A5089422245- Renal Transplantation Outcomes and Treatments
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Organ Transplantation Techniques and Outcomes
- Cytomegalovirus and herpesvirus research
- Transplantation: Methods and Outcomes
- Organ Donation and Transplantation
- Neurological Complications and Syndromes
- Pharmacological Effects and Toxicity Studies
- Adenosine and Purinergic Signaling
- Blood groups and transfusion
- Gut microbiota and health
- Xenotransplantation and immune response
- Metabolomics and Mass Spectrometry Studies
- Cardiomyopathy and Myosin Studies
- Advanced Fluorescence Microscopy Techniques
- Tryptophan and brain disorders
- Clostridium difficile and Clostridium perfringens research
- Reproductive System and Pregnancy
- Multiple Sclerosis Research Studies
- Bipolar Disorder and Treatment
- Herpesvirus Infections and Treatments
- Polyomavirus and related diseases
- Renal Diseases and Glomerulopathies
- Pregnancy and Medication Impact
Inserm
2016-2023
Center for Research in Transplantation and Translational Immunology
2018-2023
Nantes Université
2017-2023
Centre d'Investigation Clinique de Nantes
2016-2022
Centre Hospitalier Universitaire de Nantes
2013-2022
Institut de Transplantation Urologie en Nephrologie
2015-2022
Laboratoire de Microbiologie et Génétique Moléculaires
2021
RELX Group (United States)
2018
Institut National de Recherche en Santé Publique
2015
Whereas a B cell-transcriptional profile has been recorded for operationally tolerant kidney graft patients, the role that cells have in this tolerance not reported. In study, we analyzed of from healthy volunteers, and transplant recipients with stable function on T cell suppression. Proliferation, apoptosis, type I proinflammatory cytokine production by effector CD4(+)CD25(-) were measured after anti-CD3/anti-CD28 stimulation or without autologous cells. We report inhibit response...
The role of Foxp3 + regulatory T cells (Tregs) in operational tolerance remains elusive, as initial results revealed an increased frequency this subset tolerant patients but no functional differences compared with immunosuppressed recipients. In addition, recent studies B strongly suggest that Tregs may not have a central kidney transplantation tolerance. However, investigations the crucial demethylation Treg function and possibility identifying distinct cell subsets prompted us to more...
Long-term renal transplant outcome is limited by side effects of immunosuppressive drugs, particularly calcineurin inhibitor (CNI). We assumed that some patients selected for a "low immunological risk rejection" could be eligible and benefit from CNI weaning strategy. designed prospective, randomized, multicenter, double-blind placebo-controlled clinical study (Eudract: 2010-019574-33) to analyze the benefit-risk ratio tacrolimus on highly (≥4 years transplantation, normal histology, stable...
Significance Statement Identifying biomarkers for predicting kidney transplant failure requires better understanding of the immune response to chronic allogeneic stimulation. The authors demonstrated that 1 year after transplantation, composition CD8 + memory T cell subsets in blood—specifically ratio terminally differentiated effector (TEMRA) and cells—is associated with risk subsequent graft adds predictive value a previously reported eight-variable clinical score. They also found TEMRA...
ObjectivesMany transplant physicians screen for and treat asymptomatic bacteriuria (ASB) during post-kidney-transplant surveillance. We investigated whether antibiotics are effective in reducing the occurrence of symptomatic urinary tract infection (UTI) kidney recipients with ASB.MethodsWe performed this multicentre, randomized, open-label trial who had ASB were ≥2 months post-transplantation. randomly assigned participants to receive or no therapy. The primary outcome was incidence UTI...
Renal operationally tolerant patients (TOL) display a defect in B cell differentiation, with deficiency plasma cells. Recently described, T follicular helper (Tfh) cells play critical role differentiation. We analyzed blood Tfh subsets TOL and transplanted stable graft function under immunosuppression (STA). observed reduced proportion of activated highly functional TOL, without affecting absolute numbers. Functionally, from displayed modified gene expression profile, failed to produce...
BackgroundKidney recipients maintaining a prolonged allograft survival in the absence of immunosuppressive drugs and without evidence rejection are supposed to be exceptional. The ERA-EDTA-DESCARTES working group together with Nantes University launched European-wide survey identify new patients, describe them estimate their frequency for first time.
