A5083735446- Orthopedic Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Infectious Diseases and Tuberculosis
- Osteomyelitis and Bone Disorders Research
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Bacteriophages and microbial interactions
- COVID-19 epidemiological studies
- Streptococcal Infections and Treatments
- COVID-19 Clinical Research Studies
- Infective Endocarditis Diagnosis and Management
- S100 Proteins and Annexins
- Vaccine Coverage and Hesitancy
- Immune Cell Function and Interaction
- Bacterial biofilms and quorum sensing
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Respiratory viral infections research
- Surgical site infection prevention
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
National Institute for Public Health and the Environment
2023-2025
Amsterdam University Medical Centers
2020-2024
University of Amsterdam
2020-2024
AO Foundation
2020-2024
Institute of Neurobiology
2020
As older age and having certain comorbidities can influence humoral responses to vaccination, we studied antibody after the COVID-19 booster campaigns in nursing home (NH) residents. In a two year longitudinal study with Dutch NH residents (n = 107), aged 50 years over, monitored serum prior vaccination third, fourth BNT162b2 (wild-type; WT), bivalent (WT/OMI BA.1) fifth vaccine. Data on vaccinations, infections, comorbidities, and, for some participants, clinical symptoms infection were...
Staphylococcus aureus is a prominent human pathogen in bone and soft-tissue infections. Pathophysiology involves abscess formation, which consists of central staphylococcal communities (SACs), surrounded by fibrin pseudocapsule infiltrating immune cells. Protection against the ingress cells such as neutrophils, or tolerance to antibiotics, remains largely unknown for SACs limited lack availability vitro models. We describe three-dimensional model grown plasma-supplemented collagen gel.
Abstract Cytomegalovirus (CMV) is known to alter circulating effector memory or re-expressing CD45RA + (TemRA) T-cell numbers, but whether Epstein–Barr virus (EBV) does the same this amplified during a CMV and EBV co-infection unclear. Immune cell numbers in blood of children young, middle-aged, senior adults ( n = 336) were determined with flow cytometry, additional multivariate linear regression, intra-group correlation, cluster analyses performed. Compared non-infected controls,...
Abstract Staphylococcus aureus is the most common pathogen that causes implant-associated osteomyelitis, a clinically incurable disease. Immune evasion of S. relies on various mechanisms to survive within bone niche, including secretion leukotoxins such as Panton-Valentine leukocidin (PVL). PVL pore-forming toxin exhibiting selective human tropism for C5a receptors (C5aR1 and C5aR2) CD45 neutrophils, monocytes, macrophages. an important virulence determinant in lung, skin soft tissue...
S. aureus infection of bone is difficult to eradicate due its ability colonize the osteocyte-lacuno-canalicular network (OLCN), rendering it resistant standard-of-care (SOC) antibiotics. To overcome this, we proposed two bone-targeted bisphosphonate-conjugated antibiotics (BCA): sitafloxacin (BCS) and hydroxybisphosphonate-conjugate (HBCS). Initial studies demonstrated that BCA kills in vitro . Here demonstrate vivo efficacy BCS HBCS versus bisphosphonate, sitafloxacin, vancomycin mice with...
A fracture-related infection (FRI) is a serious complication that can occur after surgical fixation of bone fractures. Affected patients may encounter delayed healing and functional limitations. Although it well established Staphylococcus aureus (S. aureus) the main causative pathogen an FRI, pathophysiology S. aureus-induced FRI not characterised over time. Therefore, experimental study in mice comparing aureus-inoculated non-inoculated groups was performed particularly focused on...
Orthopaedic device-related infection (ODRI) presents a significant challenge to the field of orthopaedic and trauma surgery. Despite extensive treatment involving surgical debridement prolonged antibiotic therapy, outcomes remain poor. This is largely due unique abilities Staphylococcus aureus, most common causative agent ODRI, establish protect itself within host by forming biofilms on implanted devices staphylococcal abscess communities (SACs). There need for novel antimicrobials that can...
Staphylococcus aureus is the main causative pathogen of subcutaneous, bone, and implant-related infections, forming structures known as staphylococcal abscess communities (SACs) within tissues that also contain immunosuppressive myeloid-derived suppressor cells (MDSCs). Although both SACs MDSCs are present in chronic S. it remains unknown whether directly trigger MDSC expansion. To investigate this, a previously developed 3D vitro SAC model was co-cultured with murine human bone marrow...