A5041894622- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Neuroinflammation and Neurodegeneration Mechanisms
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Eosinophilic Disorders and Syndromes
- Cancer Immunotherapy and Biomarkers
- Single-cell and spatial transcriptomics
- Adipose Tissue and Metabolism
- Diabetes and associated disorders
- Pediatric Hepatobiliary Diseases and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Invertebrate Immune Response Mechanisms
- Galectins and Cancer Biology
- Liver Diseases and Immunity
- Adipokines, Inflammation, and Metabolic Diseases
- Gallbladder and Bile Duct Disorders
University of Cincinnati
2015-2023
Cincinnati Children's Hospital Medical Center
2012-2023
University of Cincinnati Medical Center
2013-2021
Children’s Institute
2017
Aging results in profound immune dysfunction, resulting the decline of vaccine responsiveness previously attributed to irreversible defects system. In addition increased interleukin-6 (IL-6), we found aged mice exhibit systemic IL-10 that requires forkhead box P3-negative (FoxP3-), but not FoxP3+, CD4+T cells. Most IL-10-producing cells manifested a T follicular helper (Tfh) phenotype and required Tfh cytokines IL-6 IL-21 for their accrual, so refer them as Tfh10 was also maintain normal...
Regulatory T cells (Tregs), a subset of CD4(+) cells, dramatically accumulate with age in humans and mice contribute to age-related immune suppression. Recently, we showed that majority accumulating Tregs aged expressed low levels CD25, their accrual is associated declining IL-2 mice. In this study, further investigated the origin CD25(lo) First, had high expression neuropilin-1 Helios, broad Vβ repertoire. Next, analyzed gene profile Tregs, naive memory We found were more related young than...
In the multidrug resistance protein 2 (Mdr2) ‐/‐ mouse model, low phospholipid bile instigates biliary epithelial injury, sterile inflammation, and fibrosis, thereby recapitulating disease mechanisms implicated in atresia (BA) primary sclerosing cholangitis. We hypothesize that T lymphocytes contribute to injury fibrosis murine cholangitis (SC) they are susceptible suppression by regulatory cells (Tregs). juvenile Mdr2 mice, intrahepatic CD8+ were expanded, contraction of Tregs coincided...
Cytomegalovirus (CMV) causes a persistent, lifelong infection. CMV persists in latent state and undergoes intermittent subclinical viral reactivation that is quelled by ongoing T cell responses. While cells are critical to maintain control of infection, the immunological factors promote persistence remain unclear. Here, we investigated role regulatory (Treg) mouse model infection using Foxp3-diphtheria toxin receptor (Foxp3-DTR) mice. Eight months after MCMV had established latency spleen,...
Abstract Background Understanding gender-associated bias in aging and obesity-driven metabolic derangements has been hindered by the inability to model severe obesity female mice. Methods Here, using chow- or high fat diet (HFD)-feeding regimens at standard (T S ) thermoneutral N housing temperatures, latter mice, we examined impact of gender on obesity-associated immune responsiveness. Analysis included quantification of: (i) weight gain adiposity; (ii) development severity glucose...
Aging profoundly affects immune-system function, promoting susceptibility to pathogens, cancers and chronic inflammation. We previously identified a population of IL-10-producing, T follicular helper-like cells ("
Abstract Our recent data showed that an aberrant IL-10-producing T follicular helper population (Tfh10) accumulates dramatically with age and is associated age-related declines in vaccine responsiveness. Through single cell gene expression chromatin accessibility analysis of IL-10 + − memory CD4+ cells from young aged mice, we identified increased CD153 on Tfh Tfh10 cells. Mechanistically, linked inflammaging (increased IL-6 levels) to elevated through c-Maf. Surprisingly, blockade mice...
Abstract Type 1 regulatory T cells (Tr1) are a unique population of CD4+ Foxp3− that express high levels IL-10, and have been defined based on their expression CD49b LAG-3. Despite the critical roles played by Tr1 in controlling cell responses autoimmunity infection, mechanisms underlying homeostasis remain unclear. Here, we investigated homeostasis, phenotype function with age. We found accumulated dramatically Further, produced more IL-10 per compared to Foxp3+ Treg. While aged expressed...
Our recent data showed that an aberrant IL-10-producing T follicular helper population (Tfh10) accumulates dramatically with age and is associated age-related declines in vaccine responsiveness. Through single cell gene expression chromatin accessibility analysis of IL-10+ IL-10- memory CD4+ cells from young aged mice, we identified increased CD153 on Tfh Tfh10 cells. Mechanistically, linked inflammaging (increased IL-6 levels) to elevated through c-Maf. Surprisingly, blockade mice...
Abstract Human cytomegalovirus causes a persistent, lifelong infection. The virus persists in latent state and undergoes intermittent subclinical viral reactivation. Such reactivation drives the accumulation of “inflationary” T cells that suppress replication. While are critical to maintain control infection, factors prevent from driving complete elimination remain unclear. Here, we investigated role regulatory (Treg) mouse model persistent CMV infection using FoxP3-diphtheria toxin receptor...