A5024658133- Inflammasome and immune disorders
- Renal Diseases and Glomerulopathies
- Calcium signaling and nucleotide metabolism
- Ion Transport and Channel Regulation
- Sphingolipid Metabolism and Signaling
- Erythrocyte Function and Pathophysiology
- Autophagy in Disease and Therapy
- Adipokines, Inflammation, and Metabolic Diseases
- Advanced Glycation End Products research
- Adenosine and Purinergic Signaling
- Hormonal Regulation and Hypertension
- Pancreatitis Pathology and Treatment
- Biomedical Research and Pathophysiology
- Eosinophilic Esophagitis
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Eicosanoids and Hypertension Pharmacology
- Hemoglobinopathies and Related Disorders
- Atherosclerosis and Cardiovascular Diseases
- Phagocytosis and Immune Regulation
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Kawasaki Disease and Coronary Complications
- Abdominal vascular conditions and treatments
- Ion channel regulation and function
- Diet and metabolism studies
University of Houston
2016-2025
Pharmac
2020
Virginia Commonwealth University
2010-2019
Virginia Commonwealth University Medical Center
2009-2017
Institute of Pharmacology
2016
Shree Krishna Hospital
2015
University of Richmond
2014
Huazhong University of Science and Technology
2012
University of Tübingen
2004-2011
Czech Academy of Sciences, Institute of Physiology
2009
Plasma trimethylamine-N-oxide (TMAO), a product of intestinal microbial metabolism dietary phosphatidylcholine has been recently associated with atherosclerosis and increased risk cardiovascular diseases (CVD) in rodents humans. However, the molecular mechanisms how TMAO induces CVD progression are still unclear. The present study tested whether NLRP3 inflammasome formation activation thereby contributes to endothelial injury initiating atherogenesis.Inflammasome was determined by confocal...
Inflammasome is a multiprotein complex consisting of Nod-like receptor protein 3 (NALP3), apoptosis-associated speck-like (ASC), and caspase 1 or 5, which functions to switch on the inflammatory process. The present study hypothesized that formation activation NALP3 inflammasomes turn podocyte injury leading glomerulosclerosis during hyperhomocysteinemia (hHcys). RT-PCR Western blot analysis demonstrated murine podocytes expressed essential components inflammasome complex, namely, NALP3,...
Our previous studies have shown that NOD-like receptor protein (NALP3) inflammasome activation is importantly involved in podocyte dysfunction and glomerular sclerosis induced by hyperhomocysteinemia (hHcys). The present study was designed to test whether nicotinamide adenine dinucleotide phosphate (NADPH) oxidase-mediated redox signaling contributes homocysteine (Hcys)-induced of NALP3 inflammasomes, an intracellular inflammatory machinery podocytes vitro vivo.In confocal microscopy...
NADPH oxidase-derived reactive oxygen species (ROS) have been reported to activate NLRP3 inflammasomes resulting in podocyte and glomerular injury during hyperhomocysteinemia (hHcys). However, the mechanism by which inflammasome senses ROS is still unknown podocytes upon hHcys stimulation. The current study explored whether thioredoxin-interacting protein (TXNIP), an endogenous inhibitor of antioxidant thioredoxin sensor, mediates hHcys-induced activation consequent injury. In cultured...
Background/Aims: In addition to their action of lowering blood cholesterol levels, statins modulate biological characteristics and functions arterial myocytes such as viability, proliferation, apoptosis, survival contraction. The present study tested whether simvastatin, a prototype statin, enhances autophagy in coronary (CAMs) thereby exert beneficial effects atherosclerosis. Methods Results: Using flow cytometry, we demonstrated that simvastatin significantly increased the autophagsome...
The serum- and glucocorticoid-inducible kinase 1 (SGK1) is transcriptionally upregulated by mineralocorticoids activated insulin. enhances renal tubular Na+-reabsorption accounts for blood pressure increase following high salt diet in mice made hyperinsulinemic dietary fructose or fat. present study describes the vitro vivo efficacy of a novel SGK1 inhibitor (EMD638683). EMD638683 was tested determination SGK1-dependent phosphorylation NDRG1 (N-Myc downstream-regulated gene 1) human cervical...
Although Nlrp3 inflammasome activation in macrophages has been shown to be critical for the development of atherosclerosis upon atherogenic stimuli, it remains unknown whether activated inflammasomes by other non-atherogenic stimuli induce alterations that may contribute concert with factors atherogenesis. Thus, present study tested hypothesis ATP, which is a classical non-lipid danger stimulus, enhances migration macrophage and increases lipids deposition accelerating foam cell formation....
Inflammasomes serve as an intracellular machinery to initiate inflammatory response various danger signals. The present study tested whether inflammasome centered on nucleotide oligomerization domain-like receptor protein 3 (NLRP3) triggers endothelial adipokine visfatin, a major injurious during obesity. NLRP3 components were abundantly expressed in cultured mouse microvascular cells, including NLRP3, apoptosis-associated speck-like protein, and caspase-1. These molecules could be...
