A5018882725- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Immune Cell Function and Interaction
- Galectins and Cancer Biology
- CAR-T cell therapy research
- Infant Nutrition and Health
- Phagocytosis and Immune Regulation
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Proteoglycans and glycosaminoglycans research
- Cancer Mechanisms and Therapy
- Biochemical and Molecular Research
- Immune Response and Inflammation
- Respiratory viral infections research
- Streptococcal Infections and Treatments
- Topic Modeling
- Microbial infections and disease research
- Cancer Immunotherapy and Biomarkers
- Immune cells in cancer
- Chemical Synthesis and Analysis
- Xenotransplantation and immune response
- Natural Language Processing Techniques
University of California, Davis
2016-2025
Commonwealth Scientific and Industrial Research Organisation
2022-2025
Shanghai Veterinary Research Institute
2012-2025
Chinese Academy of Agricultural Sciences
2012-2025
Yangzhou University
2022-2025
Lanzhou Petrochemical Polytechnic
2024
Chungbuk National University
2024
First Affiliated Hospital of Jinan University
2024
Zhejiang Normal University
2023-2024
Meharry Medical College
2024
A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained is (1) an α2,3-sialyltransferase (main function), (2) α2,6-sialyltransferase, (3) α2,3-sialidase, (4) α2,3-trans-sialidase. The tPm0188Ph powerful tool synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high...
Glucans are structurally diverse fungal biopolymers that stimulate innate immunity and pathogen-associated molecular patterns. Dectin-1 is a C-type lectin-like pattern recognition receptor binds glucans induces immune responses to pathogens. We examined the effect of glucan structure on binding by murine recombinant with library natural product synthetic (1→3)-β/(1→6)-β-glucans as well nonglucan polymers. highly specific for pure (1→3)-β-linked backbone structure. Although (1→3)-β-d-glucans,...
Just relax: A one-pot, three-enzyme chemoenzymatic synthetic approach is highly efficient for obtaining complex sialosides that contain diverse naturally occurring sialic acid modifications. α-2,6-Linked containing twelve forms and seven non-natural have been obtained. Supporting information this article available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z600572_s.pdf or from author. Please note: The publisher not responsible content functionality of any supporting...
A comparison of urinary alpha 1-microglobulin concentrations to the behaviour other indicators renal tubular disorders, beta 2-microglobulin, retinol-binding protein and N-acetyl-beta-D-glucosaminidase (NAG) has been made. In acute disorders 2M RBP are highly correlated (r = 0.89) but this is less marked for 1M 0.55) r 0.48. NAG tends run a parallel course lags behind recovery low molecular weight reabsorption following injury cells. The 1M, in particular its stability at pH suggest that may...
The nonhuman sialic acid N-glycolylneuraminic (Neu5Gc) is metabolically incorporated into human tissues from certain mammalian-derived foods, and this occurs in the face of an anti-Neu5Gc “xeno-autoantibody” response. Given evidence that process contributes to chronic inflammation some diseases, it important understand when how these antibodies are generated humans. We show here appear during infancy correlate with weaning exposure dietary Neu5Gc. However, Neu5Gc alone cannot elicit mice a...
Background Humans are genetically defective in synthesizing the common mammalian sialic acid N-glycolylneuraminic (Neu5Gc), but can metabolically incorporate it from dietary sources (particularly red meat and milk) into glycoproteins glycolipids of human tumors, fetuses some normal tissues. Metabolic incorporation Neu5Gc animal-derived cells medium components also results variable contamination molecules intended for therapies. These Neu5Gc-incorporation phenomena practically significant,...
Two bacterial beta1-4-galactosyltransferases, NmLgtB and Hp1-4GalT, exhibit promiscuous complementary acceptor substrate specificity. They have been used in an efficient one-pot multienzyme system to synthesize LacNAc, lactose, their derivatives including those containing negatively charged 6-O-sulfated GlcNAc C2-substituted or Glc, from monosaccharide inexpensive Glc-1-P.
