A5012643494- Trace Elements in Health
- Calcium signaling and nucleotide metabolism
- Genetic and Kidney Cyst Diseases
- Pancreatic function and diabetes
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Interdisciplinary Research and Collaboration
- Pineapple and bromelain studies
- Diet and metabolism studies
- Epigenetics and DNA Methylation
- Childhood Cancer Survivors' Quality of Life
- Neuropeptides and Animal Physiology
- Plant Micronutrient Interactions and Effects
- Health and Medical Research Impacts
- Lipid Membrane Structure and Behavior
- Protist diversity and phylogeny
- Ion Channels and Receptors
- Health Policy Implementation Science
- Receptor Mechanisms and Signaling
- Cancer Research and Treatment
- Reproductive Biology and Fertility
- Renal and related cancers
- Ethics and Legal Issues in Pediatric Healthcare
- Hedgehog Signaling Pathway Studies
- Genetic Syndromes and Imprinting
Division of Program Coordination Planning and Strategic Initiatives
2020
National Institutes of Health
2020
Yale University
2015-2017
University of Pittsburgh
2013-2014
Zinc is critical for a multitude of cellular processes, including gene expression, secretion and enzymatic activities. Cellular zinc controlled by zinc-chelating proteins transporters. The recent identification permeability the lysosomal ion channel TRPML1 (transient receptor potential mucolipin 1), evidence abnormal levels in cells deficient TRPML1, suggested role transport. In present study we provide new such identify additional components responsible it. agreement with previously...
Zn(2+) is an essential micronutrient and important ionic signal whose excess, as well scarcity, detrimental to cells. Free cytoplasmic controlled by a network of transporters chelating proteins. Recently, lysosomes became the focus studies in transport, they were shown play role Zn(2+)-induced toxicity serving sinks that absorb from cytoplasm. Here, we investigated impact lysosomal sink on net cellular distribution its cell death. We found played cytoprotective during exposure extracellular...
Nearly all cells have a primary cilia on their surface, which functions as cellular antennae. Primary assembly begins intracellularly and eventually emerges extracellularly. However, current ciliogenesis assays, detect length number, do not monitor ciliary stages. We developed new assay that detects antibody access to fluorescently tagged transmembrane protein, revealed three states: classified 'inside,' 'outside,' or 'partial' cilia. Strikingly, most in RPE only partially emerged many...
The critical processes driving successful research translation remain understudied. We describe a mixed-method case study protocol for analyzing translational that has led to the development and implementation of innovative health interventions. An overarching goal these studies is systematically chain events between basic, fundamental scientific discoveries adoption evidence-based applications, including description varied, long-term impacts. approach isolates many key factors enable into...