A5040093820- Renal Diseases and Glomerulopathies
- Renal Transplantation Outcomes and Treatments
- Chronic Kidney Disease and Diabetes
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Cell Adhesion Molecules Research
- Complement system in diseases
- Organ Transplantation Techniques and Outcomes
- Inflammatory mediators and NSAID effects
- Renin-Angiotensin System Studies
- Neurological Complications and Syndromes
- NF-κB Signaling Pathways
- Organ Donation and Transplantation
- Immune Cell Function and Interaction
- Chemokine receptors and signaling
- Reproductive System and Pregnancy
- Eicosanoids and Hypertension Pharmacology
- Systemic Lupus Erythematosus Research
- Hormonal Regulation and Hypertension
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Polyomavirus and related diseases
- Immunotherapy and Immune Responses
- Vasculitis and related conditions
- Nitric Oxide and Endothelin Effects
Universität Hamburg
2013-2023
University Medical Center Hamburg-Eppendorf
2013-2023
Martini-Klinik
2012-2022
Eppendorf (Germany)
1994-2017
Klinik und Poliklinik für Psychosomatische Medizin und Psychotherapie
2009-2017
University of Ottawa
2002
Vanderbilt University
2002
Johannes Gutenberg University Mainz
2001
Emory University
2001
Innsbruck Medical University
2001
Abstract —Incubation of endothelial cells in vitro with high concentrations glucose activates protein kinase C (PKC) and increases nitric oxide synthase (NOS III) gene expression as well superoxide production. The underlying mechanisms remain unknown. To address this issue an vivo model, diabetes was induced streptozotocin rats. Streptozotocin treatment led to dysfunction increased vascular production, assessed by lucigenin- coelenterazine-derived chemiluminescence. bioavailability (as...
Leptin stimulates proliferation and TGF-β expression in renal glomerular endothelial cells: Potential role glomerulosclerosis.BackgroundLeptin inhibits food intake increases energy expenditure. Although the kidney expresses abundant transcripts of short form leptin receptor (Ob-Ra), a for this hormone function remains unclear. Because individuals with massive obesity who may exhibit increased serum concentrations develop glomerulosclerosis, we studied whether can influence growth...
Glomerular influx of monocytes/macrophages (M/M) occurs in many immune- and non-immune-mediated renal diseases. The mechanisms targeting M/M into the glomerulus are incompletely understood, but may involve stimulated expression chemokines. We investigated whether angiotensin II (ANG II) induces chemokine RANTES cultured glomerular endothelial cells rat vivo. ANG mRNA protein cells. II-induced was chemotactic for human monocytes. Surprisingly, II-stimulated transduced by AT2 receptors because...
Regulatory macrophages (M regs) were administered to two living-donor renal transplant recipients. Both patients minimized low-dose tacrolimus monotherapy within 24 wk of transplantation and subsequently maintained excellent graft function. After central venous administration, most M regs remained viable seen traffic from the pulmonary vasculature via blood liver, spleen, bone marrow. By 1 y posttransplantation, both displayed patterns peripheral gene expression converging upon IOT-RISET...
This is a randomized trial (ATHENA study) in de novo kidney transplant patients to compare everolimus versus mycophenolic acid (MPA) with similar tacrolimus exposure both groups, or concomitant cyclosporine (CsA), an unselected population. In this 12-month, multicenter, open-label study, recipients were (EVR/TAC), CsA (EVR/CsA) MPA (MPA/TAC), groups. Non-inferiority of the primary end point (estimated glomerular filtration rate [eGFR] at month 12), assessed per-protocol population 338...
CsA induces a reversible inhibition of proliferation in cultured murine proximal tubular cells (MCT cells) and syngeneic tubulointerstitial fibroblasts (TFB). To test whether this effect may be caused by endogenous synthesis release transforming growth factor-β1 (TGF-β1), well-known inhibitor mitosis, MCT TFB grown serum-free media were treated with different concentrations CsA. CsA, dose-dependent manner range 500–2000 ng/ml, stimulated expression TGF-β1 protein steady-state mRNA levels...
Chemokines and chemokine receptors are implicated in regulatory T cell (Treg) trafficking to sites of inflammation suppression excessive immune responses inflammatory autoimmune diseases; however, the specific requirements for Treg migration into inflamed organs positioning these cells within tissue incompletely understood. Here, we report that Tregs expressing H 1–associated receptor CXCR3 enriched kidneys patients with ANCA–associated crescentic GN colocalize + effector cells. To...
Hypertrophy of mesangial cells is an early hallmark diabetic nephropathy. We have previously shown that murine (MMC), cultured in high-glucose medium, are arrested the G1 phase cell cycle and undergo hypertrophy. This study was undertaken to test whether high glucose-containing medium influences expression p27Kip1, inhibitor active cyclin-dependent kinases (CDK). Incubation MMC, absence other factors for 48-96 h, containing D-glucose (450 mg/dl), stimulated p27Kip1 protein but failed...
Background. Experimental studies suggest that the infiltration of activated T cells into allograft, key event in development acute renal allograft rejection, depends on expression chemokines and their interaction with chemokine receptors expressed cells. Methods. For a more detailed comprehension pathogenesis T-cell recruitment human situ allografts 26 patients between day 3 9 after transplantation was examined present prospective study. Results. Immunohistochemical staining showed...
The recruitment of inflammatory cells into renal tissue, mainly T and monocytes, is a typical feature various diseases such as glomerulonephritis, thrombotic angiopathies, allograft rejection, vasculitis. predominantly infiltrate the tubulointerstitium, whereas monocytes are present in tubulointerstitial glomerular compartment. Because chemokines play pivotal role leukocyte trafficking under conditions, this study investigated whether differential expression contributes to precise...
Angiotensin II (AngII) mediates proinflammatory properties by activating NF-kappaB transcription factor nuclear translocation and inducing the expression of chemokines. For examination whether AngII modulates Toll-like receptor 4 (TLR4), a key element innate immune system that senses LPS, mouse mesangial cells (MMC) were treated with AngII. upregulated TLR4 mRNA protein in MMC, this effect was mediated through type 1 receptors. Reporter gene experiments indicate an protein-1 (AP-1) as well...
Inflammation conveys the development of glomerular injury and is a major cause progressive kidney disease. NF-κB signaling among most important regulators proinflammatory signaling. Its role in podocytes, epithelial cells at filtration barrier, poorly understood. Here, we inhibited podocytes by specific ablation essential modulator (NEMO, IKKγ). Podocyte-specific NEMO-deficient mice (NEMO(pko)) were viable did not show proteinuria or overt changes morphology. After induction...
BackgroundKidney recipients maintaining a prolonged allograft survival in the absence of immunosuppressive drugs and without evidence rejection are supposed to be exceptional. The ERA-EDTA-DESCARTES working group together with Nantes University launched European-wide survey identify new patients, describe them estimate their frequency for first time.
Early diabetic nephropathy is characterized by glomerular hypertrophy. Previous studies in vitro have demonstrated that mesangial cells exposed to high glucose are arrested the G1-phase of cell cycle and express increased levels cyclin-dependent kinase inhibitor p27Kip1. The present study was performed investigate renal expression p27Kip1 db/db mice, a model diabetes mellitus type II. Glomerular protein, but not mRNA expression, strongly enhanced mice compared with non-diabetic db/+...