A5101574808- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Evolution and Genetic Dynamics
- Biosensors and Analytical Detection
- Genetics, Aging, and Longevity in Model Organisms
- Physiological and biochemical adaptations
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Microbial infections and disease research
- Cellular transport and secretion
- DNA and Nucleic Acid Chemistry
- Streptococcal Infections and Treatments
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Protein Tyrosine Phosphatases
- Alkaline Phosphatase Research Studies
- Monoclonal and Polyclonal Antibodies Research
Stanford University
2020-2024
University of Toronto
2010-2022
Yale University
1993
The rapid spread of COVID-19 across the world has revealed major gaps in our ability to respond new virulent pathogens. Rapid, accurate, and easily configurable molecular diagnostic tests are imperative prevent global diseases. CRISPR-based approaches proving be useful as field-deployable solutions. In one basic form this assay, CRISPR-Cas12 enzyme complexes with a synthetic guide RNA (gRNA). This complex becomes activated only when it specifically binds target DNA cleaves it. thereafter...
Abstract Therapeutic mRNAs and vaccines are being developed for a broad range of human diseases, including COVID-19. However, their optimization is hindered by mRNA instability inefficient protein expression. Here, we describe design principles that overcome these barriers. We develop an RNA sequencing-based platform called PERSIST-seq to systematically delineate in-cell stability, ribosome load, as well in-solution stability library diverse mRNAs. find that, surprisingly, greater driver...
Immunocytochemical and biochemical studies were conducted to characterize a brain-specific protein tyrosine phosphatase, designated STEP for striatal enriched phosphatase. immunoreactivity was most intense in select regions of the CNS receiving dopaminergic input, localized cell bodies, dendrites, axonal processes. Western blot analyses rat brain homogenates revealed triplet polypeptides with relative mobilities (M(r)) 46 kDa, 37 33 kDa within striatum. Phase separation by Triton X-114...
Abstract DNA folding thermodynamics are central to many biological processes and biotechnological applications involving base-pairing. Current methods for predicting stability from sequence use nearest-neighbor models that struggle accurately capture the diverse sequence-dependency of elements other than Watson-Crick base pairs, likely due insufficient experimental data. We introduce a massively parallel method, Array Melt, uses fluorescence-based quenching signals measure equilibrium...
ABSTRACT Type II chromosomal toxin-antitoxin (TA) modules consist of a pair genes that encode two components: stable toxin and labile antitoxin interfering with the lethal action through protein complex formation. Bioinformatic analysis Streptococcus mutans UA159 genome identified linked encoding MazEF-like TA. Our results show S. mazEF form bicistronic operon is cotranscribed from σ70-like promoter. Overproduction MazF had toxic effect on which can be neutralized by coexpression its cognate...
Abstract The rapid spread of COVID-19 across the world has revealed major gaps in our ability to respond new virulent pathogens. Rapid, accurate, and easily configurable molecular diagnostic tests are imperative prevent global diseases. CRISPR-based approaches proving be useful as field-deployable solutions. In a basic form this assay, CRISPR-Cas12 enzyme complexes with synthetic guide RNA (gRNA). This complex is activated when it highly specifically binds target DNA, non-specifically...
SUMMARY Therapeutic mRNAs and vaccines are being developed for a broad range of human diseases, including COVID-19. However, their optimization is hindered by mRNA instability inefficient protein expression. Here, we describe design principles that overcome these barriers. We develop new RNA sequencing-based platform called PERSIST-seq to systematically delineate in-cell stability, ribosome load, as well in-solution stability library diverse mRNAs. find that, surprisingly, greater driver...
Understanding the plasticity, robustness and modularity of transcriptome expression to genetic environmental conditions is crucial deciphering how organisms adapt in nature. To test genome architecture influences profiles, we quantified responses for distinct temperature-adapted genotypes nematode Caenorhabditis briggsae when exposed chronic temperature stresses throughout development. We found that 56% 8,795 differentially expressed genes show genotype-specific changes response...
Geographically distinct populations can adapt to the temperature conditions of their local environment, leading temperature-dependent fitness differences between populations. Consistent with adaptation, phylogeographically Caenorhabditis briggsae nematodes show responses temperature. The genetic mechanisms underlying however, remain unresolved. To investigate potential role small noncoding RNAs in genotype-specific temperature, we quantified RNA expression using high-throughput sequencing C....
Abstract Understanding the plasticity, robustness, and modularity of transcriptome expression to genetic environmental conditions is crucial deciphering how organisms adapt in nature. To test genome architecture influences profiles, we quantified responses for distinct temperature-adapted genotypes nematode Caenorhabditis briggsae when exposed chronic temperature stresses throughout development. We found that 56% 8795 differentially-expressed genes show genotype-specific changes response...
The rapid spread of COVID-19 across the world has revealed major gaps in our ability to respond new virulent pathogens. Rapid, accurate, and easily configurable molecular diagnostic tests are imperative prevent global diseases. CRISPR-based approaches proving be useful as field-deployable solutions. In one basic form this assay, CRISPR-Cas12 enzyme complexes with a synthetic guide RNA (gRNA).This complex becomes activated only when it specifically binds target DNA cleaves it. thereafter...
Abstract Geographically distinct populations can adapt to the temperature conditions of their local environment, leading temperature-dependent fitness differences between populations. Consistent with adaptation, phylogeographically Caenorhabditis briggsae nematodes show responses temperature. The genetic mechanisms underlying however, remain unresolved. To investigate potential role small noncoding RNAs in genotype-specific temperature, we quantified RNA expression using high-throughput...