A5102962569- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Phagocytosis and Immune Regulation
- Viral gastroenteritis research and epidemiology
- Reproductive System and Pregnancy
- RNA Interference and Gene Delivery
- Bone Metabolism and Diseases
- Biotin and Related Studies
- Pituitary Gland Disorders and Treatments
- Facial Trauma and Fracture Management
- Metal complexes synthesis and properties
- Medical Imaging and Pathology Studies
- Biomarkers in Disease Mechanisms
- RNA modifications and cancer
- Galectins and Cancer Biology
- NF-κB Signaling Pathways
- Bone and Joint Diseases
- Advanced biosensing and bioanalysis techniques
First Affiliated Hospital of Chengdu Medical College
2025
GTx (United States)
2022
Shenzhen Third People’s Hospital
2021
Chang Gung University
2012
Chiayi Chang Gung Memorial Hospital
2012
Guangxi University of Chinese Medicine
2011
Huaqiao University
2011
Genentech
2010
Institute of Neuroimmunology of the Slovak Academy of Sciences
2007
Sun Yat-sen University
2003
Colony stimulating factor 1 (CSF1) and interleukin 34 (IL34) signal via the CSF1 receptor to regulate macrophage differentiation. Studies in IL34- or CSF1-deficient mice have revealed that IL34 function is limited central nervous system skin during development. However, roles of at homeostasis context inflammatory diseases cancer wild-type not been clarified vivo. By neutralizing and/or adult mice, we identified they play important differentiation, specifically steady state microglia,...
Transforming growth factor-β (TGFβ) is a key driver of fibrogenesis. Three TGFβ isoforms (TGFβ1, TGFβ2, and TGFβ3) in mammals have distinct functions embryonic development; however, the postnatal pathological roles activation mechanisms TGFβ2 TGFβ3 not been well characterized. Here, we show that latent forms can be activated by integrin-independent lower thresholds compared to TGFβ1. Unlike TGFB1, TGFB2 TGFB3 expression increased human lung liver fibrotic tissues healthy control tissues....
Significance Statement Specific variants of APOL1, G1 and G2, are associated with CKD in the Black population. Overexpression these kills cells, through different proposed mechanisms subcellular compartments. The localization endogenous APOL1 has not been conclusively established because all studies have used antibodies that crossreact APOL2. Generation use APOL1-specific show podocyte localizes mainly inside endoplasmic reticulum, a few molecules on cell surface. These findings potentially...
The epidermis is a barrier that prevents water loss while keeping harmful substances from penetrating the host. impermeable cornified layer of stratum corneum maintained by balancing continuous turnover driven epidermal basal cell proliferation, suprabasal differentiation, and corneal shedding. desquamation process tightly regulated balance activities serine proteases Kallikrein-related peptidases (KLK) family their cognate inhibitor lymphoepithelial Kazal type-related (LEKTI), which encoded...
Because lentiviruses are able to infect nondividing cells, these viruses might be utilized in gene therapy applications where the target cell does not divide. However, it has been suggested that introduction of primate lentivirus sequences, particularly those human immunodeficiency virus, into cells may pose a health risk for patient. To avoid this concern, we have constructed transfer systems based on nonprimate lentivirus, bovine virus. A panel vectors and packaging constructs was...
Abstract Paired Ig-like type 2 receptor (PILR)α inhibitory and its counterpart PILRβ activating are coexpressed on myeloid cells. In this article, we report that PILRα, but not PILRβ, is elevated in human rheumatoid arthritis synovial tissue correlates with inflammatory cell infiltration. Pilrα−/− mice produce more pathogenic cytokines during inflammation prone to enhanced autoimmune arthritis. Correspondingly, engaging PILRα anti-PILRα mAb ameliorates mouse models suppresses the production...
Clinical studies of type 2 (T2) cytokine-related neutralizing antibodies in asthma have identified a substantial subset patients with low levels T2 inflammation who do not benefit from cytokine antibody treatment. Non-T2 mechanisms are poorly understood but represent redefined unmet medical need.We sought to gain better understanding genetic contributions T2-low asthma.We utilized an unbiased genome-wide association study moderate severe stratified by serum biomarker periostin. We also...
