A5047995826- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Food Allergy and Anaphylaxis Research
- Transgenic Plants and Applications
- Monoclonal and Polyclonal Antibodies Research
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- SARS-CoV-2 and COVID-19 Research
- Kruppel-like factors research
- Reproductive Biology and Fertility
- Phagocytosis and Immune Regulation
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Receptor Mechanisms and Signaling
- Reproductive Physiology in Livestock
- Adipose Tissue and Metabolism
- CAR-T cell therapy research
- Allergic Rhinitis and Sensitization
- Reproductive System and Pregnancy
- Immune cells in cancer
- Atherosclerosis and Cardiovascular Diseases
- Electrolyte and hormonal disorders
- Pregnancy and preeclampsia studies
Indiana University School of Medicine
2016-2022
Indiana University – Purdue University Indianapolis
2016-2022
Stanford University
2020
University of Cincinnati Medical Center
2020
Indiana University
2018-2019
University of Indianapolis
2019
Virginia Tech
2014-2016
Severe acute respiratory syndrome coronavirus 2 infections can cause disease 2019 (COVID-19), which manifests with a range of severities from mild illness to life-threatening pneumonia and multi-organ failure. COVID-19 is characterized by an inflammatory signature, including high levels cytokines, alveolar infiltrates vascular microthrombi. Here we show that patients severe produced unique serologic increased likelihood IgG1 afucosylated Fc glycans. This modification on IgGs enhanced...
Aging results in profound immune dysfunction, resulting the decline of vaccine responsiveness previously attributed to irreversible defects system. In addition increased interleukin-6 (IL-6), we found aged mice exhibit systemic IL-10 that requires forkhead box P3-negative (FoxP3-), but not FoxP3+, CD4+T cells. Most IL-10-producing cells manifested a T follicular helper (Tfh) phenotype and required Tfh cytokines IL-6 IL-21 for their accrual, so refer them as Tfh10 was also maintain normal...
Food allergies are a major clinical problem and driven by IgE antibodies (Abs) specific for food antigens (Ags). T follicular regulatory (Tfr) cells specialized subset of FOXP3+ that modulate Ab responses. Here, we analyzed the role Tfr in regulating Ag-specific using peanut-based allergy model mice. Peanut-specific titers anaphylaxis responses were significantly blunted cell–deficient Foxp3-Cre Bcl6fl/fl Loss led to greatly increased nonspecific levels, showing have both helper suppressor...
T follicular regulatory (TFR) cells are found in the germinal center (GC) response and help shape antibody (Ab) response. However, precise role of TFR GC is controversial. Here, we addressed cell function using mice with impaired development (Bcl6-flox/Foxp3-cre, or Bcl6FC mice), augmented (Blimp1-flox/Foxp3-cre, Blimp1FC two different methods immunization. Unexpectedly, B levels positively correlated levels. Using a gene profiling approach, that TFH from TFR-deficient showed strong...
The production of antibody is precisely controlled during the germinal center (GC) reaction. This process dependent on help from follicular T helper (Tfh) cells to B and regulated by regulatory (Tfr) cells. How Tfr develop how their suppressive activity functions are not well understood. Here, we found that Stat3 indispensible for cell differentiation. After immunization with Sheep Red Blood Cells (SRBC), loss caused deletion in Treg does affect size Tfh or GC population, but rather leads...
A Th2 immune response is central to allergic airway inflammation, which afflicts millions worldwide. However, the mechanisms that augment GATA3 expression in an antigen-primed developing cell are not well understood. Here, we describe unexpected role for Blimp-1, a transcriptional repressor constrains autoimmunity, as upstream promoter of critical development lung inhaled but systemically delivered allergens dispensable TFH function and IgE production. Mechanistically, Blimp-1 acts through...
The transcription factors Bcl6 and Blimp1 have opposing roles in the development of follicular helper T (TFH ) cells: promotes inhibits TFH -cell differentiation. Similarly, activates, while represses, expression marker PD-1. However, repress each other's expression, complicating interpretation regulatory network. Here we sought to clarify extent which independently control differentiation by generating mice with T-cell specific deletion both (double conditional KO [dcKO] mice). Our data...
Immunoglobulin E (IgE) responses are a central feature of allergic disease. Using well-established food-allergy model in mice, we show that two sensitizations with cognate B cell antigen (Ag) and adjuvant 7 days apart promotes optimal development IgE+ germinal center (GC) cells high-affinity IgE production. Intervals 3 or 14 between Ag lead to loss GC an undetectable response. The immunosuppressive factors Fgl2 CD39 down-regulated T follicular helper (TFH) under IgE-sensitization conditions....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections can cause Coronavirus Disease 2019 (COVID-19), which manifests with a range of severities from mild illness to life threatening pneumonia and multi-organ failure. COVID-19 is characterized by an inflammatory signature including high levels cytokines, alveolar infiltrates vascular microthrombi. Here we show that severe patients produced unique serologic signature, increased IgG1 afucosylated Fc glycans. This modification...
Signal regulatory protein (SIRPα) is an immune inhibitory receptor expressed by myeloid cells to inhibit cell phagocytosis, migration, and activation. Despite the progress of SIRPα CD47 antagonist antibodies promote anti-cancer immunity, it not yet known whether agonism could restrain excessive autoimmune tissue inflammation. Here, we report that neutrophil- monocyte-associated genes including SIRPA are increased in inflamed biopsies from patients with rheumatoid arthritis inflammatory bowel...
Uterine secretions are crucial for conceptus development in mammals. This is especially important species that undergo extended preimplantation development, like cattle and other ungulates. The present study examined cooperative interactions epidermal growth factor (EGF), fibroblast factor-2 (FGF2) insulin-like factor-1 (IGF1) on the proliferation of bovine trophoblast cell line CT1 embryo development. Proliferation cells increased after supplementation culture medium with 10ngmL-1 EGF, FGF2...
Clinical studies of type 2 (T2) cytokine-related neutralizing antibodies in asthma have identified a substantial subset patients with low levels T2 inflammation who do not benefit from cytokine antibody treatment. Non-T2 mechanisms are poorly understood but represent redefined unmet medical need.We sought to gain better understanding genetic contributions T2-low asthma.We utilized an unbiased genome-wide association study moderate severe stratified by serum biomarker periostin. We also...
Abstract Autoantibodies can result from excessive T follicular helper (Tfh) cell activity, whereas regulatory (Tfr) cells negatively regulate autoantibody production. IL-2 knockout (KO) mice on the BALB/c background have elevated Tfh responses, produce autoantibodies, and develop lethal autoimmunity. We analyzed Tfr in KO C57BL/6 (B6) genetic background. In B6 mice, spontaneous formation of germinal center B was greatly enhanced, along with production anti-DNA autoantibodies. has been...