A5023153184- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- Biosensors and Analytical Detection
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- HIV Research and Treatment
- Parasitic Diseases Research and Treatment
- Viral-associated cancers and disorders
- Advanced biosensing and bioanalysis techniques
- Parvovirus B19 Infection Studies
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- HIV/AIDS drug development and treatment
- CRISPR and Genetic Engineering
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
- vaccines and immunoinformatics approaches
- Parasites and Host Interactions
- Viral Infections and Immunology Research
Stanford University
2016-2025
National Institute of Science Education and Research
2024
Homi Bhabha National Institute
2024
Stanford Health Care
2017-2024
Regional Medical Research Centre
2024
Advanced Materials Technology (United States)
2024
All India Institute of Medical Sciences Bhubaneswar
2024
Sungkyunkwan University
2024
John Wiley & Sons (Germany)
2023
San Francisco Foundation
2022
SARS-CoV-2-specific antibodies, particularly those preventing viral spike receptor binding domain (RBD) interaction with host angiotensin-converting enzyme 2 (ACE2) receptor, can neutralize the virus. It is, however, unknown which features of serological response may affect clinical outcomes COVID-19 patients. We analyzed 983 longitudinal plasma samples from 79 hospitalized patients and 175 SARS-CoV-2-infected outpatients asymptomatic individuals. Within this cohort, 25 died their illness....
Yan Bai, MD; Lingsheng Yao, Tao Wei, Fei Tian, Dong-Yan Jin, PhD; Lijuan Chen, Meiyun Wang, MD, PhD
Zika virus (ZIKV), chikungunya (CHIKV), and dengue (DENV) cocirculate in Nicaragua. In this study, we sought to compare the quantified viremia clinical presentation of patients infected with 1 or more these viruses. Acute-phase serum samples from 346 a suspected arboviral illness were tested using multiplex real-time reverse-transcription polymerase chain reaction for ZIKV, CHIKV, DENV. Viremia was quantitated each detected virus, information request forms submitted sample recorded. A total...
The rapid spread of COVID-19 across the world has revealed major gaps in our ability to respond new virulent pathogens. Rapid, accurate, and easily configurable molecular diagnostic tests are imperative prevent global diseases. CRISPR-based approaches proving be useful as field-deployable solutions. In one basic form this assay, CRISPR-Cas12 enzyme complexes with a synthetic guide RNA (gRNA). This complex becomes activated only when it specifically binds target DNA cleaves it. thereafter...
Clinical manifestations of Zika virus, chikungunya and dengue virus infections can be similar. To improve detection, streamline molecular workflow, decrease test costs, we developed evaluated a multiplex real-time reverse transcription PCR for these viruses.
BackgroundThere is increasing concern that persistent infection of SARS-CoV-2 within immunocompromised hosts could serve as a reservoir for mutation accumulation and subsequent emergence novel strains with the potential to evade immune responses.MethodsWe describe three patients acute lymphoblastic leukemia who were persistently positive by real-time polymerase chain reaction. Viral viability from longitudinally-collected specimens was assessed. Whole-genome sequencing serological studies...
Dengue virus (DENV) infection can result in severe complications. However, the understanding of molecular correlates severity is limited, partly due to difficulties defining peripheral blood mononuclear cells (PBMCs) that contain DENV RNA vivo. Accordingly, there are currently no biomarkers predictive progression dengue (SD). Bulk transcriptomics data difficult interpret because consists multiple cell types may react differently infection. Here, we applied virus-inclusive single-cell RNA-seq...
Background Dengue fever results from infection with one or more of four different serotypes dengue virus (DENV). Despite the widespread nature this infection, available molecular diagnostics have significant limitations. The aim study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for detection, quantitation, and serotyping viruses in single reaction. Methodology/Principal Findings An rRT-PCR assay targeting 5′ untranslated region capsid gene DENV genome designed...
Background.Infection with any of the 4 related dengue virus serotypes (DENV-1-4) is thought to result in lifelong immunity homotypic reinfection (ie, same serotype).Methods.Archived serum samples collected as part an ongoing pediatric cohort study Nicaragua were tested for DENV by real-time reverse transcription polymerase chain reaction.Samples from 2892 children who presented acute febrile illness clinically attributed a non-DENV cause (hereafter, "C cases").Test results added database...
