A5051066209- Immunodeficiency and Autoimmune Disorders
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Chronic Lymphocytic Leukemia Research
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- Cancer Immunotherapy and Biomarkers
- Eosinophilic Esophagitis
- CAR-T cell therapy research
- Immune cells in cancer
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Sarcoidosis and Beryllium Toxicity Research
- Lymphoma Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Pulmonary Hypertension Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Psoriasis: Treatment and Pathogenesis
- Epigenetics and DNA Methylation
- Mast cells and histamine
- Occupational and environmental lung diseases
- Phagocytosis and Immune Regulation
- Diabetes and associated disorders
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
Erasmus MC
2016-2025
Erasmus University Rotterdam
2014-2024
Metropolitan University
2024
Rockefeller University
2023
Erasmus MC Cancer Institute
1994-2022
University Medical Center
2021-2022
University Hospital and Clinics
2021-2022
Pulmonary Associates
2009-2020
Codarts Rotterdam
2020
Leiden University Medical Center
1986-2019
Allergic asthma is characterized by chronic airway inflammation and hyperreactivity thought to be mediated an adaptive T helper‐2 ( h2) cell‐type immune resp‐onse. Here, we demonstrate that type 2 pulmonary innate lymphoid cells ILC 2s) significantly contribute production of the key cytokines IL ‐5 ‐13 in experimental asthma. In naive mice, lineage‐marker negative 2s expressing ‐7Rα, CD 25, S ca‐1, 1/ ST 2(IL‐33R) were present lungs mediastinal lymph nodes M ed LN s), but not...
Tertiary lymphoid organs (TLOs) are organized aggregates of B and T cells formed in postembryonic life response to chronic immune responses infectious agents or self-antigens. Although CD11c+ dendritic (DCs) consistently found regions TLO, their contribution TLO organization has not been studied detail. We that CD11c(hi) DCs essential for the maintenance inducible bronchus-associated tissue (iBALT), a form induced lungs after influenza virus infection. Elimination had cleared from lung...
Abstract Background Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of immature that accumulates in tumour-bearing hosts. These induced by tumour-derived factors (e.g. prostaglandins) and have critical role immune suppression. MDSC suppress T NK cell function via increased expression arginase I production reactive oxygen species (ROS) nitric oxide (NO). Immune suppression was found to be one the main for immunotherapy insufficiency. Here we investigate if vivo...
Pulmonary sarcoidosis is classically defined by T-helper (Th) cell type 1 inflammation (e.g., IFN-γ production CD4(+) effector T cells). Recently, IL-17A-secreting cells have been found in lung lavage, invoking Th17 immunity sarcoidosis. Studies also identified that expressed IFN-γ, but their abundance as a percentage of total was either low or undetermined.Based on evidence can be polarized to Th17.1 produce only our goal determine whether are prominent source sarcoidosis.We developed...
Group 2 innate lymphoid cells (ILC2s; also called nuocytes, helper cells, or natural cells) provide protective immunity during helminth infection and play an important role in influenza-induced allergic airway hyperreactivity. Whereas the transcription factor GATA binding protein 3 ( Gata3 ) is for production of IL-5 -13 by ILC2s response to IL-33 -25 stimulation, it not known whether required ILC2 development from hematopoietic stem cells. Here, we show that chimeric mice generated with...
Group 3 innate lymphoid cells (ILC3) include IL-22–producing NKp46+ and IL-17A/IL-22–producing CD4+ tissue inducerlike that express RORγt are implicated in protective immunity at mucosal surfaces. Whereas the transcription factor Gata3 is essential for T cell ILC2 development from hematopoietic stem (HSCs) IL-5 IL-13 production by ILC2, role generation or function of other ILC subsets not known. We found abundant GATA-3 protein expressed mucosa-associated ILC3 with levels intermediate...
GATA-2 is an essential transcription factor in the hematopoietic system that expressed stem cells (HSCs) and progenitors. Complete deficiency of mouse leads to severe anemia embryonic lethality. The role dosage effects this HSC development within embryo adult are largely unexplored. Here we examined gene on generation expansion HSCs several sites throughout development. We show a haploid dose severely reduces production specifically aorta-gonad-mesonephros region (which autonomously...
Transcription factors act in concert to induce lineage commitment towards Th1, Th2, or T regulatory (Treg) cells, and their counter-regulatory mechanisms were shown be critical for polarization between Th1 Th2 phenotypes. FOXP3 is an essential transcription factor natural, thymus-derived (nTreg) inducible Treg (iTreg) commitment; however, the regulating its expression are as yet unknown. We describe a mechanism controlling iTreg polarization, which overruled by differentiation pathway....
Integrin-mediated adhesion and B cell antigen receptor (BCR) signaling play a critical role in development function, including antigen-specific differentiation. Here we show that the BCR controls integrin alpha4beta1 (VLA-4)-mediated of cells to vascular molecule-1 fibronectin. Molecular dissection underlying mechanism by combined biochemical, pharmacological, genetic approach demonstrates this BCR-controlled integrin-mediated requires (consecutive) activation Lyn, Syk, phosphatidylinositol...
Abstract X‐linked agammaglobulinemia (XLA) is an immunodeficiency disease in man, resulting from arrest early B cell differentiation. The gene defective XLA has recently been identified and encodes a cytoplasmic protein tyrosine kinase, named Bruton's kinase (btk) , essential for differentiation proliferation at the transition pre‐B to later stages. In this study we investigated btk expression by Northern blotting experiments series of human (precursor‐) lines, acute lymphoblastic leukemias...
GATA-3 is a zinc-finger transcription factor that essential for both early T cell development and Th2 differentiation. To quantify expression during in vivo the mouse, gene was targeted by insertion of lacZ reporter homologous recombination embryonic stem (ES) cells. Although we could detect GATA-3+ cells throughout thymus, proportions varied considerably between distinct differentiation stages. The two periods TCR α β recombination, which occur quiescent or slowly dividing cells, were...
X-linked agammaglobulinemia(XLA) is an inherited human immunodeficiency disease, characterized by arrest in B-cell development, which results a dramatic decrease immunoglobulin production.The gene product defective XLA has been identified as cytoplasmic protein tyrosine kinase, named Bruton's kinase (Btk).The M A phenotype indicates critical role for Btk the regulation of development.However, neither external stimuli leading to activation nor any its vivo substrates have thus far identified,...