A5053198596- Trace Elements in Health
- Iron Metabolism and Disorders
- Infant Nutrition and Health
- Heavy Metal Exposure and Toxicity
- Child Nutrition and Water Access
- Breastfeeding Practices and Influences
- MicroRNA in disease regulation
- RNA Research and Splicing
- Pregnancy and preeclampsia studies
- Digestive system and related health
- Neonatal Respiratory Health Research
- RNA modifications and cancer
- Birth, Development, and Health
- Child Nutrition and Feeding Issues
- RNA regulation and disease
- Reproductive System and Pregnancy
- Pregnancy and Medication Impact
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Polar Research and Ecology
- Hernia repair and management
- Calcium signaling and nucleotide metabolism
- Probiotics and Fermented Foods
- Electrolyte and hormonal disorders
Children's Hospital of Philadelphia
2023-2025
University of Massachusetts Lowell
2018-2024
Penn State Milton S. Hershey Medical Center
2012-2021
Pennsylvania State University
2009-2018
Hershey (United States)
2015-2017
Pacific States University
2015
Applied Research Laboratory at Penn State
2012
Park University
2012
University of California, Davis
2001-2011
University of California System
2006-2008
Breast milk normally contains adequate zinc to meet infant requirements up six months of age; however, transient neonatal deficiency has been documented in exclusively breastfed infants women with low concentration. This condition is not corrected by maternal supplementation, supporting the speculation that it results from an inherited genetic condition. We identified a family which two breast-fed developed was associated concentration both women. Sequencing genomic DNA detected mis-sense...
Lactoferrin (Lf) is a major iron-binding and multi-functional protein in exocrine fluids such as breast milk mucosal secretions. The functions of Lf appear dependent upon the iron saturation are postulated to be mediated through internalization by receptor (LfR). However, mechanisms which LfR mediates enterocytes unknown. We now demonstrate that previously cloned from small intestine endocytosis human enterocyte model (Caco-2 cells). was detected at plasma membrane cell surface...
Intrauterine growth retardation (IUGR) increases the risk of neuroendocrine reprogramming. In rat, IUGR leads to persistent changes in cerebral mRNA levels. This suggests lasting alterations transcriptional regulation, which may result from chromatin structure. Candidate nutritional triggers for these include altered zinc and one-carbon metabolite We hypothesized that affects structure neonatal postnatal rat brains. Rats were rendered by bilateral uterine artery ligation; controls (Con)...
ZnT2 (zinc transporter-2) expression is restricted to tissues with unique zinc requirements such as mammary and prostate glands. We previously determined that plays a major role in export from glands, women mutation the gene encoding (SLC30A2) had an approximately 75% reduction milk concentration. Two distinct human isoforms (approximately 42 35 kDa) are predicted result alternative splicing of SLC30A2. examined localization function each isoform, cells generated express ZnT2-HA...
Zinc (Zn) hyper-accumulates in breast tumors and malignant cell lines compared to normal mammary epithelium. The mechanisms responsible for Zn accumulation the consequence of dysregulation are poorly understood. Microarrays were performed assess differences expression transporters metallothioneins (MTs) human cancer lines. Real-time PCR immunoblotting employed profile transporter representative luminal (T47D), basal (MDA-MB-231), non-malignant (MCF10A) distribution was assessed by X-ray...
Zinc is critical for a multitude of cellular processes, including gene expression, secretion and enzymatic activities. Cellular zinc controlled by zinc-chelating proteins transporters. The recent identification permeability the lysosomal ion channel TRPML1 (transient receptor potential mucolipin 1), evidence abnormal levels in cells deficient TRPML1, suggested role transport. In present study we provide new such identify additional components responsible it. agreement with previously...
Zn(2+) is an essential micronutrient and important ionic signal whose excess, as well scarcity, detrimental to cells. Free cytoplasmic controlled by a network of transporters chelating proteins. Recently, lysosomes became the focus studies in transport, they were shown play role Zn(2+)-induced toxicity serving sinks that absorb from cytoplasm. Here, we investigated impact lysosomal sink on net cellular distribution its cell death. We found played cytoprotective during exposure extracellular...
Infants are at a high risk of acquiring fatal infections, and their treatment relies on functioning antibiotics. Antibiotic resistance genes (ARGs) present in numbers antibiotic-naive infants' gut microbiomes, infant mortality caused by resistant infections is high. The role antibiotics shaping the resistome has been studied, but there limited knowledge other factors that affect antibiotic burden gut.Our objectives were to determine impact early exposure formula ARG load neonates infants...
Background Lactoferrin has been suggested to have many biologic activities, such as facilitating iron absorption and having antimicrobial antiinflammatory effects. In humans, several of these activities are likely only be facilitated by human lactoferrin because they depend on the binding specific receptors. Rice may a useful vehicle introduce recombinant infant foods it low allergenicity is safer than using microorganisms or transgenic animals. Methods Recombinant was expressed in rice cell...
The mammary epithelial cell transitions from a non-secreting to terminally differentiated, secreting during lactation. Zinc (Zn) is key modulator of phenotypic transition as it regulates over 300 biological functions including transcription, translation, energy transformation, intracellular signaling, and apoptosis. In addition, Zn must be redirected normal cellular into the secretory compartment, many components system are Zn-dependent an extraordinary amount secreted (1-3 mg Zn/day) milk....
Zinc (Zn) transporter 4 (ZnT4) plays a key role in mammary gland Zn metabolism. A mutation ZnT4 ( SLC30A4) that targets the protein for degradation is responsible “lethal milk” lm/lm) mouse phenotype. only detected secreting gland, and lm/lm mice have ∼35% less milk, decreased size, milk secretion. However, precise contribution of unknown. We used cultured epithelial cells (HC11) determined was localized to trans-Golgi network (TGN) cell membrane transported from cytoplasm. ZnT4-mediated...