A5035776297- Asthma and respiratory diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immunotherapy and Immune Responses
- Sarcoidosis and Beryllium Toxicity Research
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Mast cells and histamine
- Immune Response and Inflammation
- Pulmonary Hypertension Research and Treatments
- Allergic Rhinitis and Sensitization
- Inflammasome and immune disorders
- T-cell and B-cell Immunology
- Eosinophilic Esophagitis
- Eosinophilic Disorders and Syndromes
- Pediatric health and respiratory diseases
- NF-κB Signaling Pathways
- Neonatal Respiratory Health Research
- Monoclonal and Polyclonal Antibodies Research
- Immunodeficiency and Autoimmune Disorders
- Coordination Chemistry and Organometallics
- Drug-Induced Adverse Reactions
- interferon and immune responses
- Inflammatory mediators and NSAID effects
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
Erasmus MC
2015-2024
Cancer Genomics Centre
2023
Danone (Netherlands)
2023
Nutricia Research (Netherlands)
2023
Erasmus University Rotterdam
2011-2021
Pulmonary Associates
2008-2020
Ghent University Hospital
2011-2016
Codarts Rotterdam
2016
VIB-UGent Center for Inflammation Research
2014-2016
University of California, San Francisco
2015
Alum (aluminum hydroxide) is the most widely used adjuvant in human vaccines, but mechanism of its adjuvanticity remains unknown. In vitro studies showed no stimulatory effects on dendritic cells (DCs). absence adjuvant, Ag was taken up by lymph node (LN)–resident DCs that acquired soluble via afferent lymphatics, whereas after injection alum, up, processed, and presented inflammatory monocytes migrated from peritoneum, thus becoming induced a persistent Th2 response. The enhancing alum both...
It is unclear how Th2 immunity induced in response to allergens like house dust mite (HDM). Here, we show that HDM inhalation leads the TLR4/MyD88-dependent recruitment of IL-4 competent basophils and eosinophils, inflammatory DCs draining mediastinal nodes. Depletion only partially reduced immunity, depletion eosinophils had no effect on response. Basophils did not take up inhaled antigen, present it T cells, or express antigen presentation machinery, whereas a population FceRI(+) readily...
Adjuvants are vaccine additives that stimulate the immune system without having any specific antigenic effect of itself. In this study we show alum adjuvant induces release IL-1beta from macrophages and dendritic cells is abrogated in lacking various NALP3 inflammasome components. The also required vivo for innate response to OVA alum. early production influx inflammatory into peritoneal cavity strongly reduced NALP3-deficient mice. activation adaptive cellular immunity OVA-alum initiated by...
Although dendritic cells (DCs) play an important role in mediating protection against influenza virus, the precise of lung DC subsets, such as CD11b− and CD11b+ conventional DCs or plasmacytoid (pDCs), different compartments is currently unknown. Early after intranasal infection, tracheal CD11b−CD11chi migrated to mediastinal lymph nodes (MLNs), acquiring co-stimulatory molecules process. This emigration from was followed by accumulation CD11b+CD11chi trachea interstitium. In MLNs, contained...
Pulmonary sarcoidosis is classically defined by T-helper (Th) cell type 1 inflammation (e.g., IFN-γ production CD4(+) effector T cells). Recently, IL-17A-secreting cells have been found in lung lavage, invoking Th17 immunity sarcoidosis. Studies also identified that expressed IFN-γ, but their abundance as a percentage of total was either low or undetermined.Based on evidence can be polarized to Th17.1 produce only our goal determine whether are prominent source sarcoidosis.We developed...
Airway DCs play a crucial role in the pathogenesis of allergic asthma, and interfering with their function could constitute novel form therapy. The sphingosine 1-phosphate receptor agonist FTY720 is an oral immunosuppressant that retains lymphocytes lymph nodes spleen, thus preventing lymphocyte migration to inflammatory sites. accompanying lymphopenia be serious side effect would preclude use as antiasthmatic drug. Here we show murine asthma model local application via inhalation prior or...
Prostaglandins (PGs) can enhance or suppress inflammation by acting on different receptors expressed hematopoietic and nonhematopoietic cells. Prostaglandin D2 binds to the D prostanoid (DP)1 DP2 receptor is seen as a critical mediator of asthma causing vasodilation, bronchoconstriction, inflammatory cell influx. Here we show that inhalation selective DP1 agonist suppresses cardinal features targeting function lung dendritic cells (DCs). In mice treated with receiving agonist-treated DCs,...
It was previously shown that administration of recombinant human Fms-like tyrosine kinase receptor-3 ligand (Flt3L) before allergen challenge sensitized mice suppresses the cardinal features asthma through unclear mechanisms. Here, we show Flt3L dramatically alters balance conventional to plasmacytoid dendritic cells (pDCs) in lung favoring accumulation pDCs. Selective removal pDCs abolished antiinflammatory effect Flt3L, suggesting a regulatory role for these ongoing asthmatic inflammation....
The lung-draining mediastinal lymph nodes (MLNs) are currently widely used to diagnose sarcoidosis. We previously reported that T-helper (Th) 17.1 cells responsible for the exaggerated interferon-γ production in sarcoidosis lungs. In this study, we aimed investigate 1) whether Th17.1 also increased MLNs of patients and 2) frequencies at diagnosis may correlate with disease progression.MLN from treatment-naive pulmonary (n=17) healthy controls (n=22) peripheral blood mononuclear (n=34)...
Abstract Allergic asthma is thought to be regulated by Th2 cells, and inhibiting this reponse a promising mode of intervention. Many studies have focused on differentiation Th cells the Th1 or subset in vitro. IL-4 essential for development, while IL-12 induces which can enhanced IL-18. In present study, we investigated whether IL-18 were able interfere development associated airway symptoms mouse model allergic asthma. Mice sensitized with OVA using protocol that IgE production. Repeated...
Inhalation of iloprost, a stable prostacyclin (PGI(2)) analog, is well-accepted and safe treatment for pulmonary arterial hypertension. Although iloprost mainly acts as vasodilator by binding to the I prostanoid (IP) receptor, recent evidence suggests that signaling via this receptor also has antiinflammatory effects through unclear mechanisms. Here we show in murine model asthma inhalation suppressed cardinal features when given during priming or challenge phase. As mechanism action,...