A5037034052- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Respiratory viral infections research
- Rabies epidemiology and control
- Bacillus and Francisella bacterial research
- Vaccine Coverage and Hesitancy
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- COVID-19 and healthcare impacts
- Herpesvirus Infections and Treatments
- Immune Cell Function and Interaction
- Peripheral Neuropathies and Disorders
- Influenza Virus Research Studies
- Virology and Viral Diseases
- COVID-19 epidemiological studies
- Immune Response and Inflammation
- Immune responses and vaccinations
- Cancer Immunotherapy and Biomarkers
Erasmus MC
2016-2025
Erasmus University Rotterdam
2016-2025
Rotterdam University of Applied Sciences
2023
European Clinical Research Infrastructure Network
2021-2022
European Research Infrastructure on Highly Pathogenic Agents
2021-2022
Erasmus MC Cancer Institute
2022
Erasmus MC Transplant Institute
2022
Misgav Ladach
2022
Universidad de Salamanca
2022
Utrecht University
2019-2020
Abstract A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed detection of SARS-CoV-2 neutralizing, spike protein–specific, nucleocapsid-specific antibodies. Using serum samples from patients...
Two-way transmission on mink farms Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a zoonotic virus—one that spilled over from another species to infect and transmit among humans. We know humans can other animals with SARS-CoV-2, such as domestic cats even tigers in zoos. Oude Munnink et al. used whole-genome sequencing show SARS-CoV-2 infections were rife the southeastern Netherlands, all of which are destined be closed by March 2021 (see Perspective Zhou Shi). Toward end...
Key questions in COVID-19 are the duration and determinants of infectious virus shedding. Here, we report that shedding is detected by cultures 23 129 patients (17.8%) hospitalized with COVID-19. The median 8 days post onset symptoms (IQR 5-11) drops below 5% after 15.2 (95% confidence interval (CI) 13.4-17.2). Multivariate analyses identify viral loads above 7 log10 RNA copies/mL (odds ratio [OR] 14.7 (CI 3.57-58.1; p < 0.001) as independently associated isolation SARS-CoV-2 from...
Infection- or vaccination-induced SARS-CoV-2 S–specific CD4 + T cells are indifferent to the S mutations of variants concern.
Although dendritic cells (DCs) play an important role in mediating protection against influenza virus, the precise of lung DC subsets, such as CD11b− and CD11b+ conventional DCs or plasmacytoid (pDCs), different compartments is currently unknown. Early after intranasal infection, tracheal CD11b−CD11chi migrated to mediastinal lymph nodes (MLNs), acquiring co-stimulatory molecules process. This emigration from was followed by accumulation CD11b+CD11chi trachea interstitium. In MLNs, contained...
The severe acute respiratory distress syndrome coronavirus 2 (SARS-CoV-2) Omicron variant is spreading rapidly, even in vaccinated individuals, raising concerns about immune escape. Here, we studied neutralizing antibodies and T cell responses targeting SARS-CoV-2 D614G [wild type (WT)] the Beta, Delta, variants of concern a cohort 60 health care workers after immunization with ChAdOx-1 S, Ad26.COV2.S, mRNA-1273, or BNT162b2. High binding antibody levels against WT spike (S) were detected 28...
Abstract The world is entering a new era of the COVID-19 pandemic in which there an increasing call for reliable antibody testing. To support decision making on deployment serology either population screening or diagnostics, we present detailed comparison serological assays. We show that among selected assays wide diversity assay performance different scenarios and when correlated to virus neutralizing antibodies. Wantai ELISA detecting total immunoglobulins against receptor binding domain...
Tertiary lymphoid organs (TLOs) are organized aggregates of B and T cells formed in postembryonic life response to chronic immune responses infectious agents or self-antigens. Although CD11c+ dendritic (DCs) consistently found regions TLO, their contribution TLO organization has not been studied detail. We that CD11c(hi) DCs essential for the maintenance inducible bronchus-associated tissue (iBALT), a form induced lungs after influenza virus infection. Elimination had cleared from lung...
