A5014284400- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Metalloenzymes and iron-sulfur proteins
- Trace Elements in Health
- Microbial Metabolic Engineering and Bioproduction
- Corrosion Behavior and Inhibition
- Microbial metabolism and enzyme function
- Metal Extraction and Bioleaching
- Respiratory viral infections research
- Viral Infections and Vectors
- Enzyme Structure and Function
- biodegradable polymer synthesis and properties
- Animal Virus Infections Studies
- Biological Research and Disease Studies
- vaccines and immunoinformatics approaches
- Computational Drug Discovery Methods
- Biochemical and biochemical processes
- Neurological diseases and metabolism
- Metal-Catalyzed Oxygenation Mechanisms
- SARS-CoV-2 detection and testing
- Virology and Viral Diseases
- DNA and Nucleic Acid Chemistry
- Long-Term Effects of COVID-19
- Vibrio bacteria research studies
- Cell death mechanisms and regulation
Stanford University
2020-2024
Universidade Nova de Lisboa
2014-2019
Rede de Química e Tecnologia
2014-2017
SARS-CoV-2-specific antibodies, particularly those preventing viral spike receptor binding domain (RBD) interaction with host angiotensin-converting enzyme 2 (ACE2) receptor, can neutralize the virus. It is, however, unknown which features of serological response may affect clinical outcomes COVID-19 patients. We analyzed 983 longitudinal plasma samples from 79 hospitalized patients and 175 SARS-CoV-2-infected outpatients asymptomatic individuals. Within this cohort, 25 died their illness....
Abstract SARS-CoV-2-specific antibodies, particularly those preventing viral spike receptor binding domain (RBD) interaction with host angiotensin-converting enzyme 2 (ACE2) receptor, could offer protective immunity, and may affect clinical outcomes of COVID-19 patients. We analyzed 625 serial plasma samples from 40 hospitalized patients 170 SARS-CoV-2-infected outpatients asymptomatic individuals. Severely ill developed significantly higher antibody responses than The development antibodies...
Bcl-2 protein is involved in cell apoptosis and considered an interesting target for anti-cancer therapy. The present study aims to understand the stability conformational changes of upon interaction with inhibitor venetoclax, explore other drug-target regions. We combined biophysical silico approaches mechanism ligand binding Bcl-2. Thermal shift assay (TSA) urea electrophoresis showed a significant increase venetoclax incubation, which corroborated by molecular docking dynamics...
The xanthine oxidase (XO) family comprises molybdenum-dependent enzymes that usually form homodimers (or dimers of heterodimers/trimers) organized in three domains harbor two [2Fe-2S] clusters, one FAD, and a Mo cofactor. In this work, we crystallized an unusual member the family, periplasmic aldehyde oxidoreductase PaoABC from Escherichia coli. This is first example E. coli protein containing molybdopterin-cytosine-dinucleotide cofactor only heterotrimer XO so far structurally...
The periplasmic aldehyde oxidoreductase PaoABC from Escherichia coli is a molybdenum enzyme involved in detoxification of aldehydes the cell. It an example αβγ heterotrimeric xanthine oxidase family enzymes which does not dimerize via its cofactor binding domain. In order to structurally characterize PaoABC, X-ray crystallography and small angle scattering (SAXS) have been carried out. protein crystallizes presence 20% (w/v) polyethylene glycol 3350 using hanging-drop vapour diffusion...
Molybdenum and tungsten are taken up by bacteria archaea as their soluble oxyanions through high affinity transport systems belonging to the ATP-binding cassette (ABC) transporters. The component A (ModA/TupA) of these transporters is first selection gate from which cell differentiates between MoO42-, WO42- other similar oxyanions. We report biochemical characterization crystal structure apo-TupA Desulfovibrio desulfuricans G20, at 1.4 Å resolution. Small Angle X-ray Scattering data suggests...
The TupABC system is involved in the cellular uptake of tungsten and belongs to ABC (ATP binding cassette)-type transporter systems. TupA component a periplasmic protein that binds tungstate anions, which are then transported through membrane by TupB using ATP hydrolysis as energy source (the reaction catalyzed ModC component). We report heterologous expression, purification, determination affinity constants crystallization Desulfovibrio alaskensis G20 TupA. tupA gene (locus tag Dde_0234)...
Molybdenum and tungsten enzymes require specific chaperones for folding cofactor insertion. PaoD is the chaperone of periplasmic aldehyde oxidoreductase PaoABC. It last gene in paoABCD operon Escherichia coli its presence crucial obtaining mature enzyme. an unstable, 35 kDa, protein. Our biochemical studies showed that it a dimer solution with tendency to form large aggregates, especially after freezing/thawing cycles. In order improve stability, was thawed two ionic liquids [C4mim]Cl...
SUMMARY During virus infection B cells are critical for the production of antibodies and protective immunity. Here we show that human cell compartment in patients with diagnostically confirmed SARS-CoV-2 clinical COVID-19 is rapidly altered early recruitment expressing a limited subset IGHV genes, progressing to highly polyclonal response broader gene usage extensive class switching IgG IgA subclasses somatic hypermutation initial weeks infection. We identify convergence antibody sequences...
Selective base pairing is the foundation of DNA recognition. Here, we elucidate molecular and structural details a FRET-based two-component beacon relying on steady-state fluorescence spectroscopy, small-angle X-ray scattering (SAXS), microscale thermophoresis (MST), differential electrophoretic mobility. This was designed to detect most common fusion sequences causing chronic myeloid leukemia, e14a2 e13a2. The emission spectra indicate that self-assembly different components biosensor...