OPME systems each containing a glycosyltransferase and related sugar activation (SA) enzymes are efficient for synthesizing mammalian carbohydrates derivatives.
Abstract Human carcinomas can metabolically incorporate and present the dietary non-human sialic acid Neu5Gc, which differs from human N-acetylneuraminic (Neu5Ac) by 1 oxygen atom. Tumor-associated Neu5Gc interact with low levels of circulating anti-Neu5Gc antibodies, thereby facilitating tumor progression via chronic inflammation in a human-like Neu5Gc-deficient mouse model. Here we show that antibodies be affinity-purified substantial amounts clinically approved intravenous IgG (IVIG) used...
Both pathogen- and tissue damage-associated molecular patterns induce inflammation through toll-like receptors (TLRs), while sialic acid-binding immunoglobulin superfamily lectin (Siglecs) provide negative regulation. Here we report extensive direct interactions between these pattern recognition receptors. The promiscuous TLR binders were human SIGLEC-5/9 mouse Siglec-3/E/F. Mouse Siglec-G did not show appreciable binding to any TLRs tested. Correspondingly, Siglece deletion enhanced...
An efficient chemoenzymatic synthesis strategy and a HILIC-based purification approach enabled rapid access to an N-glycan isomer library.
Acellular materials of xenogenic origin are used worldwide as xenografts, and phase I trials viable pig pancreatic islets currently being performed. However, limited information is available on transmission porcine endogenous retrovirus (PERV) after xenotransplantation the long-term immune response recipients to xenoantigens. We analyzed blood burn patients who had received living pig-skin dressings for up 8 wk presence PERV well level nature their long term (maximum, 34 y) against Ags....
A promiscuous UDP-sugar pyrophosphorylase (BLUSP) was cloned from Bifidobacterium longum strain ATCC55813 and used efficiently with a Pasteurella multocida inorganic pyrophosphatase (PmPpA) or without monosaccharide 1-kinase for one-pot multienzyme synthesis of UDP-galactose, UDP-glucose, UDP-mannose, their derivatives. Further chemical diversification UDP-mannose derivative resulted in the formation UDP-N-acetylmannosamine.
Salmonella Typhi is an exclusive human pathogen that causes typhoid fever. Typhoid toxin a S. virulence factor can reproduce most of the fever symptoms in experimental animals. Toxicity depends on binding to terminally sialylated glycans surface glycoproteins. Human are unusual because lack CMAH, which other mammals converts N-acetylneuraminic acid (Neu5Ac) N-glycolylneuraminic (Neu5Gc). Here, we report binds and toxic toward cells expressing terminated Neu5Ac (expressed by humans) over...
Influenza viruses cause seasonal flu each year and pandemics or epidemic sporadically, posing a major threat to public health. Recently, new influenza D virus (IDV) was isolated from pigs cattle. Here, we reveal that the IDV utilizes 9-O-acetylated sialic acids as its receptor for entry. Then, determined crystal structures of hemagglutinin-esterase-fusion glycoprotein (HEF) both in free form complex with enzymatic substrate analogs. The HEF shows an extremely similar structural fold...
Bioprosthetic heart valves (BHVs) are commonly used to replace severely diseased but their susceptibility structural valve degeneration (SVD) limits use in young patients. We hypothesized that antibodies against immunogenic glycans present on BHVs, particularly the xenoantigens galactose-α1,3-galactose (αGal) and N-glycolylneuraminic acid (Neu5Gc), could mediate deterioration through calcification. established a large longitudinal prospective international cohort of patients (n = 1668, 34 ±...
In order to understand the biological importance of naturally occurring sialic acid variations on disialyl structures in nature, we developed an efficient two-step multienzyme approach for synthesis a series GD3 ganglioside oligosaccharides and other glycans containing terminal Siaα2−8Sia component with different natural non-natural acids. first step, α2−3- or α2−6-linked monosialylated were obtained using one-pot three-enzyme approach. These compounds then used as acceptors...