Abstract Background Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in blood, also known as RNAemia, has been reported, but its prognostic implications are poorly understood. This study aimed to determine the frequency SARS-CoV-2 plasma and association with disease 2019 (COVID-19) clinical severity. Methods An analytical cross-sectional was performed a single-center tertiary care institution included consecutive inpatients outpatients confirmed COVID-19. The...
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets detection. We developed a triplex, real-time SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high testing demands.
ABSTRACT Antiviral therapy for cytomegalovirus (CMV) plays an important role in the clinical management of solid organ and hematopoietic stem cell transplant recipients. However, CMV antiviral can be complicated by drug resistance associated with mutations phosphotransferase UL97 DNA polymerase UL54. We have developed amplicon-based high-throughput sequencing strategy detecting plasma specimens using a microfluidics PCR platform multiplexed library preparation benchtop next-generation...
Abstract SARS-CoV-2 has caused a devastating global pandemic. The recent emergence of variants that are less sensitive to neutralization by convalescent sera or vaccine-induced neutralizing antibody responses raised concerns. A second wave infections in India is leading the expansion variants. B.1.617.1 variant rapidly spread throughout and several countries world. In this study, using live virus assay, we describe response serum from infected vaccinated individuals. We found 6.8-fold more...
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants with concerning phenotypic mutations is public health interest. Genomic surveillance an important tool for a pandemic response, but many laboratories do not have the resources to support population-level sequencing. We hypothesized that nucleic acid amplification test (NAAT) genotype in viral spike protein could facilitate high-throughput variant surveillance. designed and analytically validated one-step...
The coronavirus SARS-CoV-2 (SCV2) causes acute respiratory distress, termed COVID-19 disease, with substantial morbidity and mortality. As SCV2 is related to previously-studied coronaviruses that have been shown the capability for brain invasion, it seems likely may be able do so as well. To date, although there many clinical autopsy-based reports describe a broad range of SCV2-associated neurological conditions, unclear what fraction these due direct CNS invasion versus indirect effects...
Several point-of-care (POC) molecular tests have received emergency use authorization (EUA) from the Food and Drug Administration (FDA) for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The test performance characteristics Accula (Mesa Biotech) SARS-CoV-2 POC need to be evaluated inform its optimal use. aim this study was assess test. assessed by comparing results 100 nasopharyngeal swab samples previously characterized Stanford Health Care EUA...
Different SARS-CoV-2 vaccines are approved in various countries, but few direct comparisons of the antibody responses they stimulate have been reported. We collected plasma specimens July 2021 from 196 Mongolian participants fully vaccinated with one four COVID-19 vaccines: Pfizer/BioNTech, AstraZeneca, Sputnik V, and Sinopharm. Functional testing a panel nine viral variant receptor binding domain (RBD) proteins revealed marked differences vaccine responses, low levels RBD-ACE2 blocking...
Genetic analysis methods are foundational to advancing personalized and preventative medicine, accelerating disease diagnostics, monitoring the health of organisms ecosystems. Current nucleic acid technologies such as polymerase chain reaction (PCR), next-generation sequencing (NGS), DNA microarrays rely on fluorescence absorbance, necessitating sample amplification or replication leading increased processing time cost. Here, we introduce a label-free genetic screening platform based high...
Abstract The rapid emergence of divergent SARS-CoV-2 variants has led to an update the COVID-19 booster vaccine a monovalent version containing XBB.1.5 spike. To determine neutralization breadth following immunization, we collected blood samples from 24 individuals pre- and post-XBB.1.5 mRNA vaccination (∼1 month). improved both neutralizing activity against ancestral strain (WA1) circulating Omicron variants, including EG.5.1, HK.3, HV.1, JN.1. Relative pre-boost titers, induced greater...
In immunosuppressed hosts, the development of multidrug resistance complicates treatment cytomegalovirus (CMV) infection. Improved genotypic detection impending drug may follow from recent technical advances. A severely T-cell-depleted patient with chronic lymphocytic leukemia developed CMV pneumonia and high plasma viral loads that were poorly responsive to antiviral therapy. Serial specimens analyzed for mutant populations by conventional high-throughput deep-sequencing methods....
Highlights•A multi-cohort analysis framework revealed a 20-gene set to predict severe dengue•The 20 genes effectively dengue in existing and prospective cohorts•The is generalizable across ages, host genetic factors, virus strains•The has potential implications for the development of prognostic assaySummaryThere need identify biomarkers predictive dengue. Single-cohort transcriptomics not yielded results or parsimonious, gene sets. We analyzed blood samples patients from seven expression...