A5039747786- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Pneumonia and Respiratory Infections
- Cervical Cancer and HPV Research
- Viral gastroenteritis research and epidemiology
- Neonatal Respiratory Health Research
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Bacterial Infections and Vaccines
- Immunotherapy and Immune Responses
- Congenital Diaphragmatic Hernia Studies
- Immunodeficiency and Autoimmune Disorders
- Genital Health and Disease
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Tracheal and airway disorders
- SARS-CoV-2 detection and testing
- Inflammatory Myopathies and Dermatomyositis
- Complement system in diseases
- Animal Virus Infections Studies
- COVID-19 epidemiological studies
- Animal health and immunology
- Dupuytren's Contracture and Treatments
- Biosensors and Analytical Detection
National Institute for Public Health and the Environment
2013-2025
Eindhoven Cancer Registry
2020
Amsterdam UMC Location Vrije Universiteit Amsterdam
2013
Amsterdam UMC Location University of Amsterdam
2013
University Medical Center
2013
University Hospital and Clinics
2013
University Medical Center Utrecht
2009-2013
Screen
2013
NTL Institute for Applied Behavioral Science
2009
Groene Hart Ziekenhuis
2009
The COVID-19 pandemic necessitates better understanding of the kinetics antibody production induced by infection with SARS-CoV-2. We aimed to develop a high-throughput multiplex assay detect antibodies SARS-CoV-2 assess immunity virus in general population.Spike protein subunits S1 and receptor binding domain, nucleoprotein were coupled microspheres. Sera collected before emergence (n = 224) non-SARS-CoV-2 influenza-like illness 184), laboratory-confirmed cases 115) various severities tested...
Background We aimed to detect SARS-CoV-2 serum antibodies in the general population of Netherlands and identify risk factors for seropositivity amidst first COVID-19 epidemic wave. Methods Participants (n=3207, aged 2–90 years), enrolled from a previously established nationwide serosurveillance study, provided self-collected fingerstick blood sample completed questionnaire (median inclusion date 3 April 2020). IgG targeted against spike S1-protein were quantified using validated...
Abstract Respiratory syncytial virus (RSV) infection is a leading cause of hospitalization in infants. Underlying risk factors for RSV the general population are not well understood, as previous work has focused on severe outcomes clinical setting. Here we use RSV-specific IgG and IgA antibody measurements from two population-based cross-sectional serosurveys carried out Netherlands (n = 682) to classify children up 5 years seronegative or seropositive. We employ generalized additive model...
Respiratory syncytial virus (RSV) is a common global respiratory that increasingly recognized as major pathogen in frail older adults and cause of chronic obstructive pulmonary disease (COPD) exacerbations. There no single test for RSV has acceptable diagnostic accuracy. Trials vaccines have recently shown excellent safety efficacy against adults; defining the frequency RSV-related community infections COPD exacerbations important vaccine deployment decisions.
<h3>Objectives</h3> To compare the immunogenicity and safety of bivalent human papillomavirus (HPV)16/18 vaccine between female patients with juvenile idiopathic arthritis (JIA) healthy adolescents. <h3>Methods</h3> 68 55 girls aged 12–18 years were included in a prospective controlled observational cohort vaccinated at 0, 1 6 months. Primary outcomes expressed as seropositivity rate after three doses 7 12 months HPV-specific geometric mean antibody concentrations. Secondary...
In order to assess HPV-specific IgG characteristics, we evaluated multiple aspects of the humoral antibody response that will provide insight in HPV immune induced by infection and vaccination.Cross-reactivity antibodies or vaccination was assessed with VLP16 18 inhibition using a VLP-based multiplex immunoassay (MIA) for HPV16, 18, 31, 33, 45, 52 58. HPV16/18 specific IgG1-4 subclasses avidity were determined VLP-MIA sera after vaccination. Neutralizing small subset single-seropositive...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has led to considerable morbidity/mortality worldwide, but most infections, especially among children, have a mild course. However, it remains largely unknown whether infected children develop cellular immune memory.To determine memory T cell response is being developed, we performed longitudinal assessment of the SARS-CoV-2-specific by IFN-γ ELISPOT and activation marker analyses peripheral blood samples from unvaccinated adults...
Background In 2002 a Meningococcal serogroup C (MenC) conjugate vaccine, with tetanus toxoid as carrier protein, was introduced in the Netherlands single-dose at 14 months of age. A catch-up campaign performed targeting all individuals aged to 18 years. We determined MenC-specific immunity before and after introduction MenC (MenCC) vaccine. Methods Findings Two cross-sectional population-based serum banks, collected 1995/1996 (n = 8539) 2006/2007 6386), were used for this study. The main...