ABSTRACT Background Long-term shedding of viral RNA in COVID-19 prevents timely discharge from the hospital or de-escalation infection prevention and control practices. Key questions are duration determinants infectious virus shedding. We assessed these using cultures respiratory tract samples hospitalized patients as a proxy for Methods Clinical virological data were obtained 129 (89 intensive care, 40 medium care). Generalized estimating equations used to identify if load, detection...
Abstract A new coronavirus, SARS-CoV-2, has recently emerged to cause a human pandemic. Whereas molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated important for contact tracing, identifying the viral reservoir and epidemiological studies. Here, we developed serological detection of SARS-CoV-2 neutralizing, spike- nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed infections other...
Structured abstract for full paper Background After recovery from COVID-19, most patients have anti-SARS-CoV-2 neutralizing antibodies. Their convalescent plasma could be an inexpensive and widely available treatment COVID-19. Methods The Convalescent-plasma-for-COVID (ConCOVID) study was a randomized trial comparing with standard of care therapy in hospitalized COVID-19 the Netherlands. Patients were 1:1 received 300ml antibody titers at least 1:80. primary endpoint day-60 mortality key...
In July 2022, the ongoing monkeypox (MPX) outbreak was declared a public health emergency of international concern. Modified vaccinia Ankara-Bavarian Nordic (MVA-BN, also known as Imvamune, JYNNEOS or Imvanex) is third-generation smallpox vaccine that authorized and in use against MPX. To date, there are no data showing MPX virus (MPXV)-neutralizing antibodies vaccinated individuals nor efficacy Here we show MPXV-neutralizing can be detected after MPXV infection historic vaccination....
In kidney patients COVID-19 is associated with severely increased morbidity and mortality. A comprehensive comparison of the immunogenicity, tolerability, safety vaccination in different cohorts a control cohort lacking.This investigator driven, prospective, controlled multicenter study included 162 participants chronic disease (CKD) stages G4/5 (eGFR < 30 mL/min/1.73m2), 159 on dialysis, 288 transplant recipients, 191 controls. Participants received 2 doses mRNA-1273 vaccine (Moderna). The...
Abstract In a randomized clinical trial of 86 hospitalized COVID-19 patients comparing standard care to treatment with 300mL convalescent plasma containing high titers neutralizing SARS-CoV-2 antibodies, no overall benefit was observed. Using comprehensive translational approach, we unravel the virological and immunological responses following disentangle which may should be focus future studies. Convalescent is safe, does not improve survival, has effect on disease course, nor enhance viral...
The COVID-19 pandemic necessitates better understanding of the kinetics antibody production induced by infection with SARS-CoV-2. We aimed to develop a high-throughput multiplex assay detect antibodies SARS-CoV-2 assess immunity virus in general population.Spike protein subunits S1 and receptor binding domain, nucleoprotein were coupled microspheres. Sera collected before emergence (n = 224) non-SARS-CoV-2 influenza-like illness 184), laboratory-confirmed cases 115) various severities tested...
The emergence and rapid spread of SARS-CoV-2 variants may affect vaccine efficacy substantially. Omicron variant termed BA.2, which differs substantially from BA.1 based on genetic sequence, is currently replacing in several countries, but its antigenic characteristics have not yet been assessed. Here, we used cartography to quantify visualize differences between early (614G, Alpha, Beta, Gamma, Zeta, Delta, Mu) using hamster antisera obtained after primary infection. We first verified that...
The Ad26.COV2.S vaccine, which was approved as a single-shot immunization regimen, has been shown to be effective against severe coronavirus disease 2019. However, this vaccine induces lower acute respiratory syndrome 2 (SARS-CoV-2) spike protein (S)-specific antibody levels than those induced by messenger RNA (mRNA)-based vaccines. immunogenicity and reactogenicity of homologous or heterologous booster in persons who have received an priming dose are unclear.In single-blind, multicenter,...
The role of estrogens in ischemic heart disease (IHD) is uncertain. Evidence suggests that genetic variations the estrogen receptor alpha (ESR1) gene may influence IHD risk, but common sequence ESR1 unclear.To determine whether haplotype created by c.454-397T>C (PvuII) and c.454-351A>G (XbaI) polymorphisms associated with myocardial infarction (MI) risk.In 2617 men 3791 postmenopausal women from Rotterdam Study (enrollment between 1989-1993 follow-up to January 2000), a population-based,...