and each was shown to encode a bilirubin transferase isozyme which catalyzes the formation of all physiological conjugates IXa following expression in COS-1 cells.Sequence data showed that cDNAs contained identical 3' ends (1469 base pairs length) other human phenol cDNA, HLUG P1 (Harding, D.,

Multiple administrations of high doses methamphetamine to rats cause long-term depression both dopamine and serotonin synthesis. Coadministration the catecholamine synthesis inhibitor, alpha-methyl-p-tyrosine, antagonizes this effect on neurotransmitter systems. However, when was maintained by administration L-dopa peripheral decarboxylase inhibitor R04-4602, alpha-methyl-p-tyrosine no longer prevented effects either or In addition, specific uptake blocker, amfonelic acid, significantly...

We report the isolation and characterization of two human liver cDNA clones, HUG-Br1 HUG-Br2; each encodes a UDP-glucuronosyltransferase enzyme which glucuronidates bilirubin IX alpha to form both C8 C12 monoconjugates diconjugate. (2351 base pairs) HUG-Br2 (2368 encode proteins with 533 534 amino acid residues, respectively, typical membrane-insertion signal peptide, membrane-spanning domain, 3 or 5 potential asparagine-linked glycosylation sites. At nucleic deduced sequence levels clones...

Uridine-disphosphate glucuronosyl transferase (UGT) enzymes catalyze the formation of glucuronide conjugates phase II metabolism. Methods for absolute quantification UGT1A1 and UGT1A6 were previously established utilizing stable isotope peptide internal standards with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The current method expands upon this by quantifying eight UGT1A isoforms nanobore high-performance chromatography (HPLC) coupled a linear ion trap time-of-flight...

One of the most important mechanisms involved in host defense against xenobiotic chemicals and endogenous toxins is glucuronidation catalysed by UDP-glucuronosyltransferase enzymes (UGT). The role genetic factors determining variable rates not well understood, but phenotypic evidence support such variation has been reported. In present study, six single nucleotide polymorphisms were discovered first exon UGT1A7 gene, which codes for putative substrate-binding domain, revealing a high...

The human cDNA clone, UDPGTh-2, encoding a liver UDP-glucuronosyltransferase (transferase) was isolated from lambda gt11 library by hybridization to the mouse transferase UDPGTm-1 (Kimura, T., and Owens, I. S. (1987) Eur. J. Biochem.168, 515-521). two clones have nucleotide sequence identities in coding region of 74%. UDPGTh-2 encodes 529-amino acid protein with an NH2 terminus membrane-insertion signal peptide carboxyl membrane-spanning region. There are three potential asparagine-linked...

Patients with Crigler-Najjar syndrome (CN) type I inherit an autosomal recessive trait for hyperbilirubinemia, which is characterized by the total absence of bilirubin UDP-glucuronosyltransferase (transferase) activity. The recent identification two transferase isoforms identical carboxyl termini (Ritter, J. K., M. Crawford, and I. S. Owens. 1991. Biol. Chem. 266:1043-1047) led to discovery a unique locus, UGT1, encodes family isozymes, including forms F. Chen, Y. Sheen, H. Tran, Kimura, T....

The human UDP-glucuronosyltransferase 1 (UGT1) locus spans nearly 200 kb on chromosome 2 and encodes nine UGT1A proteins that play a prominent role in drug xenobiotic metabolism. Transgenic UGT1 (Tg-UGT1) mice have been created, it has demonstrated tissue-specific receptor control of the genes is influenced through circulating humoral factors. In Tg-UGT1 mice, are differentially expressed liver gastrointestinal tract. Gene expression profiles confirmed all can be targeted for regulation by...

The <i>UDP-glucuronosyltransferase</i> (<i>UGT</i>) <i>1A</i> genes in humans have been shown to be differentially regulated a tissue-specific fashion. Transgenic mice carrying the human <i>UGT1</i> locus (Tg-<i>UGT1</i>) were recently created, demonstrating that expression of nine <i>UGT1A</i> closely resembles patterns observed tissues. In present study, UGT1A1, UGT1A3, UGT1A4, and UGT1A6 identified as targets peroxisome proliferator-activated receptor (PPAR) α hepatocytes Tg-<i>UGT1</i>...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTwo human liver cDNAs encode UDP-glucuronosyltransferases with 2 log differences in activity toward parallel substrates including hyodeoxycholic acid and certain estrogen derivativesJoseph K. Ritter, Fan Chen, Yhun Y. Sheen, Ronald A. Lubet, Ida S. OwensCite this: Biochemistry 1992, 31, 13, 3409–3414Publication Date (Print):April 7, 1992Publication History Published online1 May 2002Published inissue 7 April...

