A5078724396- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Enzyme Structure and Function
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Advanced Electron Microscopy Techniques and Applications
- Protein Structure and Dynamics
- Transgenic Plants and Applications
- Genomics and Phylogenetic Studies
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Legume Nitrogen Fixing Symbiosis
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Plant and Fungal Interactions Research
- Virology and Viral Diseases
- Bacterial Genetics and Biotechnology
- Genetics, Aging, and Longevity in Model Organisms
- Respiratory viral infections research
- Mosquito-borne diseases and control
- Evolution and Genetic Dynamics
- Viral Infections and Vectors
- Crystallization and Solubility Studies
Scripps Research Institute
2013-2024
Lundbeck (Denmark)
2023
University of Copenhagen
2023
Scripps Institution of Oceanography
2022-2023
Broadcom (United States)
2022
Martini-Klinik
2019
University Medical Center Hamburg-Eppendorf
2019
Johns Hopkins University
1982-2019
Africa Center
2019
Association of Research Libraries
2019
The crystal structure of the double-stranded DNA bacteriophage HK97 mature empty capsid was determined at 3.6 angstrom resolution. 660 diameter icosahedral particle contains 420 subunits with a new fold. final maturation step is an autocatalytic reaction that creates isopeptide bonds between proteins. Each subunit joined to two its neighbors by ligation side-chain lysine 169 asparagine 356. This generates 12 pentameric and 60 hexameric rings covalently loop through each other, creating...
Grow your nanochemical dendrimers! Cowpea mosaic virus (see relief image derived from the X-ray structure) can be isolated its host plant in gram quantities and participates selective chemical reactions at specific sites of icosahedrally-arrayed coat protein. Mutant structures easily engineered, propagated, to provide particles with tailored reactivities. Applications for these areas catalysis materials science conceived.
Bacteriophages, herpesviruses, and other large double-stranded DNA (dsDNA) viruses contain molecular machines that pump into preassembled procapsids, generating internal capsid pressures exceeding, by 10-fold, of bottled champagne. A 17 angstrom resolution asymmetric reconstruction the infectious P22 virion reveals tightly spooled about portal dodecamer forces a conformation is significantly different from observed in isolated portals assembled ectopically expressed protein. We propose tight...
Of the three domains of life (Eukarya, Bacteria, and Archaea), least understood is Archaea its associated viruses. Many are extremophiles, with species that capable growth at some highest temperatures extremes pH all known organisms. Phylogenetic rRNA-encoding DNA analysis places many hyperthermophilic (species an optimum ≳80°C) base universal tree life, suggesting thermophiles were among first forms on earth. Very few viruses have been identified from as compared to Bacteria Eukarya. We...
Pineal N-acetyltransferase (NAT) activity and radioimmunoassayable levels of melatonin were compared in 2-month-old (young), 12-month-old (middle-aged), 29-month-old (old) female rats killed at 1600 h (during the light) 2300 (4 after darkness onset) 0100 (6 onset). During light period, NAT equivalent pineals from each age group. With onset rose sharply again all groups h. At pineal values old lower than other two groups. Melatonin low glands animals light. By 4 content young had increased...
Different mutants of Cowpea Mosaic Virus (CPMV) have been used as scaffolds to bind 2 and 5 nm gold nanoparticles through gold−sulfur bond formation at specific locations on the virus produce patterns interparticle distances. TEM images confirm that bound particles correlate well with models built from known inserted cysteine groups capsid. These results demonstrate it is possible use CPMV nanoscale place fixed
Cowpea mosaic virus was derivatized with poly(ethylene glycol) to give well-controlled loadings of polymer on the outer surface coat protein assembly. The resulting conjugates displayed altered densities and immunogenicities, consistent known chemical biological properties PEG. These studies make CPMV potentially useful as a tailored vehicle for drug delivery.
Nearly 20 percent of the packaged RNA in bean-pod mottle virus (BPMV) binds to capsid interior a symmetric fashion and is clearly visible electron density map. The displaying icosahedral symmetry single-stranded with well-defined polarity stereochemical properties. Interactions protein are dominated by nonbonding forces few specific contacts. tertiary quaternary structures BPMV proteins similar those observed animal picornaviruses, supporting close relation between plant comoviruses...
Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, cryoelectron microscopy and modeled the change appropriately adapting Head-II. Rigid-body rotations (∼40 degrees) cause switching to an entirely different set interactions; in addition, two motifs undergo refolding. These...
ABSTRACT Porcine circovirus 2 (PCV2) is a T=1 nonenveloped icosahedral virus that has had severe impact on the swine industry. Here we report crystal structure of an N-terminally truncated PCV2 virus-like particle at 2.3-Å resolution, and cryo-electron microscopy (cryo-EM) image reconstruction full-length 9.6-Å resolution. This first atomic circovirus. The revealed capsid protein fold canonical viral jelly roll. loops connecting strands roll define limited features surface. Sulfate ions...
Setting records: Native mass spectometry was used to study the assembly of 18 MDa capsid bacteriophage HK97. The previous record for analysis capsids by this method around 10 MDa. results indicate that efficiency desolvation is main priority in improving native MS instrumentation. As a service our authors and readers, journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized online delivery, but not copy-edited or typeset. Technical...
Archaea and their viruses are poorly understood when compared with the Eukarya Bacteria domains of life. We report here crystal structure major capsid protein (MCP) Sulfolobus turreted icosahedral virus, an archaeal virus isolated from acidic hot spring (pH 2–4, 72–92°C) in Yellowstone National Park. The is nearly identical to MCP structures eukaryotic Paramecium bursaria Chlorella bacteriophage PRD1, shows a common fold mammalian adenovirus. Structural analysis architecture, determined by...