A5054593137- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Transplantation: Methods and Outcomes
- Acute Kidney Injury Research
- Systemic Sclerosis and Related Diseases
- Neurological Complications and Syndromes
- Complement system in diseases
- Receptor Mechanisms and Signaling
- Renin-Angiotensin System Studies
- Hormonal Regulation and Hypertension
- Monoclonal and Polyclonal Antibodies Research
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Liver Disease and Transplantation
- Renal Diseases and Glomerulopathies
- Systemic Lupus Erythematosus Research
- Dialysis and Renal Disease Management
- Renal and Vascular Pathologies
- Organ Donation and Transplantation
- Atherosclerosis and Cardiovascular Diseases
- Parathyroid Disorders and Treatments
- Mast cells and histamine
- Eicosanoids and Hypertension Pharmacology
- Urticaria and Related Conditions
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Nitric Oxide and Endothelin Effects
Charité - Universitätsmedizin Berlin
2015-2024
Humboldt-Universität zu Berlin
2004-2024
Freie Universität Berlin
1999-2024
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2016-2023
RELX Group (United States)
2022
Berlin-Brandenburger Centrum für Regenerative Therapien
2014-2021
Prevention of Organ Failure
2016
Development Fund
2011-2016
University of Lübeck
2015
University Hospital Schleswig-Holstein
2015
Antibodies against HLA antigens cause refractory allograft rejection with vasculopathy in some, but not all, patients.We studied 33 kidney-transplant recipients who had vascular rejection. Thirteen donor-specific anti-HLA antibodies, whereas 20 did not. Malignant hypertension was present 16 of the patients without 4 whom seizures. The remaining 17 no malignant hypertension. We hypothesized that activating antibodies targeting angiotensin II type 1 (AT1) receptor might be involved.Activating...
Adipose tissue inflammation may play a critical role in the pathogenesis of insulin resistance (IR). The present study examined lymphocytes adipose and IR.In mouse model obesity-mediated IR, high-fat diet (HFD) induced IR already after 5 weeks, which was associated with marked T-lymphocyte infiltration visceral tissue. In contrast, recruitment macrophages delayed an increase MAC3-positive staining F4/80 mRNA expression 10 weeks HFD, suggesting dissociation macrophage invasion into...
The XV. Banff conference for allograft pathology was held in conjunction with the annual meeting of American Society Histocompatibility and Immunogenetics Pittsburgh, PA (USA) focused on refining recent updates to classification, advances from working groups, standardization molecular diagnostics. This report kidney transplant details clarifications refinements criteria chronic active (CA) T cell–mediated rejection (TCMR), borderline, antibody-mediated (ABMR). main focus sessions how address...
The XIII Banff meeting, held in conjunction the Canadian Society of Transplantation Vancouver, Canada, reviewed clinical impact updates C4d-negative antibody-mediated rejection (ABMR) from 2013 reports active Working Groups, relationships donor-specific antibody tests (anti-HLA and non-HLA) with transplant histopathology, questions molecular diagnostics. use transcriptome gene sets, their resultant diagnostic classifiers, or common key genes to supplement diagnosis classification requires...
Objective: To compare the value of novel with conventional serum biomarkers in prediction acute kidney injury (AKI) adult cardiac surgical patients according to preoperative renal function. Design: Single-center, prospective observational study. Setting: Tertiary hospital. Patients: One hundred patients. Measurements and Main Results: We measured concentrations plasma neutrophil gelatinase-associated lipocalin (NGAL), cystatin C, creatinine urea at baseline, on arrival intensive care unit...
Background Systemic sclerosis (SSc) features autoimmunity, vasculopathy and tissue fibrosis. The renin-angiotensin endothelin systems have been implicated in A role for autoantibody-mediated receptor stimulation is hypothesised, linking three major pathophysiological consistent with SSc. Methods Serum samples from 478 patients SSc (298 the study cohort 180 two further independent cohorts), 372 healthy subjects 311 control-disease were tested antibodies against angiotensin II type 1 (AT R)...
Abstract —Hypertension and kidney damage in the double transgenic rat (dTGR) harboring both human renin angiotensinogen genes are dependent on components of angiotensin system. We tested hypothesis that monocyte infiltration increased adhesion molecule expression involved pathogenesis dTGR. also evaluated effects long-term angiotensin-converting enzyme (ACE) inhibition, AT 1 blockade, inhibition recruitment inflammatory response Systolic blood pressure 24-hour albuminuria were markedly...
Acute kidney injury (AKI) after cardiac surgery is a common and serious condition carrying significant costs. During cardiopulmonary bypass (CPB) surgery, modifiable factors may contribute to post-operative AKI. Their avoidance might be potential target for nephroprotection. The objective of the present study was identify determine whether intraoperative hypotension, anaemia, or their combination, red blood cell transfusion vasopressor use are independent risk AKI defined by RIFLE (renal...
