A5064767427- Xenotransplantation and immune response
- Monoclonal and Polyclonal Antibodies Research
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Viral gastroenteritis research and epidemiology
- Glycosylation and Glycoproteins Research
- Renal Transplantation Outcomes and Treatments
- Extracellular vesicles in disease
- Virus-based gene therapy research
- MicroRNA in disease regulation
- SARS-CoV-2 and COVID-19 Research
- Immune cells in cancer
- Phagocytosis and Immune Regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Diabetes and associated disorders
- Peripheral Neuropathies and Disorders
- Escherichia coli research studies
- Blood groups and transfusion
- Cardiovascular Disease and Adiposity
- Complement system in diseases
- Vasculitis and related conditions
- Systemic Lupus Erythematosus Research
- Multiple Sclerosis Research Studies
- Dermatology and Skin Diseases
Inserm
2014-2025
Nantes Université
2014-2025
Oniris
2010-2024
Center for Research in Transplantation and Translational Immunology
2015-2023
Institut de Transplantation Urologie en Nephrologie
2015-2023
Centre National de la Recherche Scientifique
2022
Centre d'Investigation Clinique de Nantes
2015-2020
John Wiley & Sons (United States)
2019
Hudson Institute
2019
Université Bretagne Loire
2018
In addition to important regulatory roles in gene expression through RNA interference, it has recently been shown that microRNAs display immune stimulatory effects direct interaction with receptors of innate immunity the Toll-like receptor family, aggravating neuronal damage and tumour growth. Yet no evidence exists on consequences microRNA actions context an autoimmune disease. Using analogues, we here show pancreatic beta cell-derived sequences induce pro-inflammatory (TNFa, IFNa, IL-12,...
Colony stimulating factor-1 receptor (CSF-1R) and its ligands CSF-1 interleukin (IL)-34 have tumorigenic effects through both induction of suppressive macrophages, survival/proliferation tumor cells. In addition, the IL-34 effect can also be mediated by other receptors, protein-tyrosine phosphatase zeta, Syndecan-1 (CD138) triggering expressed on myeloid cells 2. Small tyrosine kinase inhibitors are used to block CSF-1R signaling but lack specificity. Neutralizing anti-CSF-1 and/or...
Rabbit-generated antithymocyte globulins (ATGs), which target human T cells, are widely used as immunosuppressive agents during treatment of kidney allograft recipients. However, ATGs can induce immune complex diseases, including serum sickness disease (SSD). Rabbit and IgGs have various antigenic differences, expression the sialic acid Neu5Gc α-1-3-Gal (Gal), not synthesized by beings. Moreover, anti-Neu5Gc antibodies been shown to preexist be elicited immunization in subjects. This study...
Heterologous polyclonal antibodies might represent an alternative to the use of convalescent plasma or monoclonal (mAbs) in coronavirus disease (COVID-19) by targeting multiple antigen epitopes. However, heterologous trigger human natural xenogeneic antibody responses particularly directed against animal-type carbohydrates, mainly N-glycolyl form neuraminic acid (Neu5Gc) and α1,3-galactose, potentially leading serum sickness allergy. Here, we immunized cytidine...
Xenocell therapy from neonate or adult pig pancreatic islets is one of the most promising alternatives to allograft in type 1 diabetes for addressing organ shortage. In humans, however, natural and elicited antibodies specific xenoantigens, α-(1,3)-galactose (GAL) N-glycolylneuraminic acid (Neu5Gc), are likely significantly contribute xenoislet rejection. We obtained double-knockout (DKO) pigs lacking GAL Neu5Gc. Because Neu5Gc−/− mice exhibit glycemic dysregulations β-cell dysfunctions, we...
Polyclonal antihuman thymocyte rabbit IgGs (antithymocyte globulin [ATG]) are popular immunosuppressive drugs used to prevent or treat organ bone-marrow allograft rejection, graft versus host disease, and autoimmune diseases. However, animal-derived glycoproteins also strongly immunogenic ATG induces serum sickness disease in almost all patients without additional drugs, as seen the Study of Thymoglobulin arrest Type 1 Diabetes (START) trial therapy new-onset type diabetes.Using...
Cytokines are major players regulating immune responses towards inflammatory vs. tolerogenic results. In organ and bone marrow transplantation new reagents needed to inhibit tissue destructive mechanisms eventually induce tolerance without overall immunosuppression. IL-34 is a cytokine with no significant homology any other but that acts preferentially through CSF-1R, as CSF-1 does, PTPz. Although share actions, detailed analysis of their effects on cells needs further research. We...
