A5072463678- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Biochemical and Molecular Research
- RNA regulation and disease
- Amino Acid Enzymes and Metabolism
- Bacteriophages and microbial interactions
- Nitrogen and Sulfur Effects on Brassica
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Metalloenzymes and iron-sulfur proteins
- Genomics and Phylogenetic Studies
- Dark Matter and Cosmic Phenomena
- Astrophysics and Cosmic Phenomena
- Enzyme Production and Characterization
- RNA Interference and Gene Delivery
- Microbial infections and disease research
- Photosynthetic Processes and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- RNA Research and Splicing
- Plant Pathogens and Fungal Diseases
- Cancer Research and Treatments
- Porphyrin Metabolism and Disorders
- Chemical Synthesis and Analysis
- Metabolism and Genetic Disorders
- Plant Virus Research Studies
University of California, Davis
2016-2025
University of Manchester
2025
University of Cumbria
2025
Newcastle University
2001-2024
Newcastle upon Tyne Hospitals NHS Foundation Trust
2024
Freeman Hospital
2001-2019
University College London
2010
United States Department of Agriculture
2008-2010
Agricultural Research Service
2006-2010
National High Magnetic Field Laboratory
2009
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTX-ray Structures of the Myosin Motor Domain Dictyostelium discoideum Complexed with MgADP.cntdot.BeFx and MgADP.cntdot.AlF4-Andrew J. Fisher, Clyde A. Smith, James Thoden, Robert Kazuo Sutoh, Hazel M. Holden, Ivan RaymentCite this: Biochemistry 1995, 34, 28, 8960–8972Publication Date (Print):July 18, 1995Publication History Published online1 May 2002Published inissue 18 July...
Increased levels of the neutrophil chemokine interleukin (IL)-8 in lungs severe trauma patients can predict subsequent development acute respiratory distress syndrome. Because brain-dead organ donors contain high IL-8, we hypothesized that this may predispose to early graft failure recipient after lung transplantation. Twenty-six prospectively satisfying clinical criteria for donation underwent bronchoalveolar lavage and biopsy determine effect infiltration IL-8 expression donor on function...
Glycosyltransferases are important catalysts for enzymatic and chemoenzymatic synthesis of complex carbohydrates glycoconjugates. The glycosylation efficiencies wild-type glycosyltransferases vary considerably when different acceptor substrates used. Using a multifunctional Pasteurella multocida sialyltransferase 1 (PmST1) as an example, we show here that the sugar nucleotide donor hydrolysis activity contributes significantly to low yield poor substrate is With protein crystal...
Bacterial luciferase is a flavin monooxygenase that catalyzes the oxidation of long-chain aldehyde and releases energy in form visible light. A new crystal cloned from Vibrio harveyi has been grown under low-salt concentrations, which diffract x-rays beyond 1.5-Å resolution. The x-ray structure bacterial refined to conventional R-factor 18.2% for all recorded synchrotron data between 30.0 1.50-Å an α-β heterodimer, individual subunits fold into single domain (β/α)8 barrel. high resolution...
We have examined the growth of human diploid fibroblasts (WI-38 and IMR90) as a function initial seeding density oxygen tension. Cells at young mid-passage levels were subcultivated in Dulbecco's modified Eagle's medium with 10% fetal bovine serum 0.005, 0.01, 0.03, 0.1, 0.3, 1, 2 X 10(4) cells/cm2. Flasks equilibrated before after 1 10 gas mixtures containing desired tension (9-591 mm Hg) placed incubators that measure maintain preset The partial pressure (PO2) media all flasks was...
Sialyltransferases are key enzymes involved in the biosynthesis of biologically and pathologically important sialic acid-containing molecules nature. Binary X-ray crystal structures a multifunctional Pasteurella multocida sialyltransferase (Δ24PmST1) with donor analogue CMP-3F(a)Neu5Ac or CMP-3F(e)Neu5Ac were determined at 2.0 1.9 Å resolutions, respectively. Ternary protein complex CMP an acceptor lactose have been 2.27 This represents first structure GT-B-type glycosyltransferase that is...
