A5038816133- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Blood transfusion and management
- Blood donation and transfusion practices
- Blood groups and transfusion
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Birth, Development, and Health
- Diabetes and associated disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Adipose Tissue and Metabolism
- Immunodeficiency and Autoimmune Disorders
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Respiratory Support and Mechanisms
- Clinical Laboratory Practices and Quality Control
- vaccines and immunoinformatics approaches
- Pregnancy and preeclampsia studies
- Glycosylation and Glycoproteins Research
- Ovarian function and disorders
- Animal Virus Infections Studies
- Long-Term Effects of COVID-19
Stanford Health Care
2018-2025
Stanford Blood Center
2016-2025
Stanford University
2016-2025
Alfred Health
2020-2025
Stanford Medicine
2017-2024
Thai Binh University of Medicine and Pharmacy
2024
Viet Duc Hospital
2024
The University of Texas Health Science Center at San Antonio
2021
Cambridge University Press
2021
New York University Press
2021
SARS-CoV-2-specific antibodies, particularly those preventing viral spike receptor binding domain (RBD) interaction with host angiotensin-converting enzyme 2 (ACE2) receptor, can neutralize the virus. It is, however, unknown which features of serological response may affect clinical outcomes COVID-19 patients. We analyzed 983 longitudinal plasma samples from 79 hospitalized patients and 175 SARS-CoV-2-infected outpatients asymptomatic individuals. Within this cohort, 25 died their illness....
Severe acute respiratory syndrome coronavirus 2 infections can cause disease 2019 (COVID-19), which manifests with a range of severities from mild illness to life-threatening pneumonia and multi-organ failure. COVID-19 is characterized by an inflammatory signature, including high levels cytokines, alveolar infiltrates vascular microthrombi. Here we show that patients severe produced unique serologic increased likelihood IgG1 afucosylated Fc glycans. This modification on IgGs enhanced...
The development of a safe and effective SARS-CoV-2 vaccine is public health priority. We designed subunit candidates using self-assembling ferritin nanoparticles displaying one two multimerized spikes: full-length ectodomain (S-Fer) or C-terminal 70 amino-acid deletion (SΔC-Fer). Ferritin an attractive nanoparticle platform for production vaccines, ferritin-based vaccines have been investigated in humans separate clinical trials. confirmed proper folding antigenicity spike on the surface by...
Clinical diagnosis typically incorporates physical examination, patient history, various laboratory tests, and imaging studies but makes limited use of the human immune system's own record antigen exposures encoded by receptors on B cells T cells. We analyzed receptor datasets from 593 individuals to develop MAchine Learning for Immunological Diagnosis, an interpretive framework screen multiple illnesses simultaneously or precisely test one condition. This approach detects specific...
Uteroplacental insufficiency causes intrauterine growth retardation (IUGR), which is associated with adult onset diseases such as hypertension. Previous studies demonstrate that in humans and rats decreases glomeruli number; however, the molecular mechanisms responsible for this reduction are unknown. Apoptosis plays a key role renal organogenesis. We therefore hypothesized utero deprivation uteroplacental glomeruli, increases apoptosis, alters mRNA levels of apoptosis-related proteins...
Uteroplacental insufficiency leads to intrauterine growth retardation (IUGR) and increases the risk of insulin resistance hypertriglyceridemia in both humans rats. Postnatal changes hepatic gene expression characterize postnatal IUGR rat, despite transient nature initial utero insult. Phenomena such as DNA methylation histone acetylation can induce a relatively static reprogramming transcription by altering chromatin infrastructure. We therefore hypothesized that uteroplacental persistently...
Abstract Elderly humans show decreased humoral immunity to pathogens and vaccines, yet the effects of aging on B cells are not fully known. Chronic viral infection by CMV is implicated as a driver clonal T cell proliferations in some humans, but whether or EBV contributes alterations repertoire with age unclear. We have used high-throughput DNA sequencing IGH gene rearrangements study BCR repertoires over two successive years 27 individuals ranging from 20 89 y. Some features remain stable...
We investigated feasibility and accuracy of an interferon-γ release assay (IGRA) for detection T-cell responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Whole blood IGRA accurately distinguished between convalescent uninfected healthy donors with a predominantly CD4+ response. SARS-CoV-2 may serve as useful diagnostic tool in managing the disease 2019 pandemic.
Distinct environmental exposures are associated with the maturation of antibody types in early childhood.
Vaccination and infection promote the formation, tissue distribution, clonal evolution of B cells, which encode humoral immune memory. We evaluated pediatric adult blood deceased organ donor tissues to determine convergent antigen-specific antibody genes similar sequences shared between individuals. cell memory varied for different pathogens. Polysaccharide clones were not exclusive spleen. Adults had higher clone frequencies greater class switching in lymphoid than blood, while abundant...
Abstract Heart disease is common in both humans and chimpanzees, manifesting typically as sudden cardiac arrest or progressive heart failure. Surprisingly, although chimpanzees are our closest evolutionary relatives, the major cause of different two species. Histopathology data affected chimpanzee hearts from primate centers, analysis literature indicate that death (and gorillas orangutans) commonly associated with diffuse interstitial myocardial fibrosis unknown cause. In contrast, most...
Deep sequence analysis of the IgH repertoires common variable immune deficiency patients highlights phenotypic features disorder and potential disease mechanisms.
The two major mammalian sialic acids are N-acetylneuraminic acid and N-glycolylneuraminic (Neu5Gc). only known biosynthetic pathway generating Neu5Gc is the conversion of CMP-N-acetylneuraminic into CMP-Neu5Gc, which catalyzed by CMP-Neu5Ac hydroxylase enzyme. Given irreversible nature this reaction, there must be pathways for elimination or degradation Neu5Gc, would allow animal cells to adjust levels their needs. Although humans incapable synthesizing due an inactivated CMAH gene,...
Significance In modern hospital systems where complicated, severely ill patient populations are the norm, there is currently no reliable way to forecast use of perishable medical resources enable a smart and economic deliver optimal care. We here demonstrate statistical model using data quantitatively forecast, days in advance, need for platelet transfusions. This approach can be leveraged significantly decrease wastage, and, if adopted nationwide, would save approximately 80 million dollars...
Abstract Uteroplacental insufficiency and subsequent intrauterine growth retardation (IUGR) increase the risk of type 2 diabetes in humans rats. Unsuppressed endogenous hepatic glucose production is a common component insulin resistance associated with diabetes. Peroxisome proliferator-activated receptor-γ coactivator-1 (PGC-1) mediates by controlling mRNA levels glucose-6-phosphatase (G-6-Pase), phosphoenolpyruvate carboxykinase (PEPCK), fructose-1,6-bisphosphatase (FBPase). We therefore...
Abstract SARS-CoV-2-specific antibodies, particularly those preventing viral spike receptor binding domain (RBD) interaction with host angiotensin-converting enzyme 2 (ACE2) receptor, could offer protective immunity, and may affect clinical outcomes of COVID-19 patients. We analyzed 625 serial plasma samples from 40 hospitalized patients 170 SARS-CoV-2-infected outpatients asymptomatic individuals. Severely ill developed significantly higher antibody responses than The development antibodies...