A5059532776- CRISPR and Genetic Engineering
- HIV/AIDS Research and Interventions
- HIV, Drug Use, Sexual Risk
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- Complementary and Alternative Medicine Studies
- HIV-related health complications and treatments
- Acupuncture Treatment Research Studies
- SARS-CoV-2 detection and testing
- RNA and protein synthesis mechanisms
- HIV/AIDS drug development and treatment
- Pain Management and Placebo Effect
- Muscle Physiology and Disorders
- Biosensors and Analytical Detection
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- COVID-19 Clinical Research Studies
- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Immune responses and vaccinations
- Viral Infections and Immunology Research
Bạch Mai Hospital
2025
University of Florida
2020-2024
Hue University
2024
Duy Tan University
2024
Hung Vuong Hospital
2024
Can Tho University
2020-2024
Princeton University
2024
Fred Hutch Cancer Center
2023
Ho Chi Minh City University of Science
2018-2023
Vietnam National University Ho Chi Minh City
2015-2023
<b>Objective</b> To investigate whether placebo effects can experimentally be separated into the response to three components—assessment and observation, a therapeutic ritual (placebo treatment), supportive patient-practitioner relationship—and then progressively combined produce incremental clinical improvement in patients with irritable bowel syndrome. assess relative magnitude of these components. <b>Design</b> A six week single blind arm randomised controlled trial. <b>Setting</b>...
Abstract The CRISPR-Cas12a RNA-guided complexes have tremendous potential for nucleic acid detection but are limited to the picomolar limit without an amplification step. Here, we develop a platform with engineered crRNAs and optimized conditions that enabled us detect various clinically relevant targets higher sensitivity, achieving of in femtomolar range any target pre-amplification By extending 3′- or 5′-ends crRNA different lengths ssDNA, ssRNA, phosphorothioate discover self-catalytic...
Abstract Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA substrates and is utilized in diagnostic tools identify molecules. We demonstrate that multiple orthologs of Cas12a activate trans-cleavage the presence split activators. Specifically, PAM-distal region crRNA recognizes RNA targets provided PAM-proximal seed target. Our method, Split Activator for Highly Accessible Analysis (SAHARA), detects picomolar concentrations without sample amplification,...
Abstract Introduction The risk of acute kidney injury (AKI) with the use albumin-containing fluids compared to starches in surgical intensive care setting remains uncertain. We evaluated adjusted AKI associated colloids following cardiac surgery. Methods performed a retrospective cohort study patients undergoing on-pump surgery tertiary center from 2008 2010. assessed crystalloid and colloid administration until 36 hours after was defined by RIFLE (risk, injury, failure, loss end-stage...
The coronavirus disease (COVID-19) caused by SARS-CoV-2 has swept through the globe at an unprecedented rate. CRISPR-based detection technologies have emerged as a rapid and affordable platform that can shape future of diagnostics.We developed ENHANCEv2 is composed chimeric guide RNA, modified LbCas12a enzyme, dual reporter construct to improve previously reported ENHANCE system. We validated both using 62 nasopharyngeal swabs compared results RT-qPCR. created lyophilized version...
CRISPR-Cas-based diagnostics have the potential to elevate nucleic acid detection. CRISPR-Cas systems can be combined with a pre-amplification step in one-pot reaction simplify workflow and reduce carryover contamination. Here, we report an engineered Cas12b improved thermostability that falls within optimal temperature range (60°C–65°C) of reverse transcription-loop-mediated isothermal amplification (RT-LAMP). Using de novo structural analyses, introduce mutations wild-type BrCas12b tighten...
Highlights•Engineered crRNAs with variable stem-loop designs demonstrate adaptability Cas12a•cCRISPR broadens the gene editing toolbox by screening 207 combinations of Cas12a and crRNAs•cCRISPR detects SARS-CoV-2 in saliva, nasopharyngeal swabs, tracheal aspiratesSummaryThere is a broad diversity among endonucleases that possess nucleic acid detection gene-editing capabilities, but few are studied extensively. Here, we present an exhaustive investigation 23 orthologs, focus on their cis-...