Sodium butyrate (NaBu) is reported to play important roles in a number of chronic diseases. The present work aimed investigate the effect NaBu on angiotensin II (Ang II)-induced cardiac hypertrophy and underlying mechanism vivo vitro models. Sprague Dawley rats were infused with vehicle or Ang (200 ng/kg/min) orally administrated without (1 g/kg/d) for two weeks. Cardiac parameters COX2/PGE2 pathway analysed by real-time PCR, ELISA, immunostaining Western blot. cardiomyocytes H9C2 cells used...
Cigarette smoking is a well-established risk factor for renal dysfunction. Smoking associated with damage bears distinct physiological correlations in conditions such as diabetic nephropathy and obesity-induced glomerulopathy. However, the cellular molecular basis of an association remains poorly understood. High mobility group box 1(HMGB1) highly conserved non-histone chromatin protein that largely contributes to pathogenesis chronic inflammatory autoimmune diseases sepsis, atherosclerosis,...
Background: Recent studies have shown that Nlrp3 inflammasome activation is importantly involved in podocyte dysfunction induced by nicotine. The present study was designed to test whether acid sphingomyelinase (Asm) and ceramide signaling play a role mediating nicotine-induced subsequent damage. Methods Results: Nicotine treatment significantly increased the Asm expression production compared control cells. However, prior with amitriptyline, an inhibitor attenuated production. Confocal...
Obesity is a multi-factorial disorder, which often associated with many other significant diseases such as diabetes, hypertension and cardiovascular diseases, osteoarthritis certain cancers. The management of obesity will therefore require comprehensive range strategies focussing on those existing weight problems also at high risk developing obesity. Hence, prevention during childhood should be considered priority, there persistence to adulthood. This article highlights various preventive...
PDK1 activates a group of kinases, including protein kinase B (PKB)/Akt, p70 ribosomal S6 (S6K), and serum glucocorticoid-induced (SGK), that mediate many the effects insulin as well other agonists. interacts with phosphoinositides through pleckstrin homology (PH) domain. To study role this interaction, we generated knock-in mice expressing mutant incapable binding phosphoinositides. The are significantly small, resistant, hyperinsulinemic. Activation PKB is markedly reduced in result lower...
Abstract Background The aerial parts of Vitis vinifera (common grape or European grape) have been widely used in Ayurveda to treat a variety common and stress related disorders. In the present investigation, seed extract V. was evaluated for antistress activity normal induced rats. Furthermore, studied nootropic rats in-vitro antioxidant potential correlate its activity. Methods For evaluation activity, groups (n = 6) were subjected forced swim one hour after daily treatment extract. Urinary...
The present study tested a hypothesis that excess accumulation of sphingolipid, ceramide, its metabolites, or combination contributes to the development obesity and associated kidney damage. Liquid chromatography/mass spectrometry analysis demonstrated C57BL/6J mice on high-fat diet (HFD) had significantly increased plasma total ceramide levels compared with animals fed low-fat (LFD). Treatment acid sphingomyelinase (ASMase) inhibitor amitriptyline attenuated HFD-induced levels....
The NLRP3 inflammasome has been reported to be activated by atherogenic factors, whereby endothelial injury and consequent atherosclerotic lesions are triggered in the arterial wall. However, mechanisms activating regulating inflammasomes remain poorly understood. present study tested whether acid sphingomyelinase (ASM) ceramide associated membrane raft (MR) signaling platforms contribute activation of during hypercholesterolemia. We found that 7-ketocholesterol (7-Keto) or cholesterol...
The intestinal microbe-derived metabolite trimethylamine N-oxide (TMAO) is implicated in the pathogenesis of cardiovascular diseases (CVDs). molecular mechanisms how TMAO induces atherosclerosis and CVDs' progression are still unclear. In this regard, high-mobility group box protein 1 (HMGB1), an inflammatory mediator, has been reported to disrupt cell-cell junctions, resulting vascular endothelial hyper permeability leading dysfunction. present study tested whether associated dysfunction...
Accelerated suicidal death or eryptosis of infected erythrocytes may delay development parasitemia in malaria. Eryptosis is inhibited by nitric oxide (NO). The present study has been performed to explore, whether inhibition NO synthase L-NAME modifies the course We show here that (>10 µM) increased phosphatidylserine exposure Plasmodium falciparum human erythrocytes, an effect significantly more marked than noninfected erythrocytes. further berghei mice was decreased (from 50% 18%...
Paclitaxel triggers suicidal erythrocyte death or eryptosis, characterized by exposure of phosphatidylserine at the surface and cell shrinkage. Eryptosis infected erythrocytes may delay development parasitemia thus favourably influence course malaria. The present study explored whether paclitaxel influences in vitro parasite growth eryptosis Plasmodium falciparum human vivo survival berghei mice. Phosphatidylserine exposing were identified utilizing annexin V binding volume was estimated...