Abstract New therapeutics and combination regimens have led to marked clinical improvements for the treatment of a subset colorectal cancer. Immune checkpoint inhibitors shown efficacy in patients with mismatch-repair–deficient or microsatellite instability–high (MSI-H) metastatic cancer (mCRC). However, microsatellite-stable (MSS) low levels instable (MSI-L) not benefited from these immune modulators, survival outcome remains poor majority diagnosed mCRC. In this article, we describe...
Efferocytosis is a phagocytic process by which apoptotic cells are cleared professional and nonprofessional cells. In tumors, efferocytosis of cancer tumor-associated macrophages prevents Ag presentation suppresses the host immune response against tumor. Therefore, reactivating blockade macrophage-mediated an attractive strategy for immunotherapy. Even though several methods have been developed to monitor efferocytosis, automated high-throughput quantitative assay should offer highly...
Preceding antibody constant regions are switch (S) varying in length and repeat density that targets of activation-induced cytidine deaminase. We asked how participating S influence each other to orchestrate rearrangements at the IgH locus by engineering mice which weakest region, Sε, is replaced with prominent recombination hotspot Sμ. These produce copious polyclonal IgE upon challenge, providing a platform study biology therapeutic interventions. The insertion enhances ε germ-line...
The proactive generation of anti-idiotypic antibodies (anti-IDs) against therapeutic with desirable properties is an important step in pre-clinical and clinical assay development supporting their bioanalytical programs. Here, we describe a robust platform to generate anti-IDs using rabbit single B cell sorting-culture cloning technology by immunizing rabbits drug Fab fragment sorting complementarity determining regions (CDRs) specific cells designed framework control as negative gate exclude...
The methods available to efficiently transduce human CD34+ hematopoietic stem cells (HSCs) derived from mobilized peripheral blood, such that they fully retain their engraftment potential and maintain high levels of transgene expression in vivo, have been unsatisfactory. current murine retrovirus-based gene transfer systems require dividing for efficient transduction, therefore the target HSCs must be activated ex vivo by cytokines cycle, which may limit engrafting ability. Lentivirus-based...
Proteolytic processing of procollagens is a central step during collagen fibril formation. Bone morphogenic protein 1 (BMP1) metalloprotease that plays an important role in the cleavage carboxy-terminal (COOH-terminal) propeptides from procollagens. Although removal required to generate mature fibrils, contribution BMP1 this proteolytic process and its action site remain be fully determined. In study, using postnatal lung fibroblasts as model system, we showed genetic ablation Bmp1 primary...
Abstract Background Osteoporosis is a common phenomenon in HIV patients on tenofovir treatment, but its underlying mechanisms remain to be explored. Methods Quantitative real-time PCR was performed analyze the expression of miR-302, miR-101, miR-145 and osteoclast-specific genes serum treated with ZOL. ELISA used evaluate RANKL, SMAD3 PRKACB these patients. Luciferase assay carried out explore inhibitory effects miR-101 PRKACB, RANKL SMAD3, respectively. Western blot examine involved NF‑κB...
Abstract Purpose A new radiological method was used to evaluate the plastic effect of modified transconjunctival orbital fat decompression surgery in patients with inactive thyroid-associated ophthalmopathy. Methods In this study, 10 (14 eyes) moderate severe ophthalmopathy were selected. The underwent surgery. According results a spiral CT scan before and 6 months after surgery, INFINITT system workstation measure eyeball protrusion value. obtained by PHLIPS IntelliSpace Portal elliptical...
Site specific biotinylation of AviTagged recombinant proteins using BirA enzyme is a widely used protein labeling technology. However, due to the incomplete reactions and lack purification method for biotinylated proteins, it challenging purify sample when mixed with non-biotinylated byproduct. Here, we have developed monoclonal antibody that specifically recognizes AviTag but not sequence. After ten-minute incubation resin conjugated antibody, trapped on while fully material freely passes...
Abstract Idiopathic pulmonary fibrosis (IPF) is a progressive fatal interstitial lung disease that affects three million patients worldwide and currently without an effective cure. Zinpentraxin alfa, recombinant human pentraxin-2 (rhPTX-2) protein, has been evaluated as potential drug candidate for the treatment of IPF. Clinical pharmacokinetic analysis zinpentraxin alfa challenging historically due to interference from serum amyloid P component (SAP), endogenous protein. These molecules...