In 2011, a large outbreak of hand, foot and mouth disease (HFMD) in Vietnam resulted 113,121 children seeking medical attention, whom170 died. Understanding the epidemiology fatal HFMD may improve treatment help targeting prevention activities for vulnerable populations. We describe epidemiological clinical characteristics who died from 2011. Clinical data were obtained through reviewing records deaths occurring January December 2011 all hospitals Vietnam. Hospitals reported any among...
We examined the association of alcohol use disorders (AUD) with adherence to and health-related quality life (HRQOL) outcomes antiretroviral treatment (ART) for HIV/AIDS patients.A cross-sectional multi-site survey was conducted in 468 drug users 648 non-drug (age: 35.4 ± 7.0 years; 63.8% male) 3 epicentres Vietnam. AUD, ART adherence, HRQOL were measured using Alcohol Use Disorders Identification Test - Consumption (AUDIT-C), self-reported Visual Analogue Scale (VAS), World Health...
Vietnam is among the countries with highest rate of HIV transmission through injecting drug users. prevalence users 20% and up to 50% in many provinces. An estimated number country by end 2011 were 171,000 which most common heroin (85%). Detoxification at home, community, rehabilitation centers have been main modalities for managing addiction until Methadone Maintenance Treatment (MMT) was piloted 2008. Recent reports demonstrated positive treatment outcomes. Incidence found remarkably low...
Introduction : Adherence to antiretroviral treatment (ART) is vital in achieving virological success. This study assessed the prevalence of optimal ART adherence and its determinants among HIV/AIDS patients Vietnam. Method A cross-sectional survey was conducted with 1,016 at seven hospitals health centers providing services three provinces, including Hanoi, Hai Phong, Ho Chi Minh City. Self-reported medication measured using a 30-day visual analog scale (VAS) 7-day missed-doses questions....
Health-related quality of life (HRQL) is a good indicator to monitor and evaluate healthcare services for adults with HIV/AIDS. This study described HRQL HIV its determinants, compared it the general population. A cross-sectional national multistage sampling households without HIV-positive people was conducted in 2008. Six provinces were purposively selected represent areas country progressions epidemics. Households sampled probability-proportional-to-size, following selection rural urban...
In our previous study, we demonstrated that headaches are highly prevalent among medical students in Vietnam. the present provide estimates of associated symptom burden and impaired participation, utilizing these to assess headache-related healthcare needs within this population. The study followed standardized methodology established by Global Campaign against Headache. Participants included who were randomly selected from two universities Data collection utilized HARDSHIP questionnaire,...
Retrieving events from videos using text queries has become increasingly challenging due to the rapid growth of multimedia content. Existing methods for text-based video event retrieval often focus heavily on object-level descriptions, overlooking crucial role contextual information. This limitation is especially apparent when lack sufficient context, such as missing location details or ambiguous background elements. To address these challenges, we propose a novel system called RAPID...
1. Mechanically skinned skeletal muscle fibres from the twitch region of iliofibularis cane toads were used to investigate relationship between fibre glycogen content and capacity respond transverse tubular (T-) system depolarization. 2. A large proportion total remained in mechanically exposed aqueous solutions. This pool (about 80% glycogen) was very stable when preparation incubated a rigor solution (pH 7.0) but decreased gradually at rate 0.59+/-0.20% min-1 relaxing (200 nM [Ca2+]). The...
Objectives Vietnam has significantly scaled up its national antiretroviral therapy (ART) program since 2005. With the aim of improving Vietnam's ART program, we conducted an outcome evaluation first five years in this concentrated HIV epidemic where majority persons enrolled care and treatment services are people who inject drugs (PWID). The results may have relevance for other programs with significant PWID populations. Design Retrospective cohort analysis patients at 30 clinics randomly...
Methadone maintenance treatment (MMT) is efficacious in reducing drug use that may improve HIV/AIDS care and outcomes. This study evaluated the incremental cost-effectiveness of MMT for HIV-positive users from perspective health service providers. A sample 370 (age: mean ± SD: 29.5 5.9 years; 95.7% male) taking multi-sites was assessed at baseline, three, six nine months. Costs services were analyzed converted to year 2009. Quality-adjusted life years (QALYs) modeled changes health-related...