A5050152196- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Cancer Immunotherapy and Biomarkers
- Nanoplatforms for cancer theranostics
- Enzyme Production and Characterization
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- Proteoglycans and glycosaminoglycans research
- Infant Nutrition and Health
- Galectins and Cancer Biology
- Luminescence and Fluorescent Materials
- CAR-T cell therapy research
- Molecular Sensors and Ion Detection
- Influenza Virus Research Studies
- Cancer Research and Treatments
- Immune Response and Inflammation
- Seaweed-derived Bioactive Compounds
- Porphyrin and Phthalocyanine Chemistry
- Photodynamic Therapy Research Studies
- T-cell and B-cell Immunology
- Bacteriophages and microbial interactions
- Polysaccharides and Plant Cell Walls
- Cancer Genomics and Diagnostics
Ocean University of China
2021-2025
Qingdao National Laboratory for Marine Science and Technology
2019-2025
Laoshan Laboratory
2023-2024
University of California, Davis
2007-2023
Shandong University
2013-2022
Anhui University
2019-2022
Sichuan University
2020-2022
West China Hospital of Sichuan University
2020-2022
Yibin University
2015-2022
Qingdao Center of Resource Chemistry and New Materials
2020
Human heterophile antibodies that agglutinate animal erythrocytes are known to detect the nonhuman sialic acid N-glycolylneuraminic (Neu5Gc). This monosaccharide cannot by itself fill binding site (paratope) of an antibody and can also be modified presented in various linkages, on diverse underlying glycans. Thus, we hypothesized human anti-Neu5Gc response is polyclonal. Here, use a novel set natural chemoenzymatically synthesized glycans show normal humans have abundant spectrum such...
Abstract Human carcinomas can metabolically incorporate and present the dietary non-human sialic acid Neu5Gc, which differs from human N-acetylneuraminic (Neu5Ac) by 1 oxygen atom. Tumor-associated Neu5Gc interact with low levels of circulating anti-Neu5Gc antibodies, thereby facilitating tumor progression via chronic inflammation in a human-like Neu5Gc-deficient mouse model. Here we show that antibodies be affinity-purified substantial amounts clinically approved intravenous IgG (IVIG) used...
Intracellular lipid metabolism occurs in droplets (LDs), which is critical to the survival of cells. Imaging LDs an intuitive way understand their physiology live However, this limited by availability specific probes that can properly visualize vivo. Here, LDs-specific red-emitting probe proposed address need, not merely with ultrahigh signal-to-noise (S/N) ratio and a large Stokes shift (up 214 nm) but also superior resistance photobleaching. The has been successfully applied real-time...
ConspectusThe limited availability of structurally well-defined diverse glycans remains a major obstacle for deciphering biological functions as well biomedical applications carbohydrates. Despite tremendous progress that has been made in past decades, the synthesis complex still represents one most challenging topics synthetic chemistry. Chemical is time-consuming and labor-intensive process requires elaborate planning skilled personnel. In contrast, glycosyltransferase-catalyzed enzymatic...
Sialyltransferases are key enzymes involved in the biosynthesis of biologically and pathologically important sialic acid-containing molecules nature. Binary X-ray crystal structures a multifunctional Pasteurella multocida sialyltransferase (Δ24PmST1) with donor analogue CMP-3F(a)Neu5Ac or CMP-3F(e)Neu5Ac were determined at 2.0 1.9 Å resolutions, respectively. Ternary protein complex CMP an acceptor lactose have been 2.27 This represents first structure GT-B-type glycosyltransferase that is...
N-Acetylhexosamine 1-kinase (NahK) catalyzes the direct addition of a phosphate from adenosine 5'-triphosphate (ATP) to anomeric position N-acetylhexosamine and shows similar activity towards N-acetylglucosamine (GlcNAc) N-acetylgalactosamine (GalNAc). Herein we report cloning, characterization, substrate specificity studies two NahKs Bifidobacterium infantis ATCC15697 longum ATCC55813, respectively. A new capillary electrophoresis assay method has been developed for enzyme assays. Both...
A highly efficient sequential one-pot multienzyme (OPME) approach for the synthesis of lacto-<italic>N</italic>-neotetraose (LNnT) and its derivatives at preparative scale was reported.
Adoptive transfer of immune cells is being actively pursued for cancer treatment. Natural killer (NK) cells, a class cytotoxic generally lack inherent selectivities toward cancer. To bestow tumor-targeting abilities and enhance anticancer efficacy, new strategy established to glycoengineer NK cells. Carbohydrate-based ligands CD22, marker B cell lymphoma, are introduced onto through either metabolic engineering or glyco-polymer insertion. Such exhibited greatly enhanced cytotoxicities CD22+...
A general, convenient, and efficient enzymatic approach for producing fluorinated mechanistic probes sialidases sialyltransferases is described. Other than the reported sialyltransferase inhibitor CMP-3F(axial)Neu5Ac, CMP-3F(equatorial)Sia derivatives (in which Sia Neu5Ac or Neu5Gc) have been synthesized along with CMP-3F(axial)Neu5Gc CMP-3F(axial)KDN as novel inhibitors important sialyltransferases. 3-Fluoro-sialosides enzymatically could be used potential sialic acid-binding proteins.
A novel and highly efficient chemoenzymatic method has been developed for the preparation of structurally defined macrocyclic oligosaccharides varied sizes. This involves chemical or synthesis containing a galactose at nonreducing end propargyl group reducing as sialyltransferase acceptors. Introducing an azido-containing sialic acid to galactosides through sialyltransferase-catalyzed enzymatic reaction followed by copper(I)-catalyzed Huisgen's 1,3-dipolar cycloaddition alkyne azide provides...
A novel chemoenzymatic approach for the synthesis of disialyl tetrasaccharide epitopes found as terminal oligosaccharides GD1α, GT1aα, and GQ1bα is described. It relies on chemical manipulation enzymatically generated trisaccharides conformationally constrained acceptors regioselective enzymatic α2–6-sialylation. This strategy provides a new route easy access to their derivatives.
Abstract O‐Mannose glycans account up to 30 % of total O‐glycans in the brain. Previous synthesis and functional studies have only focused on core M3 O‐mannose α‐dystroglycan, which are a causative factor for various muscular diseases. In this study, highly efficient chemoenzymatic strategy was developed that enabled first collective 63 M1 M2 glycans. This features gram‐scale chemical five judiciously designed structures, diversity‐oriented modification structures with three enzyme modules...
We demonstrate an efficient, scalable, and stereoselective C-glycosylation with thioglycosides possessing a unique photoactive tetrafluoropyridin-4-yl (TFPy) thio radical leaving group, affording editable medicinally biologically essential C-α-glucogallin derivatives. In the presence of silyl enol ether acceptors, desulfurative coupling reaction performs smoothly under mild conditions upon exposure to blue light irradiation. This versatile protocol permits synthesis sugar-drug chimeras by C1...
Aberrant expression of human sialidases has been shown to associate with various pathological conditions. Despite the effort in sialidase inhibitor design, less attention paid designing specific inhibitors against and characterizing substrate specificity different regarding diverse terminal sialic acid forms sialyl linkages. This is mainly due lack sialoside probes efficient screening methods, as well limited access sialidases. A low cellular level NEU2 hampers its functional inhibitory...