Abstract Adenosine deaminases acting on RNA (ADARs) are enzymes that convert adenosine to inosine in duplex RNA, a modification exhibits multitude of effects structure and function. Recent studies have identified ADAR1 as potential cancer therapeutic target. ADARs also important the development directed editing therapeutics. A comprehensive understanding molecular mechanism ADAR reaction will advance efforts develop inhibitors new tools for editing. Here we report X-ray crystal fragment...
The innate immune system relies on molecular sensors to detect distinctive patterns, including viral double-stranded RNA (dsRNA), which triggers responses resulting in apoptosis and infiltration. Adenosine Deaminases Acting (ADARs) catalyze the deamination of adenosine (A) inosine (I), serving as a mechanism distinguish self from non-self prevent aberrant activation. Loss-of-function mutations ADAR1 gene are one cause Aicardi Goutières Syndrome (AGS), severe autoimmune disorder children....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThree-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 .ANG. resolutionAndrew J. Fisher, Frank M. Raushel, Thomas O. Baldwin, and Ivan RaymentCite this: Biochemistry 1995, 34, 20, 6581–6586Publication Date (Print):May 23, 1995Publication History Published online1 May 2002Published inissue 23 1995https://pubs.acs.org/doi/10.1021/bi00020a002https://doi.org/10.1021/bi00020a002research-articleACS PublicationsRequest reuse...
ADARs (adenosine deaminases acting on RNA) can be directed to sites in the transcriptome by complementary guide strands allowing for correction of disease-causing mutations at RNA level. However, show bias against editing adenosines with a guanosine 5' nearest neighbor (5'-GA sites), limiting scope this approach. Earlier studies suggested effect arises from clash minor groove involving 2-amino group adjacent an site. Here we that nucleosides capable pairing syn conformation enhance 5'-GA...
Abstract The [4Fe-4S] cluster is an important cofactor of the base excision repair (BER) adenine DNA glycosylase MutY to prevent mutations associated with 8-oxoguanine (OG). Several MutYs lacking have been identified. Phylogenetic analysis shows that clusterless are distributed in two clades suggesting loss independent evolutionary events. Herein, we determined first crystal structure a complexed DNA. On basis dramatic structural divergence from canonical MutYs, refer this as representative...
At the Ballidon experiment, one of longest running glass durability studies, modern and simulant archaeological glasses were buried in mildly alkaline, under-saturated, conditions for 52 years. Glass surfaces analysed to determine extent mechanisms alteration. Alteration layer chemistry was complex included Ca from surrounding limestone sediment P porewater resulting Ca, Pb Fe-phosphate rich phases interspersed with Si Al regions. There evidence ongoing evolution alteration structure due...
Several proteins with significant identity to ubiquitin have been characterized recently. In contrast ubiquitin's main role in targeting for degradation, a described function of one family ubiquitin-related proteins, the Rub family, is serve as stable post-translational modification complex involved G<sub>1</sub>-to-S cell cycle transition. found animals, plants, and fungi consist 76 residues 52–63% ubiquitin. this study three different RUB within plant Arabidopsis are identified; two differ...
Sialyltransferases catalyze reactions that transfer a sialic acid from CMP−sialic to an acceptor (a structure terminated with galactose, N-acetylgalactosamine, or acid). They are key enzymes the synthesis of acid-containing oligosaccharides, polysaccharides, and glycoconjugates play pivotal roles in many critical physiological pathological processes. The structures truncated multifunctional Pasteurella multocida sialyltransferase (Δ24PmST1), absence presence CMP, have been determined by...
view Abstract Citations (70) References (16) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS 10Be abundance and the age of cosmic rays: a balloon measurement. Hagen, F. A. ; Fisher, J. Ormes, A balloon-borne payload was launched from Thompson, Canada, on August 15, 1973. The float depth averaged 4.5 g/sq cm residual atmosphere, experiment live time 31,500 sec. total-energy-versus-range technique used in measurements. Preliminary results this have been given...