultures 23 129 patients (17.8%) hospitalized with COVID-19. The median 8 days post onset symptoms (IQR 5-11) drops below 5% after 15.2 (95% confidence interval (CI) 13.4-17.2). Multivariate analyses identify viral loads above 7 log10 RNA copies/mL (odds ratio [OR] 14.7 (CI 3.57-58.1; p < 0.001) as independently associated isolation SARS-CoV-2 from...
ABSTRACT Background Long-term shedding of viral RNA in COVID-19 prevents timely discharge from the hospital or de-escalation infection prevention and control practices. Key questions are duration determinants infectious virus shedding. We assessed these using cultures respiratory tract samples hospitalized patients as a proxy for Methods Clinical virological data were obtained 129 (89 intensive care, 40 medium care). Generalized estimating equations used to identify if load, detection...
In July 2022, the ongoing monkeypox (MPX) outbreak was declared a public health emergency of international concern. Modified vaccinia Ankara-Bavarian Nordic (MVA-BN, also known as Imvamune, JYNNEOS or Imvanex) is third-generation smallpox vaccine that authorized and in use against MPX. To date, there are no data showing MPX virus (MPXV)-neutralizing antibodies vaccinated individuals nor efficacy Here we show MPXV-neutralizing can be detected after MPXV infection historic vaccination....
In the absence of a vaccine and therapeutic agent, personal hygiene physical distancing are essential measures to contain coronavirus disease 2019 pandemic.To determine whether social media campaign, targeted at gaps in behavior on distributed nationwide via digital news media, may be an effective method improve help inhibit person-to-person transmission severe acute respiratory syndrome 2.This survey study was designed uncover self-reported regarding Netherlands. A diagnostic by large...
There is a lack of validated tools to assess potential disease progression and hospitalisation decisions in patients presenting the emergency department (ED) with suspected infection. This study aimed identify suitable blood biomarkers (MR-proADM, PCT, lactate CRP) or clinical scores (SIRS, SOFA, qSOFA, NEWS CRB-65) fulfil this unmet need. An observational derivation patient cohort by an independent secondary analysis across nine EDs. Logistic Cox regression, area under receiver operating...
Abstract COVID-19 is associated with lymphopenia and ‘cytokine storm’, but no information available on specific cellular immune responses to SARS-CoV-2. Here, we characterized SARS-CoV-2-specific CD4 + CD8 T-cells in patients acute respiratory distress syndrome. The spike protein (S) proved a potent T-cell antigen predominantly produced Th1 cytokines. These novel data are important vaccine design will facilitate evaluation of candidate immunogenicity.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of disease 2019 (COVID-19). Little known about interplay between preexisting immunity to endemic seasonal coronaviruses and development a SARS-CoV-2-specific IgG response. We investigated kinetics, breadth, magnitude, level cross-reactivity antibodies against SARS-CoV-2 heterologous epidemic at clonal in patients with mild or severe COVID-19 as well control patients. assessed antibody reactivity nucleocapsid spike...
Patients with inborn errors of immunity (IEI) are at increased risk severe coronavirus disease-2019 (COVID-19). Effective vaccination against COVID-19 is therefore great importance in this group, but little known about the immunogenicity vaccines these patients.We sought to study humoral and cellular immune responses after mRNA-1273 adult patients IEI.In a prospective, controlled, multicenter study, 505 IEI (common variable immunodeficiency [CVID], isolated or undefined antibody...
Lower respiratory tract (LRT) disease induced by severe acute syndrome coronavirus 2 (SARS-CoV-2) can deteriorate to distress (ARDS). Because the release of neutrophil extracellular traps (NETs) is implicated in ARDS pathogenesis, we investigated presence NETs and correlates pathogenesis blood LRT samples critically ill patients with COVID-19. Plasma NET levels peaked early after intensive care unit admission were correlated SARS-CoV-2 RNA load sputum neutrophil-recruiting chemokines...
Patients with a severe COVID-19 infection often require admission at an intensive care unit (ICU) when they develop acute respiratory distress syndrome (ARDS). Hyperinflammation plays important role in the development of ARDS COVID-19. Procalcitonin (PCT) is biomarker which may be predictor hyperinflammation. When patients are emergency department (ED), elevated PCT levels could associated infections. The goal this study to investigate association between and infections ED.This was...
Background Severe dengue virus (DENV) disease is associated with extensive immune activation, characterized by a cytokine storm. Previously, elevated lipopolysaccharide (LPS) levels in were found to correlate clinical severity. In the present cross-sectional study we identified markers of microbial translocation and which are severe manifestations DENV infection. Methods Serum samples from DENV-infected patients collected during outbreak 2010 State São Paulo, Brazil. Levels LPS, binding...
Background During a dengue outbreak on the Caribbean island Aruba, highly elevated levels of ferritin were detected in virus infected patients. Ferritin is an acute-phase reactant and hyperferritinaemia hallmark diseases caused by extensive immune activation, such as haemophagocytic lymphohistiocytosis. The aim this study was to investigate whether patients associated with clinical markers activation coagulation disturbances. Methodology/Principal Findings Levels ferritin, standard...
The Netherlands has provided unrestricted access to direct-acting antivirals (DAAs) since November 2015. We analyzed the nationwide hepatitis C virus (HCV) treatment uptake among patients coinfected with human immunodeficiency (HIV) and HCV.Data were obtained from ATHENA HIV observational cohort in which >98% of HIV-infected ever registered 1998 are included. Patients included if they had 1 positive HCV RNA result, did not have spontaneous clearance, known still be care. Treatment outcome...
As several COVID-19 vaccines are rolled-out globally, it has become important to develop an effective strategy for vaccine acceptance, especially in high-risk groups, such as elderly. Vaccine misconception was declared by WHO one of the top 10 health issues 2019. Here we test effectiveness applying debunking combat misinformation, and reduce hesitancy.Participants were recruited via a daily news show on Dutch Television, targeted elderly viewers. The study conducted 980 citizens during...