Rabbit-generated antithymocyte globulins (ATGs), which target human T cells, are widely used as immunosuppressive agents during treatment of kidney allograft recipients. However, ATGs can induce immune complex diseases, including serum sickness disease (SSD). Rabbit and IgGs have various antigenic differences, expression the sialic acid Neu5Gc α-1-3-Gal (Gal), not synthesized by beings. Moreover, anti-Neu5Gc antibodies been shown to preexist be elicited immunization in subjects. This study...
The mechanisms regulating CD8
Immune response against human cytomegalovirus (HCMV) includes a set of persistent cytotoxic NK and CD8 T cells devoted to eliminate infected prevent reactivation. HCMV antigens (pp65, IE1) presented by HLA class-I molecules are well characterized they associate with efficient virus control. HLA-E-restricted targeting UL40 signal peptides (HLA-EUL40) have recently emerged as non-conventional T-cell also observed in some hosts. The occurrence, specificity features HLA-EUL40 responses remain...
The etiology of multiple sclerosis (MS) remains elusive. Among the possible causes, increase anti-Neu5Gc antibodies during EBV primo-infection Infectious mononucleosis (IMN) may damage integrity blood-brain barrier facilitating transfer EBV-infected B cells and anti-EBV T cell clones in brain. We investigated change titers anti-α1,3 Galactose 49 IMN, 76 MS, 73 clinically isolated syndrome (CIS) patients, as well age/gender-matched healthy individuals. Anti-Gal are significantly increased IMN...
Host-microbiota interactions can modulate the immune system both at local and systemic levels, with potential consequences for organ transplantation outcomes. In this study, we hypothesized that differences in urinary microbiome following kidney would be associated posttransplantation status: stable, minimally immunosuppressed, or tolerant. One hundred thirteen urine samples from stable (n = 51), immunosuppressed 19), spontaneously tolerant 16) patients, paired age-matched controls 27) were...
The involvement of TEMRA CD8 is evident in a large array immunological conditions ranging from auto- to allo-immunity. Nevertheless, the factors leading their accumulation and activation remain ill-defined efficient therapeutics control inflammatory response lacking. Here, we show that IL-15-stimulated kidney transplant recipients promote inflammation by inducing expression CX3CL1 endothelial cells an IFN- TNF- dependent manner. responsiveness IL-15 not restricted chronic stimulation as...
BackgroundOperational tolerance is the holy grail in solid organ transplantation. Previous reports showed that urinary compartment of operationally tolerant recipients harbor a specific and unique profile. We hypothesized spontaneous kidney transplanted (KTR) would have metabolomic profile associated to operational tolerance.MethodsWe performed profiling on urine samples from healthy volunteers, stable KTR under standard minimal immunosuppression using liquid chromatography tandem with mass...
The role of natural killer (NK) cells in organ transplantation is controversial. This study aims to decipher their kidney transplant tolerance humans. Previous studies highlighted several modulated genes involved NK cell biology blood from spontaneously operationally tolerant patients (drug-free kidney-transplanted recipients with stable graft function). We performed a phenotypic, functional, and genetic characterization these compared function under immunosuppression healthy volunteers....
Duchenne muscular dystrophy (DMD) is a neuromuscular disease caused by mutations in the gene encoding dystrophin. It leads to repeated cycles of muscle fiber necrosis and regeneration progressive replacement fibers fibrotic adipose tissue, with consequent weakness premature death. Fibrosis and, particular, collagen accumulation are important pathological features dystrophic muscle. A better understanding development fibrosis crucial enable management DMD. Three-dimensional (3D)...
The human cytomegalovirus (HCMV) triggers both innate and adaptive immune responses, including protective CD8+ αβT cells (CD8T) that contributes to the control of infection. In addition CD8T restricted by classical HLA class Ia molecules, HCMV also recognizing peptides from UL40 leader peptide HLA-E molecules (HLA-EUL40 CD8T). This study investigated frequency, phenotype functions HLA-EUL40 in comparison immunodominant HLA-A2pp65 upon acute (primary or secondary infection) chronic infection...