A5101652981- Folate and B Vitamins Research
- Autism Spectrum Disorder Research
- Child Nutrition and Feeding Issues
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Metabolism and Genetic Disorders
- Family and Disability Support Research
- Birth, Development, and Health
- RNA modifications and cancer
- Esophageal and GI Pathology
- Liver Disease Diagnosis and Treatment
- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
- Gestational Diabetes Research and Management
- Acute Lymphoblastic Leukemia research
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Stress Responses and Cortisol
- Effects of Radiation Exposure
- Tryptophan and brain disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Heavy Metal Exposure and Toxicity
- Cardiovascular Syncope and Autonomic Disorders
- Cystic Fibrosis Research Advances
- Spaceflight effects on biology
University of Arkansas for Medical Sciences
2013-2024
University College London
2024
National Hospital for Neurology and Neurosurgery
2024
King's College Hospital
2020-2022
London Bridge Hospital
2022
Johns Hopkins University
2017-2021
Arkansas Children's Hospital
2010-2020
King's College Hospital NHS Foundation Trust
2019
Queen Mary University of London
2013
University of Arkansas at Little Rock
2012
S-Adenosylmethionine andS-adenosylhomocysteine (SAH), as the substrate and product of essential cellular methyltransferase reactions, are important metabolic indicators methylation status. Chronic elevation SAH, secondary to homocysteine-mediated reversal SAH hydrolase reaction, reduces DNA, RNA, proteins, phospholipids. High affinity binding active site methyltransferases results in inhibition enzyme. Using a sensitive new high pressure liquid chromatography method with coulometric...
Abstract Autism is a behaviorally defined neurodevelopmental disorder usually diagnosed in early childhood that characterized by impairment reciprocal communication and speech, repetitive behaviors, social withdrawal. Although both genetic environmental factors are thought to be involved, none have been reproducibly identified. The metabolic phenotype of an individual reflects the influence endogenous exogenous on genotype. As such, it provides window through which interactive impact genes...
Despite increasing evidence of oxidative stress in the pathophysiology autism, most studies have not evaluated biomarkers within specific brain regions, and functional consequences remain relatively understudied. We examined frozen samples from cerebellum temporal cortex (Brodmann area 22 (BA22)) individuals with autism unaffected controls (n=15 n=12 per group, respectively). Biomarkers stress, including reduced glutathione (GSH), oxidized (GSSG) redox/antioxidant capacity (GSH/GSSG), were...
The impact of abnormal feeding behaviors reported for children with autism spectrum disorders (ASDs) on their nutritional status is unknown. We compared nutrient intake from food consumed by and without ASD examined deficiency excess.Prospective 3-day records BMI (2-11 years) participating in the Autism Treatment Network (Arkansas, Cincinnati, Colorado, Pittsburgh, Rochester) were both National Health Nutrition Examination Survey data a matched subset based age, gender, family income,...
Cerebral folate deficiency (CFD) syndrome is a neurodevelopmental disorder typically caused by receptor autoantibodies (FRAs) that interfere with transport across the blood-brain barrier. Autism spectrum disorders (ASDs) and improvements in ASD symptoms leucovorin (folinic acid) treatment have been reported some children CFD. In ASD, prevalence of FRAs response to FRA-positive has not systematically investigated. this study, serum FRA concentrations were measured 93 high (75.3%) was found....
Research into the metabolic phenotype of autism has been relatively unexplored despite fact that abnormalities have implicated in pathophysiology several other neurobehavioral disorders. Plasma biomarkers oxidative stress reported autistic children; however, intracellular redox status not yet evaluated. Lymphoblastoid cells (LCLs) derived from children and unaffected controls were used to assess relative concentrations reduced glutathione (GSH) oxidized disulfide (GSSG) cell extracts...
The relative changes in plasma and intracellular concentrations of S-adenosylmethionine (SAM) S-adenosylhomocysteine (SAH) may be important predictors cellular methylation potential metabolic alterations associated with specific genetic polymorphisms and/or nutritional deficiencies. Because these metabolites are present nanomolar plasma, methods detection generally require time-consuming precolumn processing or metabolite derivatization.We used HPLC coulometric electrochemical for the...
We report studies of a Croatian boy, proven case human S -adenosylhomocysteine (AdoHcy) hydrolase deficiency. Psychomotor development was slow until his fifth month; thereafter, virtually absent treatment started. He had marked hypotonia with elevated serum creatine kinase and transaminases, prolonged prothrombin time low albumin. Electron microscopy muscle showed numerous abnormal myelin figures; liver biopsy mild hepatitis sparse rough endoplasmic reticulum. Brain MRI at 12.7 months...
Uteroplacental insufficiency leads to intrauterine growth retardation (IUGR) and increases the risk of insulin resistance hypertriglyceridemia in both humans rats. Postnatal changes hepatic gene expression characterize postnatal IUGR rat, despite transient nature initial utero insult. Phenomena such as DNA methylation histone acetylation can induce a relatively static reprogramming transcription by altering chromatin infrastructure. We therefore hypothesized that uteroplacental persistently...
Alcoholic liver disease is associated with abnormal hepatic methionine metabolism and folate deficiency. Because integral to the cycle, its deficiency could promote alcoholic by enhancing ethanol-induced perturbations of DNA damage. We grouped 24 juvenile micropigs receive folate-sufficient (FS) or folate-depleted (FD) diets same containing 40% energy as ethanol (FSE FDE) for 14 wk, significance differences among groups was determined ANOVA. Plasma homocysteine levels were increased in all...
We sought to determine whether high-dose folinic acid improves verbal communication in children with non-syndromic autism spectrum disorder (ASD) and language impairment a double-blind placebo control setting. Forty-eight (mean age 7 years 4 months; 82% male) ASD were randomized receive 12 weeks of (2 mg kg−1 per day, maximum 50 day; n=23) or (n=25). Children subtyped by glutathione folate receptor-α autoantibody (FRAA) status. Improvement communication, as measured ability-appropriate...
Glutathione (GSH) plays an important role in anti-oxidant defense and detoxification reactions. It is primarily synthesized the liver by transsulfuration pathway exported to provide precursors for situ GSH synthesis other tissues. Deficits glutathione have been implicated aging a host of diseases including Alzheimer's disease, Parkinson's cardiovascular cancer, Down syndrome autism.We explore properties metabolism experimenting with mathematical model one-carbon metabolism, pathway,...
There is increasing recognition that mitochondrial dysfunction associated with the autism spectrum disorders. However, little attention has been given to etiology of or how abnormalities might interact other physiological disturbances autism, such as oxidative stress. In current study we used respirometry examine reserve capacity, a measure ability respond stress, in lymphoblastoid cell lines (LCLs) derived from children autistic disorder (AD) well age and gender-matched control LCLs. We...