A5077141230- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Renal Diseases and Glomerulopathies
- Pancreatic function and diabetes
- Transplantation: Methods and Outcomes
- Renal and Vascular Pathologies
- Organ Donation and Transplantation
- Xenotransplantation and immune response
- Organ and Tissue Transplantation Research
- Polyomavirus and related diseases
- Immunotherapy and Immune Responses
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Animal Virus Infections Studies
- Cannabis and Cannabinoid Research
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Renal cell carcinoma treatment
- Tissue Engineering and Regenerative Medicine
- Lipoproteins and Cardiovascular Health
- Cerebrospinal fluid and hydrocephalus
- Neurological Complications and Syndromes
- Pancreatic and Hepatic Oncology Research
- Diabetes Management and Research
- RNA Research and Splicing
- Fetal and Pediatric Neurological Disorders
Leiden University Medical Center
2010-2020
Leiden University
2011-2018
University of Amsterdam
2008-2018
Amsterdam UMC Location University of Amsterdam
2008-2016
Hammersmith Hospital
2012-2015
Imperial College Healthcare NHS Trust
2012-2015
Centre for Inflammation Research
2015
Imperial College London
2015
Maastricht University Medical Centre
2006
The XIII Banff meeting, held in conjunction the Canadian Society of Transplantation Vancouver, Canada, reviewed clinical impact updates C4d-negative antibody-mediated rejection (ABMR) from 2013 reports active Working Groups, relationships donor-specific antibody tests (anti-HLA and non-HLA) with transplant histopathology, questions molecular diagnostics. use transcriptome gene sets, their resultant diagnostic classifiers, or common key genes to supplement diagnosis classification requires...
In renal transplant patients with de novo donor-specific antibodies (dnDSA) we studied the value of microcirculation inflammation (MI; defined by addition glomerulitis (g) and peritubular capillaritis (ptc) scores) to assess long-term graft survival in a retrospective cohort study. Out all standard immunological risk (n = 638), 79 (12.4%) developed dnDSA 58/79 (73%) had an indication biopsy at or after development. Based on MI score that were categorized, MI0 26), MI1 + 2 21) ≥ 3 11). The...
C4d+ antibody-mediated rejection following pancreas transplantation has not been well characterized. Therefore, we assessed the outcomes of 27 patients (28 biopsies), with both C4d staining and donor-specific antibodies (DSA) determined, from a cohort 257 patients. The median follow-up was 50 (interquartile range [IQR] 8-118) months. Patients were categorized into 3 groups: group 1, minimal or no DSA (n = 13); 2, either present but C4d, diffuse focal 6); 3, 9). Active septal inflammation,...
Chronic antibody-mediated rejection is an important cause of late graft failure. Developing early marker the disease may allow diagnosis and treatment before irreversible damage has occurred. The aim this study was to assess whether, on electron microscopy examination, peritubular capillary (PTC) basement membrane multilayering precedes predicts development transplant glomerulopathy (TG).We used a vintage matched case-control method. Sixteen pairs were created among all renal patients from...
Early pancreas graft loss is usually attributed to technical failure while the possibility of antibody-mediated rejection (AMR) generally overlooked. To investigate role AMR in early loss, we retrospectively assessed 256 patients with simultaneous pancreas-kidney transplantation (SPK) between 1985 and 2010 at our institute. We included 33 SPK who lost their <1 year after transplantation. was diagnosed based on donor-specific antibodies, C4d histology 7 cases, 8 cases were suspicious for 18...
Microarray studies have shown elevated transcript levels of endothelial and natural killer (NK) cell-associated genes during antibody-mediated rejection (AMR) the renal allograft. This study aimed to assess use quantitative real-time polymerase chain reaction as an alternative microarray analysis on a subset these genes.Thirty-nine transplant biopsies from patients with de novo donor-specific antibodies eighteen 1-year surveillance no histological evidence were analyzed for expression 11...
Diffuse C4d staining in peritubular capillaries (PTCs) during an acute rejection episode (ARE) is the footprint of antibody-mediated rejection. In current clinical practice, diffuse C4d+ regarded as inferior prognostic sign. This case-control study investigated role mere for graft outcome ARE a well defined cohort similarly ARE-treated patients.All kidney transplant recipients authors' center from January 1, 1995 to December 31, 2005 were reviewed. From these patients, 151 had ARE. Paraffin...
Severe peritubular capillary basement membrane multilayering (PTCBML) is part of the Banff definition chronic antibody-mediated rejection. We retrospectively investigated whether assessment mean number layers (BM) around capillaries (PTC) can be used in a cohort patients with de novo donor-specific antibodies (dnDSA) as an early marker to predict long-term injury.This retrospective study 151 electron microscopy samples from 54 dnDSA, assessed at 1 year after transplantation, for BM PTC and...
Allogeneic islets of Langerhans transplantation is hampered in its success as a curative treatment type 1 diabetes by the absence potent, specific, and nontoxic immunosuppressive drugs. Here, we assessed whether donor bone marrow-derived dexamethasone-treated dendritic cells (dexDCs) could prolong islet allograft survival full major histocompatibility complex mismatch rat model.Rodent allogeneic was performed from DA rats to Lewis vice versa. Permanently immature were generated marrow with...
BK polyomavirus (BKV)-associated nephropathy is a threat to kidney allograft survival affecting up 15% of renal transplant patients. Previous studies revealed that tubular epithelial cells (TEC) show limited response towards BKV infection. Here we investigated the interplay between and TEC in more detail. In particular, questioned whether suppresses and/or evades antiviral responses.Human primary peripheral blood mononuclear were infected with Dunlop strain or other viruses. Moreover,...
Pancreas transplant longevity is limited by immune rejection, which diagnosed graft biopsy using the Banff Classification. The histological criteria for antibody-mediated rejection (AMR) are poorly reproducible and inconsistently associated with outcome. We hypothesized that a 34-gene set in other solid organ transplants could improve diagnosis pancreas grafts. AMR set, comprising endothelial, natural killer cell inflammatory genes, was quantified NanoString platform 52 formalin-fixed,...
Introduction Accurate diagnosis of pancreas rejection is required for early, targeted treatment and function preservation. Pancreas biopsy considered the gold standard presents with sampling issues perceived procedural risks that limit its widespread use. Evaluation graft duodenal cuff, through upper endoscopy in intestine drained transplants has been proposed as a surrogate but utility this approach not tested. In multicenter effort we retrospectively evaluated tissues collected...
Viral infections can hamper allograft survival and for kidney transplantation specifically, the double-stranded DNA (dsDNA) BK polyoma virus is a huge threat. In transplant recipients with infection, viral particles be found in urine renal tubular epithelium. Microarray analysis of infection epithelial cells showed lack transcriptional regulation immune mediators (Abend et al Virology 2010;397). We set out to establish which dsDNA sensors are expressed resting stimulated cells. By that, we...
Since 2006, approximately 1.5% of all live donor renal transplantations are ABO incompatible (ABOi) in the USA. In-depth, longitudinal studies on histology this group transplant recipients few number. We retrospectively assessed 164 biopsies (122 indication, 30 surveillance, 12 unknown) taken from 57 ABOi patients at our centre, which has 1, 3 and 5 yr censored allograft survival 98.6, 96.9 90.7% respectively. All were Banff ‘09 classified. Median time to biopsy was 12.5 months (range...