Asylum seekers are a vulnerable population for contracting infectious diseases. Outbreaks occur among children and adults. In the Netherlands, asylum seeker offered vaccination according to National Immunization Program. Little is known about protection against vaccine-preventable diseases (VPD) in adult seekers. this 2016 study, we assessed immunity of nine VPD identify groups that might benefit from additional vaccinations. We invited Syria, Iran, Iraq, Afghanistan, Eritrea Ethiopia...
Antibodies in human milk protect infants against infections, but currently no assay is described that able to simultaneously measure all 9 antibody isotypes and subclasses immunoglobulins fluids, such as milk. Our cohort "Protecting Respiratory tract Infections through Milk Analysis" (PRIMA) focused on the relation between occurrence of respiratory infections during first year life concentration maternal antibodies breastfeeding. We developed successfully validated a multiplex nine plasma...
Question Past research suggested respiratory syncytial virus (RSV) specific serum immunoglobulin A (IgA) antibodies as a biomarker of previous RSV infections in young infants accounting for maternal immunity. This study aimed to confirm this association, and establish serological threshold discriminating RSV-naïve from those who have experienced RSV. Material Methods involves 135 the LoewenKIDS Study with nasal swabs collected at each acute infection, samples ages 1 2. presence was...
The close genetic relationship of human and animal strains norovirus has raised the possibility transmission noroviruses from animals to humans may explain emergence certain strains. To assess if exposure bovine (NoV) might result in infection humans, an enzyme immunoassay (EIA) was designed validated order detect antibodies against norovirus. This two other EIAs were used test sera 210 veterinarians 630 matched population controls for IgG IgA recombinant capsid protein NoV (rBoV), Norwalk...
The bivalent HPV16/18 vaccine induces high antibody concentrations in serum while data about responses the cervix are limited. In this study, we investigated pre- and post-vaccination against seven high-risk HPV types by detection of IgG IgA HPV-specific antibodies cervical secretion samples (CVS) serum. From an monitoring study CVS were available (pre-vaccination (n = 297), one year 211) two years 141) post-dose-one vaccination) from girls aged 14-16 y. vaccinated with at months 0, 1 6. was...
Respiratory syncytial virus (RSV) generally causes mild disease but can cause severe infections in (premature) infants and elderly adults. Here, we studied RSV-specific antibody concentrations throughout life with emphasis on chronic obstructive pulmonary (COPD) patients.
We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae b polysaccharide (HibPS). The monoplex HibPS assay was compared a HibPS-specific competitive enzyme-linked immunosorbent showed good correlation (R=0.96). Furthermore, no cross-reactivity between four serogroups detected. This pentaplex Hib MIA is useful tool investigate serological responses different...
Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infections in infants, and there no vaccine available. In early life, the most important contributors to protection against infectious diseases are innate immune response maternal antibodies. However, antibody-mediated RSV disease incompletely understood, as both antibody levels neutralisation capacity correlate poorly with protection. Since antibodies also mediate natural killer (NK) cell activation, we...
Abstract Background: Men who have sex with men (MSM), in particular HIV-infected MSM, are at increased risk for diseases related to human papilloma virus (HPV). Our goal was assess the effect of HIV status on presence type-specific antibodies against seven high-risk HPV types HPV-unvaccinated MSM. Moreover, we compared determinants seropositivity between HIV-negative and Methods: MSM ≥18 years age were recruited from Amsterdam Cohort Studies, a sexually transmitted infection clinic, an...
In view of vaccine and monoclonal development to reduce hospitalization death due lower respiratory tract infection caused by RSV, assessment antibody levels against RSV is essential. This newly developed multiplex immunoassay able measure five proteins simultaneously. can provide valuable insight into the dynamics (maternal) in infants toddlers during first few years life, when primary occurs.
This study aimed to assess the safety and immunogenicity of quadrivalent human papillomavirus (qHPV) vaccination in childhood-onset systemic lupus erythematosus (cSLE) patients.Volunteer cSLE patients aged 9-20 years healthy controls (HC) were enrolled receive a two- or three-dose qHPV schedule from March 2014 2016. Study visits performed before first dose, one month after second third doses year dose. In each visit, disease activity adverse events following analyzed, serum sample was...
Background Bronchiolitis caused by respiratory syncytial virus (RSV) is a major cause of mortality and morbidity in infants. Aim To describe RSV epidemiology children the community high-income setting. Methods We used stored blood samples from United Kingdom Born Bradford cohort study that had been collected at birth, age 1 2 years old, tested for IgG postfusion F antibody linked to questionnaires primary hospital care records. finite mixture models classify as infected/not infected...
Neutralizing antibodies are considered a correlate of protection against severe human respiratory syncytial virus (HRSV) disease. Currently, HRSV neutralization assays performed on immortalized cell lines like Vero or A549 cells. It is known that these exclusively detect neutralizing (nAbs) directed to the fusion (F) protein. For detection nAbs glycoprotein (G), ciliated epithelial cells expressing cellular receptor CX3CR1 required, but generation primary cultures expensive and...