The etiology of multiple sclerosis (MS) remains elusive. Among the possible causes, increase anti-Neu5Gc antibodies during EBV primo-infection Infectious mononucleosis (IMN) may damage integrity blood-brain barrier facilitating transfer EBV-infected B cells and anti-EBV T cell clones in brain. We investigated change titers anti-α1,3 Galactose 49 IMN, 76 MS, 73 clinically isolated syndrome (CIS) patients, as well age/gender-matched healthy individuals. Anti-Gal are significantly increased IMN...
The bioartificial pancreas encapsulating pancreatic islets in immunoprotective hydrogel is a promising therapy for Type 1 diabetes. As are highly metabolically active and exquisitely sensitive to hypoxia, maintaining O2 supply after transplantation remains major challenge. In this study, we address the limitation by combining silicone-encapsulated CaO2 (silicone-CaO2 ) generate with an extracellular hemoglobin -carrier coencapsulated islets. We showed that improved 37% -diffusivity through...
Small extracellular vesicles (sEV) released by healthy beta cells are promising candidates for diabetes therapy thanks to their aptitude modulate inflammation, induce or maintain pancreatic function and prevent pathogenic mechanisms. To advance the clinical development of therapeutics, there is a crucial need scalable production methods. Stirred tank bioreactors (STR) widely used in industry due ability provide homogeneous gas nutrient supply, online monitoring, efficient scale up....
Polyclonal xenogenic IgGs, although having been used in the prevention and cure of severe infectious diseases, are highly immunogenic, which may restrict their usage new applications such as Ebola hemorrhagic fever. IgG glycans display powerful xenogeneic antigens humans, for example α1-3 Galactose glycolyl form neuraminic acid Neu5Gc, IgGs deprived these key sugar epitopes represent an advantage passive immunotherapy. In this paper, we explored whether low immunogenicity had a protective...
Antibodies of non-human mammals are glycosylated with carbohydrate antigens, such as galactose-α-1-3-galactose (α-Gal) and N-glycolylneuraminic acid (Neu5Gc). These antigens highly immunogenic in humans due to loss-of-function mutations the key genes involved their synthesis. Such carbohydrates expressed on therapeutic polyclonal rabbit anti-human T-cell IgGs (anti-thymocyte globulin; ATG), most popular induction treatment allograft recipients. To decipher quantitative qualitative response...
For the first time, N-glycosylation patterns of immunoglobulin G (IgGs) isolated from serum two varieties knockout pigs (lacking N-glycolylneuraminic acid (Neu5Gc) and/or α 1,3 galactose) were examined for presence potential glycan xenoantigens and compared to obtained wild-type (WT) pig IgGs. Glycopeptide analysis was chosen over release, as protein-A eluates may contain IgA IgM shown previously. The experiments focused on tryptic glycopeptides EEQFNSTYR AEQFNSTYR IgGs, excluded IgM, in...
Abstract Perfusion of convalescent plasma (CP) has demonstrated a potential to improve the pneumonia induced by SARS-CoV-2, but procurement and standardization CP are barriers its wide usage. Many monoclonal antibodies (mAbs) have been developed appear insufficient neutralize SARS-CoV-2 unless two or three them being combined. Therefore, heterologous polyclonal animal origin, that used for decades fight against infectious agents might represent highly efficient alternative use mAbs in...
Anti-thymocyte or anti-lymphocyte globulins (ATGs/ALGs) are immunosuppressive drugs used in induction therapies to prevent acute rejection solid organ transplantation. Because animal-derived, ATGs/ALGs contain highly immunogenic carbohydrate xenoantigens eliciting antibodies that associated with subclinical inflammatory events, possibly impacting long-term graft survival. Their strong and long-lasting lymphodepleting activity also increases the risk for infections. We investigated here vitro...
ABSTRACT Anti-thymocyte/lymphocyte globulins (ATGs/ALGs) are immunosuppressive drugs used in induction therapies to prevent acute rejection solid organ transplantation. Because of animal origin, ATGs/ALGs contain highly immunogenic carbohydrate xenoantigens eliciting antibodies that associated with subclinical inflammatory events possibly impacting long-term graft survival. Their strong and long-lasting lymphodepleting activity also increases the risk for infections. To circumvent these...
is associated with significant morbidity and mortality. Studies on a possible association between the occurrence of thrombosis thrombophilia in patients this disease have been controversial. The objective study was to assess frequency clinical relevance anticardiolipin antibodies (aCL) & other thrombophilic factors their relationship