The NLRP3 inflammasome is activated in the cytoplasm of cells and its products such as IL-1β are exported through a non-classical ER-Golgi pathway. Several mechanistically distinct models including exocytosis secretory lysosomes, microvesicles (MVs) extracellular vehicles (EVs) have been proposed for their release. In this study, we hypothesized that product, response to exogenously administrated endogenously produced d-ribose stimulation released via vesicles EVs sphingolipid-mediated...

The UDP-glucuronosyltransferases (UGTs) have long been known to be inducible by various chemicals, including drugs, although the extent of induction in general has modest. In present study, we determined ability dietary flavonoid chrysin induce UGT activity, protein and mRNA. When pretreating human hepatoma Hep G2 cells with 25 microM chrysin, glucuronidation itself increased 4.2-fold when measured intact cell 14-fold homogenate, i.e., autoinduction. Microsomes from chrysin-treated probed...

Three daily administrations of N-substituted imidazole antimycotics, clotrimazole (CloTZ, 75 mg/kg/day), miconazole (MCZ, 150 or tioconazole (TCZ, but not the 4,5-disubstituted cimetidine (350 mg/kg/day) (200 mg/kg/day for 4 days), induced rat hepatic cytochrome P-450 and other drug-metabolizing enzymes. These findings paralleled in vitro observations where CloTZ, MCZ, TCZ were several orders magnitude more potent as inhibitors p-nitroanisole O-demethylase activity control male liver...

Dysferlin has recently been reported to participate in cell membrane repair muscle and other cells through lysosome fusion. Given that fusion is a crucial mechanism leads raft clustering, the present study attempted determine whether dysferlin involved this process its related signalling, explores underlying dysferlin-mediated bovine coronary arterial endothelial (CAECs). We found clustered macrodomains after Fas Ligand (FasL) stimulation as detected by confocal microscopy fraction...

Medullipin has been proposed to be an antihypertensive lipid hormone released from the renal medulla in response increased arterial pressure and medullary blood flow. Because anandamide (AEA) possesses characteristics of this purported hormone, present study tested hypothesis that AEA or one its metabolites represents medullipin. was demonstrated enriched kidney compared with cortex. Western blotting enzymatic analyses cortical microsomes revealed opposite patterns enrichment two...

The nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) inflammasome has been implicated in podocyte injury and glomerular sclerosis during hyperhomocysteinemia (hHcys). However, it remains unclear whether the NLRP3 can be a therapeutic target for treatment of hHcys-induced kidney injury. Given that DHA metabolites-resolvins have potent anti-inflammatory effects, present study tested prototype, resolvin D1 (RvD1), 17S-hydroxy (17S-HDHA), an intermediate...

Background: Autophagy is of importance in the regulation cell differentiation and senescence podocytes. It possible that derangement autophagy under different pathological conditions activates or enhances Epithelial-to-Mesenchymal Transition (EMT) podocytes, resulting glomerular sclerosis. To test this hypothesis, present study produced lysosome dysfunction by inhibition vacuolar H+-ATPase (V-ATPase) to whether deficiency autophagic flux leads enhancement EMT Methods Results: By Western blot...

The characterization (Ritter, J. K., Chen, F., Sheen, Y. Y., Tran, H. M., Kimura, S., Yeatman, M. T., and Owens, I. S. (1992) Biol. Chem. 267, 3257-3261) of the single-copy UGT1 gene complex encoding both bilirubin phenol UDP-glucuronosyltransferases (transferase) has been critical to determination genetic defects in Crigler-Najjar Type I patients. (UGT1A-UGT1G) codes for at least two bilirubin, three bilirubin-like, transferases. Seven different exons 1, each with an upstream promoter amino...