Objective: To test whether perioperative sodium bicarbonate infusion can attenuate postoperative increases in serum creatinine cardiac surgical patients. Design: Double-blind, randomized controlled trial. Setting: Operating rooms and intensive care unit at a tertiary hospital. Patients: Cohort of 100 patients increased risk acute renal dysfunction. Intervention: Patients were to either 24 hrs intravenous (4 mmol/kg) or chloride mmol/kg). Measurements Main Results: The primary outcome measure...
Background. Angiotensin type 1 receptor (AT1R) mediates most physiologic and pathophysiologic actions of its endogenous ligand, angiotensin II, with overactivity leading to vascular remodeling hypertension. Antibodies AT1R are implicated in several pathologies. The aim our study was determine the impact antibody on clinical outcomes including mediated rejection (AMR), or without C4d deposition, patients whose sera contained no donor human leukocyte antigen (HLA)-specific (HLA-DSA). Methods....
The angiotensin II type 1 receptor (AT1R) is an emerging target of functional non-HLA antibodies (Ab). We examined the potential determining degree presensitization against AT1R as a risk factor for graft survival and acute rejection (AR). study included 599 kidney recipients between 1998 2007. Serum samples were analyzed in blinded fashion anti-AT1R (AT1R-Abs) using quantitative solid-phase assay. A threshold AT1R-Ab levels was statistically determined at 10 U based on time to failure. An...
Rationale: Systemic sclerosis (SSc)-associated pulmonary arterial hypertension (PAH) portends worse outcome than other forms of PAH. Vasoconstrictive and vascular remodeling actions endothelin (ET) 1 angiotensin (Ang) II via receptor type A (ETAR) Ang type-1 (AT1R) activation are implicated in PAH pathogenesis.Objectives: We hypothesized that stimulating autoantibodies (Abs) targeting activating AT1R ETAR may contribute to SSc-PAH pathogenesis, tested their functional biomarker...
Background. In adult cardiac surgery, the predictive value for AKI of neutrophil gelatinase-associated lipocalin (NGAL) appears to have wide variability. The choice definition acute kidney injury (AKI) might, at least in part, account such Methods. a prospective study 100 surgery patients, we assessed postoperative plasma NGAL predicting according degree severity used its definition. Results. varied and was higher more severe (increase creatinine >50%: mean AUC–ROC 0.79 ± 0.01) compared less...
Autoantibodies have been associated with autoimmune diseases. However, studies identified autoantibodies in healthy donors (HD) who do not develop disorders. Here we provide evidence of a network immunoglobulin G (IgG) targeting protein-coupled receptors (GPCR) HD compared to patients systemic sclerosis, Alzheimer's disease, and ovarian cancer. Sex, age pathological conditions affect autoantibody correlation hierarchical clustering signatures, yet many the correlations are shared across all...
Vasculopathy, inflammatory fibrosis and functional autoantibodies (Abs) are major manifestations of systemic sclerosis (SSc). Abs directed against the angiotensin II type 1 receptor (AT₁R) endothelin-1 A (ETAR) associated with characteristic disease features including vascular, inflammatory, fibrotic complications indicating their role in SSc pathogenesis. Therefore, impact anti-AT₁R anti-ETAR on initiation inflammation was analyzed.Anti-AT₁R Ab-positive immunoglobulin G (IgG) from patients...
Non-human leukocyte antigen antibodies (Abs) targeting vascular receptors are implicated in the pathogenesis of renal allograft rejection and progressive vasculopathy patients with systemic sclerosis.We prospectively tested 30 heart transplant recipients impact Abs directed against endothelin-1 type A (ET(A)R) angiotensin II 1 (AT(1)R, cell-enzyme-linked immunosorbent assay) at time transplantation during first posttransplantation year on cellular Ab-mediated (immunohistochemistry, C3d,...
Abstract Introduction Agonistic autoantibodies (Aabs) against the angiotensin II receptor type 1 (AT1R) and endothelin A (ETAR) have been identified in patients with systemic sclerosis (SSc). In our present study, we examined expression of AT1R ETAR human immune cells pathological effects mediated through these receptors by their corresponding Aabs. Methods Protein on peripheral blood mononuclear (PBMCs) from healthy individuals SSc was analyzed using flow cytometry, mRNA both PBMCs donors...
While therapeutic mesenchymal stromal/stem cells (MSCs) have usually been obtained from bone marrow, perinatal tissues emerged as promising new sources of for stromal cell therapy. In this study, we present a first safety follow-up on our clinical experience with placenta-derived decidual (DSCs), used supportive immunomodulatory and regenerative therapy patients severe complications after allogeneic hematopoietic stem transplantation (HSCT). We found that DSCs are smaller, almost half the...