A5031812881- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Congenital Diaphragmatic Hernia Studies
- Diet and metabolism studies
- Infant Nutrition and Health
- Growth Hormone and Insulin-like Growth Factors
- Global Health and Surgery
- Gestational Diabetes Research and Management
- Metabolism and Genetic Disorders
- Digestive system and related health
- Genetics and Neurodevelopmental Disorders
- RNA modifications and cancer
- Stress Responses and Cortisol
- Neonatal and fetal brain pathology
- Health, Environment, Cognitive Aging
- Hormonal Regulation and Hypertension
- Folate and B Vitamins Research
- Global Health Workforce Issues
- Anesthesia and Neurotoxicity Research
- Respiratory Support and Mechanisms
- Diversity and Career in Medicine
- Renal and related cancers
Mercy Research
2020-2024
James J. Peters VA Medical Center
2022-2024
Swinburne University of Technology
2024
Children's Mercy Hospital
2021-2023
University of Minnesota
2018-2023
Mental Illness Research, Education and Clinical Centers
2022
Icahn School of Medicine at Mount Sinai
2022
Medical College of Wisconsin
2013-2019
Children's Hospital of Wisconsin
2013-2019
Triangle
2018
Chromatin structure is epigenetically altered via covalent modifications of histones to allow for heritable gene regulation without altering the nucleotide sequence. Multiple lines evidence from rodents have established a role epigenetic remodeling in regulating transcription response an gestational milieu. However, date, it unknown whether variations intrauterine environment primates similarly induce changes key determinants hepatic chromatin structure. We hypothesized that maternal...
Uteroplacental insufficiency causes intrauterine growth retardation (IUGR), which is associated with adult onset diseases such as hypertension. Previous studies demonstrate that in humans and rats decreases glomeruli number; however, the molecular mechanisms responsible for this reduction are unknown. Apoptosis plays a key role renal organogenesis. We therefore hypothesized utero deprivation uteroplacental glomeruli, increases apoptosis, alters mRNA levels of apoptosis-related proteins...
Uteroplacental insufficiency leads to intrauterine growth retardation (IUGR) and increases the risk of insulin resistance hypertriglyceridemia in both humans rats. Postnatal changes hepatic gene expression characterize postnatal IUGR rat, despite transient nature initial utero insult. Phenomena such as DNA methylation histone acetylation can induce a relatively static reprogramming transcription by altering chromatin infrastructure. We therefore hypothesized that uteroplacental persistently...
Intrauterine growth restriction (IUGR) decreases serum insulin factor-1 (IGF-1) levels. IGF-1 is an epigenetically regulated gene that has two promoters, alternative exon 5 splicing, and multiple termination sites. The regulation of expression involves the whole gene, as evidenced by aforementioned paradigm. We hypothesized IUGR in rat would affect hepatic alter epigenetic characteristics along its length. was induced through a bilateral uterine artery ligation pregnant rat,...
In nonhuman primates, we previously demonstrated that a maternal high-fat diet (MHFD) induces fetal nonalcoholic fatty liver disease (NAFLD) and alters the metabolome. These changes are accompanied by altered acetylation of histone H3 (H3K14ac). However, mechanism behind this alteration in remains unknown. As SIRT1 is both lysine deacetylase crucial sensor cellular metabolism, hypothesized may be involved epigenomic alterations. Here show utero exposure to MHFD, but not obesity per se,...
The effect of in utero exposure to a maternal high-fat diet on the peripheral circadian system fetus is unknown. Using mRNA copy number analysis, we report that components machinery are transcribed nonhuman primate fetal liver an intact phase-antiphase fashion and Npas2, paralog Clock transcription factor, serves as rate-limiting transcript by virtue its relative low abundance (10- 1000-fold lower). We show significantly alters expression hepatic Npas2 (up 7.1-fold, P<0.001) compared with...
Maternal food restriction (FR) during pregnancy results in intrauterine growth-restricted (IUGR) offspring that show rapid catch-up growth and develop metabolic syndrome adult obesity. However, continued nutrient nursing delays prevents development of Epigenetic regulation IGF1, which modulates is synthesized secreted by the liver, may play a role these morbidities. Control (AdLib) pregnant rats received ad libitum through gestation lactation, FR dams were exposed to 50% from days 10 21....
Necrotizing enterocolitis (NEC) is a devastating intestinal disease of premature infants. Although end-stage NEC characterized histopathologically as extensive necrosis, apoptosis may account for the initial loss epithelium before full development disease. We have previously shown that epidermal growth factor (EGF) reduces incidence in rat model. EGF has been to protect enterocytes from apoptosis, mechanism EGF-mediated protection against not known. The aim this study was investigate if...
Intrauterine growth retardation (IUGR) increases the risk of neuroendocrine reprogramming. In rat, IUGR leads to persistent changes in cerebral mRNA levels. This suggests lasting alterations transcriptional regulation, which may result from chromatin structure. Candidate nutritional triggers for these include altered zinc and one-carbon metabolite We hypothesized that affects structure neonatal postnatal rat brains. Rats were rendered by bilateral uterine artery ligation; controls (Con)...
ABSTRACT Introduction : Preliminary evidence in adults with spinal muscular atrophy (SMA) and SMA animal models suggests exercise has potential benefits improving or stabilizing muscle strength motor function. Methods We evaluated feasibility, safety, effects on function of a home‐based, supervised progressive resistance training program children types II III. Up to 14 bilateral proximal muscles were exercised 3 times weekly for 12 weeks. Results Nine SMA, aged 10.4 ± 3.8 years, completed...
Uteroplacental insufficiency and subsequent intrauterine growth retardation (IUGR) increase the risk of adult onset insulin resistance dyslipidemia in humans rats. IUGR rats are further characterized by postnatal alterations hepatic PPAR-gamma coactivator (PGC-1) carnitine-palmitoyl-transferase I (CPTI) expression, as well overall hyperacetylation histone H3. However, it is unknown whether H3 site specific or relates to changes gene expression previously described We therefore hypothesized...
Multiple adult morbidities are associated with intrauterine growth retardation (IUGR) including dyslipidemia. We hypothesized that uteroplacental insufficiency and subsequent IUGR in the rat would lead to altered hepatic fatty acid metabolism. To test this hypothesis, we quantified mRNA levels of acetyl-CoA carboxylase (ACC), carnitine palmitoyltransferase (CPTI), β-oxidation-trifunctional protein (HADH), fasting serum triglycerides, malonyl-CoA at different ages control rats. Fetal gene...
Intrauterine growth restriction (IUGR) increases the risk of serious adult morbidities such as hypertension. In an IUGR rat model hypertension, we reported a persistent decrease in kidney 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) mRNA and protein levels from birth through postnatal (P) day 21. This enzyme deficiency can lead to hypertension by limiting renal glucocorticoid deactivation. present study, hypothesized that affects 11beta-HSD2 epigenetic determinants chromatin...